Detailed information of TA system TA086315

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 80993..81262
• Replicon: plasmid unnamed2
• Accession: NZ_CP023895
• Organism: Escherichia coli strain FDAARGOS_433

Toxin (Protein)

• Gene name: hok
• Uniprot ID: P11895
• Locus tag: CRN16_RS00525
• Protein ID: WP_001312861.1
• Coordinates: 81104..81262 (+)
• Length: 53 a.a.

Antitoxin (RNA)

Gene name	sok
Locus tag	-
Coordinates	80993..81058 (+)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CRN16_RS00495	76703..77230	+	528	WP_000290834.1	single-stranded DNA-binding protein	-
CRN16_RS00500	77288..77521	+	234	WP_000006003.1	DUF905 family protein	-
CRN16_RS00505	77582..79605	+	2024	Protein_91	ParB/RepB/Spo0J family partition protein	-
CRN16_RS00510	79674..80108	+	435	WP_000845953.1	conjugation system SOS inhibitor PsiB	-
CRN16_RS00515	80105..80824	+	720	WP_001276217.1	plasmid SOS inhibition protein A	-
-	80836..81060	+	225	NuclAT_0	-	-
-	80836..81060	+	225	NuclAT_0	-	-
-	80836..81060	+	225	NuclAT_0	-	-
-	80836..81060	+	225	NuclAT_0	-	-
CRN16_RS27600	80845..81024	-	180	WP_001309233.1	hypothetical protein	-
-	80993..81058	+	66	-	-	Antitoxin
CRN16_RS00525	81104..81262	+	159	WP_001312861.1	type I toxin-antitoxin system Hok family toxin	Toxin
CRN16_RS27845	81500..81877	-	378	Protein_96	hypothetical protein	-
CRN16_RS00545	82177..82473	+	297	WP_001272251.1	hypothetical protein	-
CRN16_RS00550	82584..83405	+	822	WP_001234445.1	DUF945 domain-containing protein	-
CRN16_RS00555	83702..84304	-	603	WP_000243713.1	transglycosylase SLT domain-containing protein	-
CRN16_RS00565	84627..85010	+	384	WP_001354030.1	relaxosome protein TraM	-
CRN16_RS00570	85204..85875	+	672	WP_000283561.1	conjugal transfer transcriptional regulator TraJ	-
CRN16_RS00575	86012..86239	+	228	WP_000089263.1	conjugal transfer relaxosome protein TraY	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	mph(A) / blaTEM-1B / rmtB / blaNDM-5 / sul1 / qacE / aadA2 / dfrA12	-	1..91648	91648

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (8-48)

Sequences

Toxin

Protein

• Length: 53 a.a.
• Molecular weight: 6082.32 Da
• Isoelectric Point: 9.2584

>T86315 WP_001312861.1 NZ_CP023895:81104-81262 [Escherichia coli]
MKLPRSSLVWCVLIVCLTLLIFTYLTRKSLCEIRYRDGHREVAAFMAYESGK

Nucleotide

• Length: 159 bp

>T86315 NZ_CP023895:81104-81262 [Escherichia coli]
ATGAAACTACCACGAAGTTCCCTTGTCTGGTGTGTGTTGATCGTGTGTCTCACACTGTTGATATTCACTTATCTGACACG
AAAATCGCTGTGCGAGATTCGTTACAGAGACGGACACAGGGAGGTGGCGGCTTTCATGGCTTACGAATCCGGTAAGTAG

Antitoxin

Nucleotide

• Length: 66 bp

>AT86315 NZ_CP023895:80993-81058 [Escherichia coli]
AGAAGATAGCCCCGTAGTAAGTTAATTTTCATTAACCACCACGAGGCATCCCTATGTCTAGTCCAC

Structures

Toxin

• AlphaFold DB: P11895

Antitoxin