Detailed information of TA system TA086301

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 24276..24516
• Replicon: plasmid p2_0.1
• Accession: NZ_CP023854
• Organism: Escherichia coli strain 2/0

Toxin (Protein)

• Gene name: hok
• Uniprot ID: P11895
• Locus tag: CRT29_RS25925
• Protein ID: WP_001312861.1
• Coordinates: 24358..24516 (+)
• Length: 53 a.a.

Antitoxin (RNA)

Gene name	sok
Locus tag	-
Coordinates	24276..24314 (+)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CRT29_RS25905	19559..21517	+	1959	WP_000117179.1	ParB/RepB/Spo0J family partition protein	-
CRT29_RS25910	21572..22006	+	435	WP_000845940.1	conjugation system SOS inhibitor PsiB	-
CRT29_RS25915	22003..22722	+	720	WP_001276232.1	plasmid SOS inhibition protein A	-
-	22734..22836	+	103	NuclAT_0	-	-
-	22734..22836	+	103	NuclAT_0	-	-
-	22734..22836	+	103	NuclAT_0	-	-
-	22734..22836	+	103	NuclAT_0	-	-
CRT29_RS25920	22882..24251	+	1370	WP_085947770.1	IS3-like element IS150 family transposase	-
-	24276..24314	+	39	NuclAT_1	-	Antitoxin
-	24276..24314	+	39	NuclAT_1	-	Antitoxin
-	24276..24314	+	39	NuclAT_1	-	Antitoxin
-	24276..24314	+	39	NuclAT_1	-	Antitoxin
CRT29_RS25925	24358..24516	+	159	WP_001312861.1	type I toxin-antitoxin system Hok family toxin	Toxin
CRT29_RS26970	24959..25294	+	336	WP_013023876.1	hypothetical protein	-
CRT29_RS26975	25207..25413	+	207	WP_000275859.1	hypothetical protein	-
CRT29_RS25945	25438..25725	+	288	WP_000107535.1	hypothetical protein	-
CRT29_RS25950	25844..26665	+	822	WP_001234469.1	DUF945 domain-containing protein	-
CRT29_RS25955	26962..27564	-	603	WP_000243712.1	transglycosylase SLT domain-containing protein	-
CRT29_RS25960	27895..28278	+	384	WP_001151566.1	relaxosome protein TraM	-
CRT29_RS25965	28412..29089	+	678	WP_001348626.1	PAS domain-containing protein	-
CRT29_RS25970	29177..29404	+	228	WP_001254388.1	conjugal transfer relaxosome protein TraY	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	aadA5 / qacE / sul1 / mph(A) / blaTEM-1B / tet(B)	-	1..135053	135053

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (8-48)

Sequences

Toxin

Protein

• Length: 53 a.a.
• Molecular weight: 6082.32 Da
• Isoelectric Point: 9.2584

>T86301 WP_001312861.1 NZ_CP023854:24358-24516 [Escherichia coli]
MKLPRSSLVWCVLIVCLTLLIFTYLTRKSLCEIRYRDGHREVAAFMAYESGK

Nucleotide

• Length: 159 bp

>T86301 NZ_CP023854:24358-24516 [Escherichia coli]
ATGAAACTACCACGAAGTTCCCTTGTCTGGTGTGTGTTGATCGTGTGTCTCACACTGTTGATATTCACTTATCTGACACG
AAAATCGCTGTGCGAGATTCGTTACAGAGACGGACACAGAGAGGTGGCGGCTTTCATGGCTTACGAATCCGGTAAGTAG

Antitoxin

Nucleotide

• Length: 39 bp

>AT86301 NZ_CP023854:24276-24314 [Escherichia coli]
CATTAACCACCACGAGGCATCCCTATGTCTAGTCCACAT

Structures

Toxin

• AlphaFold DB: P11895

Antitoxin