Detailed information of TA system TA086447

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 22087..22341
• Replicon: plasmid unnamed1
• Accession: NZ_CP023959
• Organism: Escherichia coli strain FDAARGOS_448

Toxin (Protein)

• Gene name: srnB
• Uniprot ID: -
• Locus tag: CRN68_RS00555
• Protein ID: WP_032215358.1
• Coordinates: 22087..22293 (-)
• Length: 69 a.a.

Antitoxin (RNA)

Gene name	srnC
Locus tag	-
Coordinates	22280..22341 (+)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CRN68_RS00510	17446..17861	-	416	Protein_21	IS1 family transposase	-
CRN68_RS00520	18110..18511	-	402	WP_001398199.1	type II toxin-antitoxin system toxin endoribonuclease PemK	-
CRN68_RS28480	18444..18701	-	258	WP_000557619.1	type II toxin-antitoxin system antitoxin PemI	-
CRN68_RS00525	18794..19447	-	654	WP_000616807.1	CPBP family intramembrane metalloprotease	-
CRN68_RS29175	19545..19685	-	141	WP_001333237.1	hypothetical protein	-
CRN68_RS00535	20386..21243	-	858	WP_029702152.1	incFII family plasmid replication initiator RepA	-
CRN68_RS00540	21236..21310	-	75	WP_001365705.1	RepA leader peptide Tap	-
CRN68_RS00550	21546..21803	-	258	WP_000083833.1	replication regulatory protein RepA	-
CRN68_RS00555	22087..22293	-	207	WP_032215358.1	type I toxin-antitoxin system Hok family toxin	Toxin
-	22280..22341	+	62	NuclAT_0	-	Antitoxin
-	22280..22341	+	62	NuclAT_0	-	Antitoxin
-	22280..22341	+	62	NuclAT_0	-	Antitoxin
-	22280..22341	+	62	NuclAT_0	-	Antitoxin
CRN68_RS29375	22480..22746	-	267	WP_087757657.1	hypothetical protein	-
CRN68_RS00570	23121..23690	-	570	WP_029702150.1	DUF2726 domain-containing protein	-
CRN68_RS00575	23842..24468	-	627	WP_029702149.1	hypothetical protein	-
CRN68_RS00580	24880..25089	-	210	WP_039023209.1	hypothetical protein	-
CRN68_RS00585	25220..25780	-	561	WP_001567328.1	fertility inhibition protein FinO	-
CRN68_RS00590	25835..26581	-	747	WP_052273601.1	conjugal transfer pilus acetylase TraX	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	blaNDM-5 / sul1 / qacE / aadA2 / dfrA12 / qepA4 / tet(B) / blaTEM-1B	-	1..128761	128761

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (23-64)

Sequences

Toxin

Protein

• Length: 69 a.a.
• Molecular weight: 7778.27 Da
• Isoelectric Point: 8.8807

>T86447 WP_032215358.1 NZ_CP023959:c22293-22087 [Escherichia coli]
MKYLNTTDCSLFLAERSKFMTKYALIGVLAVCATVLCFSLIFRERLCELNIHRGNTVVQVTLAYEARK

Nucleotide

• Length: 207 bp

>T86447 NZ_CP023959:c22293-22087 [Escherichia coli]
ATGAAGTACCTTAACACTACTGATTGTAGCCTCTTCCTTGCAGAGAGGTCAAAGTTTATGACGAAATATGCCCTTATCGG
GGTGCTCGCCGTGTGCGCCACGGTGTTGTGTTTTTCACTGATATTCAGGGAACGGTTATGTGAACTGAATATTCACAGGG
GAAATACAGTGGTGCAGGTAACTCTGGCCTACGAAGCACGGAAGTAA

Antitoxin

Nucleotide

• Length: 62 bp

>AT86447 NZ_CP023959:22280-22341 [Escherichia coli]
TTAAGGTACTTCATGCAGACCTCACGAGTTAATGGATTAACGAGTGGGGTCTTCGCATTTCT

Structures

Antitoxin