Detailed information of TA system TA086375

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 30058..30327
• Replicon: plasmid unnamed
• Accession: NZ_CP023918
• Organism: Klebsiella pneumoniae strain FDAARGOS_439

Toxin (Protein)

• Gene name: hok
• Uniprot ID: P11895
• Locus tag: CRN23_RS28765
• Protein ID: WP_001312861.1
• Coordinates: 30169..30327 (+)
• Length: 53 a.a.

Antitoxin (RNA)

Gene name	sok
Locus tag	-
Coordinates	30058..30123 (+)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CRN23_RS28725	25347..25540	+	194	Protein_30	hypothetical protein	-
CRN23_RS28735	25768..26295	+	528	WP_000290834.1	single-stranded DNA-binding protein	-
CRN23_RS28740	26353..26586	+	234	WP_000006003.1	DUF905 family protein	-
CRN23_RS28745	26647..28670	+	2024	Protein_33	ParB/RepB/Spo0J family partition protein	-
CRN23_RS28750	28739..29173	+	435	WP_000845953.1	conjugation system SOS inhibitor PsiB	-
CRN23_RS28755	29170..29889	+	720	WP_001276217.1	plasmid SOS inhibition protein A	-
-	29901..30125	+	225	NuclAT_0	-	-
-	29901..30125	+	225	NuclAT_0	-	-
-	29901..30125	+	225	NuclAT_0	-	-
-	29901..30125	+	225	NuclAT_0	-	-
-	30058..30123	+	66	-	-	Antitoxin
CRN23_RS28765	30169..30327	+	159	WP_001312861.1	type I toxin-antitoxin system Hok family toxin	Toxin
CRN23_RS28775	30823..31016	+	194	Protein_37	hypothetical protein	-
CRN23_RS28785	31242..31538	+	297	WP_001272251.1	hypothetical protein	-
CRN23_RS28790	31649..32470	+	822	WP_001234445.1	DUF945 domain-containing protein	-
CRN23_RS28795	32767..33369	-	603	WP_000243713.1	transglycosylase SLT domain-containing protein	-
CRN23_RS28805	33692..34075	+	384	WP_001354030.1	relaxosome protein TraM	-
CRN23_RS28810	34269..34940	+	672	WP_000283561.1	conjugal transfer transcriptional regulator TraJ	-
CRN23_RS28815	35077..35304	+	228	WP_000089263.1	conjugal transfer relaxosome protein TraY	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	blaCTX-M-15 / dfrA17 / aadA5 / qacE / sul1 / mph(A) / erm(B) / qnrS1	-	1..106914	106914

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (8-48)

Sequences

Toxin

Protein

• Length: 53 a.a.
• Molecular weight: 6082.32 Da
• Isoelectric Point: 9.2584

>T86375 WP_001312861.1 NZ_CP023918:30169-30327 [Klebsiella pneumoniae]
MKLPRSSLVWCVLIVCLTLLIFTYLTRKSLCEIRYRDGHREVAAFMAYESGK

Nucleotide

• Length: 159 bp

>T86375 NZ_CP023918:30169-30327 [Klebsiella pneumoniae]
ATGAAACTACCACGAAGTTCCCTTGTCTGGTGTGTGTTGATCGTGTGTCTCACACTGTTGATATTCACTTATCTGACACG
AAAATCGCTGTGCGAGATTCGTTACAGAGACGGACACAGGGAGGTGGCGGCTTTCATGGCTTACGAATCCGGTAAGTAG

Antitoxin

Nucleotide

• Length: 66 bp

>AT86375 NZ_CP023918:30058-30123 [Klebsiella pneumoniae]
AGAAGATAGCCCCGTAGTAAGTTAATTTTCATTAACCACCACGAGGCATCCCTATGTCTAGTCCAC

Structures

Toxin

• AlphaFold DB: P11895

Antitoxin