Detailed information of TA system TA086345

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 97410..97664
• Replicon: plasmid unnamed1
• Accession: NZ_CP023903
• Organism: Escherichia coli strain FDAARGOS_433

Toxin (Protein)

• Gene name: srnB
• Uniprot ID: -
• Locus tag: CRN16_RS26715
• Protein ID: WP_015387399.1
• Coordinates: 97410..97616 (-)
• Length: 69 a.a.

Antitoxin (RNA)

Gene name	srnC
Locus tag	-
Coordinates	97603..97664 (+)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CRN16_RS26680	92657..93568	-	912	WP_000440183.1	carbamate kinase	-
CRN16_RS26685	93579..94799	-	1221	WP_000410951.1	arginine deiminase	-
CRN16_RS26690	95692..96174	+	483	Protein_106	VENN motif pre-toxin domain-containing protein	-
CRN16_RS26695	96120..96566	-	447	Protein_107	incFII family plasmid replication initiator RepA	-
CRN16_RS26700	96559..96633	-	75	WP_032336874.1	RepA leader peptide Tap	-
CRN16_RS26710	96869..97126	-	258	WP_000083833.1	replication regulatory protein RepA	-
CRN16_RS26715	97410..97616	-	207	WP_015387399.1	type I toxin-antitoxin system Hok family toxin	Toxin
-	97603..97664	+	62	NuclAT_0	-	Antitoxin
-	97603..97664	+	62	NuclAT_0	-	Antitoxin
-	97603..97664	+	62	NuclAT_0	-	Antitoxin
-	97603..97664	+	62	NuclAT_0	-	Antitoxin
CRN16_RS26725	97803..97985	-	183	WP_000968309.1	hypothetical protein	-
CRN16_RS26735	98086..98702	+	617	Protein_112	IS1 family transposase	-
CRN16_RS26740	98740..100311	-	1572	WP_023149734.1	IS66 family transposase	-
CRN16_RS26745	100331..100678	-	348	WP_000624622.1	IS66 family insertion sequence element accessory protein TnpB	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	sitABCD / mph(A) / blaCTX-M-15 / blaOXA-1 / aac(6')-Ib-cr / tet(A)	iutA / iucD / iucC / iucB / iucA	1..101274	101274

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (23-64)

Sequences

Toxin

Protein

• Length: 69 a.a.
• Molecular weight: 7720.23 Da
• Isoelectric Point: 9.3127

>T86345 WP_015387399.1 NZ_CP023903:c97616-97410 [Escherichia coli]
MKYLNTTDCSLFLAGRSKFMTKYALIGLLAVCATVLCFSLIFRERLCELNIHRGNTVVQVTLAYEARK

Nucleotide

• Length: 207 bp

>T86345 NZ_CP023903:c97616-97410 [Escherichia coli]
ATGAAGTACCTTAACACTACTGATTGTAGCCTCTTCCTTGCAGGGAGGTCAAAGTTTATGACGAAATATGCCCTTATCGG
GTTGCTCGCCGTGTGCGCCACGGTGTTGTGTTTTTCACTGATATTCAGGGAACGGTTATGTGAACTGAATATTCACAGGG
GAAATACAGTGGTGCAGGTAACTCTGGCCTACGAAGCACGGAAGTAA

Antitoxin

Nucleotide

• Length: 62 bp

>AT86345 NZ_CP023903:97603-97664 [Escherichia coli]
TTAAGGTACTTCATGCAGACCTCACGAGTTAATGGATTAACGAGTGGGGTCTTCGCATTTCT

Structures

Antitoxin