Detailed information of TA system TA086397

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 123869..124295
• Replicon: plasmid unnamed2
• Accession: NZ_CP023942
• Organism: Klebsiella pneumoniae strain FDAARGOS_444

Toxin (Protein)

• Gene name: hok
• Uniprot ID: P11895
• Locus tag: CRN70_RS28680
• Protein ID: WP_001312861.1
• Coordinates: 123869..124027 (-)
• Length: 53 a.a.

Antitoxin (RNA)

Gene name	sok
Locus tag	-
Coordinates	124071..124295 (-)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CRN70_RS28635	118924..119151	-	228	WP_001254386.1	conjugal transfer relaxosome protein TraY	-
CRN70_RS28640	119245..119931	-	687	WP_015059009.1	PAS domain-containing protein	-
CRN70_RS28645	120122..120505	-	384	WP_000124981.1	relaxosome protein TraM	-
CRN70_RS28650	120782..121429	+	648	WP_015059008.1	transglycosylase SLT domain-containing protein	-
CRN70_RS28655	121726..122547	-	822	WP_001234445.1	DUF945 domain-containing protein	-
CRN70_RS28660	122658..122954	-	297	WP_001272251.1	hypothetical protein	-
CRN70_RS28670	123180..123373	-	194	Protein_156	hypothetical protein	-
CRN70_RS28680	123869..124027	-	159	WP_001312861.1	type I toxin-antitoxin system Hok family toxin	Toxin
-	124071..124295	-	225	NuclAT_0	-	Antitoxin
-	124071..124295	-	225	NuclAT_0	-	Antitoxin
-	124071..124295	-	225	NuclAT_0	-	Antitoxin
-	124071..124295	-	225	NuclAT_0	-	Antitoxin
CRN70_RS28690	124307..125026	-	720	WP_001276217.1	plasmid SOS inhibition protein A	-
CRN70_RS28695	125023..125457	-	435	WP_000845953.1	conjugation system SOS inhibitor PsiB	-
CRN70_RS28700	125526..127549	-	2024	Protein_160	ParB/RepB/Spo0J family partition protein	-
CRN70_RS28705	127610..127843	-	234	WP_000006003.1	DUF905 family protein	-
CRN70_RS28710	127901..128428	-	528	WP_000290834.1	single-stranded DNA-binding protein	-
CRN70_RS28720	128656..128849	-	194	Protein_163	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	floR / catA2 / blaKPC-2 / rmtB / blaTEM-1B / blaCTX-M-65	-	1..187926	187926

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (8-48)

Sequences

Toxin

Protein

• Length: 53 a.a.
• Molecular weight: 6082.32 Da
• Isoelectric Point: 9.2584

>T86397 WP_001312861.1 NZ_CP023942:c124027-123869 [Klebsiella pneumoniae]
MKLPRSSLVWCVLIVCLTLLIFTYLTRKSLCEIRYRDGHREVAAFMAYESGK

Nucleotide

• Length: 159 bp

>T86397 NZ_CP023942:c124027-123869 [Klebsiella pneumoniae]
ATGAAACTACCACGAAGTTCCCTTGTCTGGTGTGTGTTGATCGTGTGTCTCACACTGTTGATATTCACTTATCTGACACG
AAAATCGCTGTGCGAGATTCGTTACAGAGACGGACACAGGGAGGTGGCGGCTTTCATGGCTTACGAATCCGGTAAGTAG

Antitoxin

Nucleotide

• Length: 225 bp

>AT86397 NZ_CP023942:c124295-124071 [Klebsiella pneumoniae]
TCACACAGATTACCCGTAAACAGCCTGAATGAGCGGGTTATTTTCAGGAAAAGTGAGTGTGGTCAGCGTGCAGGTATATG
GGCTATGATGTGCCCGGCGCTTGAGGCTTTCTGCCTCATGACGTGAAGGTGGTTTGTTACCGTGTTGTGTGGCAGAAAGA
AGATAGCCCCGTAGTAAGTTAATTTTCATTAACCACCACGAGGCATCCCTATGTCTAGTCCACAT

Structures

Toxin

• AlphaFold DB: P11895

Antitoxin