Detailed information of TA system TA086339

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 4883..5147
• Replicon: plasmid unnamed6
• Accession: NZ_CP023900
• Organism: Escherichia coli strain FDAARGOS_433

Toxin (Protein)

• Gene name: pndA
• Uniprot ID: U9Z417
• Locus tag: CRN16_RS25845
• Protein ID: WP_001387489.1
• Coordinates: 4995..5147 (+)
• Length: 51 a.a.

Antitoxin (RNA)

Gene name	pndB
Locus tag	-
Coordinates	4883..4943 (-)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CRN16_RS25805	1008..1271	+	264	WP_032180564.1	ash family protein	-
CRN16_RS25810	1189..1476	+	288	WP_000074855.1	conjugal transfer protein TraA	-
CRN16_RS25820	1918..2223	+	306	Protein_3	transcription termination factor NusG	-
CRN16_RS25825	2237..2934	-	698	Protein_4	IS1 family transposase	-
CRN16_RS25835	2990..4147	-	1158	Protein_5	IS1380-like element ISEc9 family transposase	-
CRN16_RS25840	4333..4680	-	348	Protein_6	protein finQ	-
-	4883..4943	-	61	NuclAT_0	-	Antitoxin
-	4883..4943	-	61	NuclAT_0	-	Antitoxin
-	4883..4943	-	61	NuclAT_0	-	Antitoxin
-	4883..4943	-	61	NuclAT_0	-	Antitoxin
CRN16_RS25845	4995..5147	+	153	WP_001387489.1	Hok/Gef family protein	Toxin
CRN16_RS25850	5219..5470	-	252	WP_001291964.1	hypothetical protein	-
CRN16_RS25855	5770..6066	+	297	WP_011264046.1	hypothetical protein	-
CRN16_RS27805	6131..6307	-	177	WP_001054897.1	hypothetical protein	-
CRN16_RS25860	6699..6908	+	210	WP_000062603.1	HEAT repeat domain-containing protein	-
CRN16_RS25870	7133..7830	+	698	Protein_12	IS1 family transposase	-
CRN16_RS25875	8283..9428	+	1146	WP_039023136.1	class C extended-spectrum beta-lactamase CMY-42	-
CRN16_RS25880	9522..10055	+	534	WP_001221666.1	lipocalin family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Mobilizable plasmid	blaCMY-42	-	1..34323	34323
	-	inside	IScluster/Tn	blaCMY-42	-	2659..14421	11762

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (7-46)

Sequences

Toxin

Protein

• Length: 51 a.a.
• Molecular weight: 5761.10 Da
• Isoelectric Point: 8.7948

>T86339 WP_001387489.1 NZ_CP023900:4995-5147 [Escherichia coli]
MPQRTFLMMLIVVCVTILCFVWMVRDSLCGLRLQQGNTVLVATLAYEVKR

Nucleotide

• Length: 153 bp

>T86339 NZ_CP023900:4995-5147 [Escherichia coli]
ATGCCACAGCGAACGTTTTTAATGATGTTAATCGTCGTCTGTGTGACGATTCTGTGTTTTGTCTGGATGGTGAGGGATTC
GCTTTGCGGACTCCGGCTCCAGCAGGGAAACACAGTGCTTGTGGCAACGTTAGCCTACGAAGTTAAACGTTAA

Antitoxin

Nucleotide

• Length: 61 bp

>AT86339 NZ_CP023900:c4943-4883 [Escherichia coli]
GAGGCATTACAGTAGAGCCCTTGGTTGATTGATAAAAAAATCTCCAGGGGCTTTCTGCTGT

Structures

Toxin

• AlphaFold DB: U9Z417

Antitoxin