Detailed information of TA system TA086305

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 70265..70504
• Replicon: plasmid p2_0.1
• Accession: NZ_CP023854
• Organism: Escherichia coli strain 2/0

Toxin (Protein)

• Gene name: srnB
• Uniprot ID: A0A762TWR7
• Locus tag: CRT29_RS26260
• Protein ID: WP_023144756.1
• Coordinates: 70370..70504 (+)
• Length: 45 a.a.

Antitoxin (RNA)

Gene name	sok
Locus tag	-
Coordinates	70265..70325 (-)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CRT29_RS26235	67795..68541	+	747	WP_000205718.1	conjugal transfer pilus acetylase TraX	-
CRT29_RS26240	68596..69156	+	561	WP_000139341.1	fertility inhibition protein FinO	-
CRT29_RS26245	69287..69499	+	213	WP_013023861.1	hypothetical protein	-
CRT29_RS26860	70012..70298	+	287	Protein_84	DUF2726 domain-containing protein	-
-	70265..70325	-	61	NuclAT_2	-	Antitoxin
-	70265..70325	-	61	NuclAT_2	-	Antitoxin
-	70265..70325	-	61	NuclAT_2	-	Antitoxin
-	70265..70325	-	61	NuclAT_2	-	Antitoxin
CRT29_RS26260	70370..70504	+	135	WP_023144756.1	type I toxin-antitoxin system Hok family toxin	Toxin
CRT29_RS26265	70801..71055	+	255	WP_000083850.1	replication regulatory protein RepA	-
CRT29_RS26270	71215..71961	-	747	WP_001016257.1	IS21-like element ISEc12 family helper ATPase IstB	-
CRT29_RS26275	71976..73517	-	1542	WP_002431311.1	IS21-like element ISEc12 family transposase	-
CRT29_RS26980	73748..73882	+	135	Protein_89	protein CopA/IncA	-
CRT29_RS26285	73879..73953	+	75	WP_014966221.1	RepA leader peptide Tap	-
CRT29_RS26290	73946..74803	+	858	WP_000130646.1	incFII family plasmid replication initiator RepA	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	aadA5 / qacE / sul1 / mph(A) / blaTEM-1B / tet(B)	-	1..135053	135053

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (4-44)

Sequences

Toxin

Protein

• Length: 45 a.a.
• Molecular weight: 4896.90 Da
• Isoelectric Point: 7.7209

>T86305 WP_023144756.1 NZ_CP023854:70370-70504 [Escherichia coli]
MTKYTLIGLLAVCATVLCFSLIFREQLCELNIHRGNTVVQVTLA

Nucleotide

• Length: 135 bp

>T86305 NZ_CP023854:70370-70504 [Escherichia coli]
ATGACGAAATATACCCTTATCGGGTTGCTTGCCGTGTGCGCCACGGTGTTGTGTTTTTCACTGATATTCAGGGAACAGTT
ATGTGAACTGAATATTCACAGGGGAAATACAGTGGTGCAGGTAACTCTGGCCTGA

Antitoxin

Nucleotide

• Length: 61 bp

>AT86305 NZ_CP023854:c70325-70265 [Escherichia coli]
TAAAGTGCATCATGCAGACCTCACGAGTTAATGGATTAACGAGTGGGGTCTTCGCATTTCT

Structures

Toxin

• AlphaFold DB: A0A762TWR7

Antitoxin