Detailed information of TA system TA086260

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 39209..39635
• Replicon: plasmid p4_0.1
• Accession: NZ_CP023850
• Organism: Escherichia coli strain 4/0

Toxin (Protein)

• Gene name: hok
• Uniprot ID: P11895
• Locus tag: CRT34_RS26470
• Protein ID: WP_001312861.1
• Coordinates: 39209..39367 (-)
• Length: 53 a.a.

Antitoxin (RNA)

Gene name	sok
Locus tag	-
Coordinates	39411..39635 (-)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CRT34_RS26420	34247..34474	-	228	WP_001254388.1	conjugal transfer relaxosome protein TraY	-
CRT34_RS26425	34568..35254	-	687	WP_000332487.1	PAS domain-containing protein	-
CRT34_RS26430	35448..35831	-	384	WP_001151564.1	relaxosome protein TraM	-
CRT34_RS26435	36117..36764	+	648	WP_000614282.1	transglycosylase SLT domain-containing protein	-
CRT34_RS26440	37061..37882	-	822	WP_001234469.1	DUF945 domain-containing protein	-
CRT34_RS26445	38003..38290	-	288	WP_000107537.1	hypothetical protein	-
CRT34_RS26470	39209..39367	-	159	WP_001312861.1	type I toxin-antitoxin system Hok family toxin	Toxin
-	39411..39635	-	225	NuclAT_0	-	Antitoxin
-	39411..39635	-	225	NuclAT_0	-	Antitoxin
-	39411..39635	-	225	NuclAT_0	-	Antitoxin
-	39411..39635	-	225	NuclAT_0	-	Antitoxin
CRT34_RS28045	39447..39635	+	189	WP_001299721.1	hypothetical protein	-
CRT34_RS26475	39647..40366	-	720	WP_001276234.1	plasmid SOS inhibition protein A	-
CRT34_RS26480	40363..40797	-	435	WP_000845901.1	conjugation system SOS inhibitor PsiB	-
CRT34_RS26485	40852..42810	-	1959	WP_001145469.1	ParB/RepB/Spo0J family partition protein	-
CRT34_RS26490	42869..43102	-	234	WP_000006004.1	DUF905 domain-containing protein	-
CRT34_RS26495	43158..43685	-	528	WP_000290793.1	single-stranded DNA-binding protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	aadA5 / qacE / sul1 / mph(A) / aac(3)-IIa / blaCTX-M-15 / blaOXA-1 / aac(6')-Ib-cr / tet(A)	-	1..138672	138672

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (8-48)

Sequences

Toxin

Protein

• Length: 53 a.a.
• Molecular weight: 6082.32 Da
• Isoelectric Point: 9.2584

>T86260 WP_001312861.1 NZ_CP023850:c39367-39209 [Escherichia coli]
MKLPRSSLVWCVLIVCLTLLIFTYLTRKSLCEIRYRDGHREVAAFMAYESGK

Nucleotide

• Length: 159 bp

>T86260 NZ_CP023850:c39367-39209 [Escherichia coli]
ATGAAACTACCACGAAGTTCCCTTGTCTGGTGTGTGTTGATCGTGTGTCTCACACTGTTGATATTCACTTATCTGACACG
AAAATCGCTGTGCGAGATTCGTTACAGAGACGGACACAGGGAGGTGGCGGCTTTCATGGCTTACGAATCCGGTAAGTAG

Antitoxin

Nucleotide

• Length: 225 bp

>AT86260 NZ_CP023850:c39635-39411 [Escherichia coli]
TCACACGGATTTCCCGTGAACGGTCTGAATGAGCGGATTATTTTCAGGGAAAGTGAGTGTGGTCAGCGTGCAGGTATATG
GGCTATGATGTGCCCGGCGCTTGAGGCTTTCTGCCTCATGACGTGAAGGTGGTTTGTTGCCGTGTTGTGTGGCAGAAAGA
AGATAGCCCCGTAGTAAGTTAATTTTCATTAACCACCACGAGGCATCCCTATGTCTAGTCCACAT

Structures

Toxin

• AlphaFold DB: P11895

Antitoxin