Detailed information of TA system TA086443

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 31137..31401
• Replicon: plasmid unnamed3
• Accession: NZ_CP023957
• Organism: Escherichia coli strain FDAARGOS_448

Toxin (Protein)

• Gene name: pndA
• Uniprot ID: U9Z417
• Locus tag: CRN68_RS00220
• Protein ID: WP_001387489.1
• Coordinates: 31137..31289 (-)
• Length: 51 a.a.

Antitoxin (RNA)

Gene name	pndB
Locus tag	-
Coordinates	31341..31401 (+)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CRN68_RS00185	26229..26762	-	534	WP_001221666.1	lipocalin family protein	-
CRN68_RS00190	26856..28001	-	1146	WP_099004447.1	CMY-2 family class C beta-lactamase	-
CRN68_RS00195	28454..29151	-	698	Protein_33	IS1 family transposase	-
CRN68_RS00205	29376..29585	-	210	WP_000062603.1	HEAT repeat domain-containing protein	-
CRN68_RS29160	29977..30153	+	177	WP_001054897.1	hypothetical protein	-
CRN68_RS00210	30218..30514	-	297	WP_011264046.1	hypothetical protein	-
CRN68_RS00215	30814..31065	+	252	WP_001291964.1	hypothetical protein	-
CRN68_RS00220	31137..31289	-	153	WP_001387489.1	Hok/Gef family protein	Toxin
-	31341..31401	+	61	NuclAT_0	-	Antitoxin
-	31341..31401	+	61	NuclAT_0	-	Antitoxin
-	31341..31401	+	61	NuclAT_0	-	Antitoxin
-	31341..31401	+	61	NuclAT_0	-	Antitoxin
CRN68_RS00225	31604..31951	+	348	Protein_39	protein finQ	-
CRN68_RS00230	32137..33294	+	1158	Protein_40	IS1380-like element ISEc9 family transposase	-
CRN68_RS00240	33350..34047	+	698	Protein_41	IS1 family transposase	-
CRN68_RS00245	34061..34366	-	306	Protein_42	transcription termination factor NusG	-
CRN68_RS00255	34808..35095	-	288	WP_000074855.1	conjugal transfer protein TraA	-
CRN68_RS00260	35013..35276	-	264	WP_032180564.1	ash family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Mobilizable plasmid	blaCMY-42	-	1..48550	48550
	-	inside	IScluster/Tn	blaCMY-42	-	26856..33625	6769

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (7-46)

Sequences

Toxin

Protein

• Length: 51 a.a.
• Molecular weight: 5761.10 Da
• Isoelectric Point: 8.7948

>T86443 WP_001387489.1 NZ_CP023957:c31289-31137 [Escherichia coli]
MPQRTFLMMLIVVCVTILCFVWMVRDSLCGLRLQQGNTVLVATLAYEVKR

Nucleotide

• Length: 153 bp

>T86443 NZ_CP023957:c31289-31137 [Escherichia coli]
ATGCCACAGCGAACGTTTTTAATGATGTTAATCGTCGTCTGTGTGACGATTCTGTGTTTTGTCTGGATGGTGAGGGATTC
GCTTTGCGGACTCCGGCTCCAGCAGGGAAACACAGTGCTTGTGGCAACGTTAGCCTACGAAGTTAAACGTTAA

Antitoxin

Nucleotide

• Length: 61 bp

>AT86443 NZ_CP023957:31341-31401 [Escherichia coli]
GAGGCATTACAGTAGAGCCCTTGGTTGATTGATAAAAAAATCTCCAGGGGCTTTCTGCTGT

Structures

Toxin

• AlphaFold DB: U9Z417

Antitoxin