Detailed information of TA system TA086426

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/COG4683-HTH_37
• Location: 10908..11565
• Replicon: plasmid unnamed1
• Accession: NZ_CP023948
• Organism: Klebsiella pneumoniae strain FDAARGOS_446

Toxin (Protein)

• Gene name: higB
• Uniprot ID: U3PDC3
• Locus tag: CRN69_RS28240
• Protein ID: WP_000270043.1
• Coordinates: 11215..11565 (-)
• Length: 117 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: CRN69_RS28235
• Protein ID: WP_000124640.1
• Coordinates: 10908..11210 (-)
• Length: 101 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CRN69_RS28185	6542..6970	+	429	WP_000591076.1	hypothetical protein	-
CRN69_RS28190	7028..7387	+	360	WP_000422769.1	hypothetical protein	-
CRN69_RS28195	7387..7833	+	447	WP_000919343.1	hypothetical protein	-
CRN69_RS28200	7830..8348	+	519	WP_000210757.1	nitrite reductase	-
CRN69_RS28205	8348..8578	+	231	WP_000972665.1	hypothetical protein	-
CRN69_RS28210	8565..9422	+	858	WP_001167036.1	hypothetical protein	-
CRN69_RS30560	9334..9639	+	306	WP_171820680.1	hypothetical protein	-
CRN69_RS28220	9642..10169	+	528	WP_004201083.1	thermonuclease family protein	-
CRN69_RS28225	10227..10499	+	273	WP_001043046.1	HU family DNA-binding protein	-
CRN69_RS28230	10588..10881	+	294	WP_001239998.1	hypothetical protein	-
CRN69_RS28235	10908..11210	-	303	WP_000124640.1	XRE family transcriptional regulator	Antitoxin
CRN69_RS28240	11215..11565	-	351	WP_000270043.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
CRN69_RS28245	11728..12276	+	549	WP_001061573.1	hypothetical protein	-
CRN69_RS28255	12617..12811	+	195	WP_000343597.1	hypothetical protein	-
CRN69_RS28260	12822..13193	+	372	WP_000516918.1	hypothetical protein	-
CRN69_RS28265	13186..13656	+	471	WP_001281821.1	hypothetical protein	-
CRN69_RS28270	13671..14006	-	336	WP_000683477.1	hypothetical protein	-
CRN69_RS28275	14103..14591	+	489	WP_001273096.1	DUF1643 domain-containing protein	-
CRN69_RS28280	14594..15091	+	498	WP_000062185.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	dfrA14 / aac(6')-Ib / qacE / sul1 / rmtC / blaNDM-1	htpB	1..219350	219350

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-like (12-106)

Antitoxin

• Cro/C1-type helix-turn-helix domain (33-79)

Sequences

Toxin

Protein

• Length: 117 a.a.
• Molecular weight: 13328.21 Da
• Isoelectric Point: 5.2421

>T86426 WP_000270043.1 NZ_CP023948:c11565-11215 [Klebsiella pneumoniae]
MWVIETTDTFDEWFDALDDTDRANVLASMMVLRDRGPMLSRPYADTVNGSSYSNMKELRVQSKGDPIRAFFAFDPKRKGI
LLCAGNKTGDEKRFYEVMIPIADREFAAHLDKLKKE

Nucleotide

• Length: 351 bp

>T86426 NZ_CP023948:c11565-11215 [Klebsiella pneumoniae]
ATGTGGGTCATCGAGACAACTGACACCTTTGATGAGTGGTTCGATGCACTGGATGATACCGATAGAGCCAACGTGCTGGC
TTCGATGATGGTGCTGCGAGATAGAGGTCCCATGCTGTCGAGACCATACGCGGATACTGTTAACGGTTCATCCTACAGCA
ACATGAAAGAGCTGCGGGTCCAAAGCAAAGGAGATCCTATCAGGGCATTCTTTGCGTTCGATCCAAAGCGTAAGGGGATT
CTTCTCTGCGCCGGTAACAAGACCGGGGACGAGAAAAGGTTTTATGAAGTAATGATCCCAATTGCAGACCGCGAGTTTGC
GGCGCACTTGGATAAATTGAAGAAGGAGTGA

Antitoxin

Protein

• Length: 101 a.a.
• Molecular weight: 11006.73 Da
• Isoelectric Point: 7.2036

>AT86426 WP_000124640.1 NZ_CP023948:c11210-10908 [Klebsiella pneumoniae]
MARTLDQMLATEKPEVVAKAQKAATEMLLNIHLAELRDRMNLTQGEIAASLGVRQPTVSEMEKPGRDLKLSSIKRYVEAS
GGKLRLDVELPDGTHYGFAV

Nucleotide

• Length: 303 bp

>AT86426 NZ_CP023948:c11210-10908 [Klebsiella pneumoniae]
ATGGCAAGAACTCTTGACCAAATGCTGGCAACAGAAAAGCCTGAGGTTGTTGCCAAAGCACAGAAAGCGGCCACTGAGAT
GTTACTGAACATCCACTTGGCAGAGCTTCGTGATCGCATGAACCTTACCCAGGGGGAAATTGCTGCATCTCTGGGTGTGA
GACAGCCGACAGTCTCCGAGATGGAAAAACCTGGACGAGATCTCAAGCTGTCGTCCATTAAGCGGTACGTTGAGGCCTCC
GGTGGCAAGCTACGCCTCGATGTTGAACTTCCGGACGGCACCCACTACGGCTTTGCCGTTTAA

Structures

Toxin

• AlphaFold DB: A0A7G8AFB9