Detailed information of TA system TA086253

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HTH(antitoxin)
• Location: 4460962..4461564
• Replicon: chromosome
• Accession: NZ_CP023849
• Organism: Escherichia coli strain 4/0

Toxin (Protein)

• Gene name: higB
• Uniprot ID: S1P416
• Locus tag: CRT34_RS22350
• Protein ID: WP_000897302.1
• Coordinates: 4460962..4461273 (+)
• Length: 104 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: CRT34_RS22355
• Protein ID: WP_000356397.1
• Coordinates: 4461274..4461564 (+)
• Length: 97 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CRT34_RS22325	4456876..4457475	+	600	WP_001296610.1	glucose-1-phosphatase	-
CRT34_RS22330	4457469..4458341	+	873	WP_000920762.1	virulence factor BrkB family protein	-
CRT34_RS22335	4458338..4458775	+	438	WP_000560981.1	D-tyrosyl-tRNA(Tyr) deacylase	-
CRT34_RS22340	4458820..4459761	+	942	WP_001297068.1	fatty acid biosynthesis protein FabY	-
CRT34_RS22345	4459825..4460733	-	909	WP_001385591.1	alpha/beta hydrolase	-
CRT34_RS22350	4460962..4461273	+	312	WP_000897302.1	hypothetical protein	Toxin
CRT34_RS22355	4461274..4461564	+	291	WP_000356397.1	helix-turn-helix domain-containing protein	Antitoxin
CRT34_RS22360	4461649..4461861	+	213	WP_000197774.1	hypothetical protein	-
CRT34_RS22365	4461923..4462201	+	279	WP_001296612.1	hypothetical protein	-
CRT34_RS22375	4462598..4462816	+	219	WP_001251293.1	ribbon-helix-helix domain-containing protein	-
CRT34_RS22380	4463001..4463450	-	450	WP_032140890.1	hypothetical protein	-
CRT34_RS22385	4463766..4464614	-	849	WP_001038650.1	hypothetical protein	-
CRT34_RS22390	4464904..4465146	+	243	WP_001068514.1	ribbon-helix-helix domain-containing protein	-
CRT34_RS22395	4465328..4466257	-	930	WP_000027696.1	formate dehydrogenase accessory protein FdhE	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

No domain identified.

Antitoxin

• Cro/C1-type helix-turn-helix domain (36-85)

Sequences

Toxin

Protein

• Length: 104 a.a.
• Molecular weight: 12203.19 Da
• Isoelectric Point: 9.7791

>T86253 WP_000897302.1 NZ_CP023849:4460962-4461273 [Escherichia coli]
MLFIETEIFTEDVQKLLNDDEFSRFQFFLALNPDYGEVIPETGGLRKVRWVSGGKGKRAGVRVIYFHQVKHYEIRLLLIY
RKGIKDDLSPQEKAMLRLLNTRW

Nucleotide

• Length: 312 bp

>T86253 NZ_CP023849:4460962-4461273 [Escherichia coli]
ATGCTCTTCATCGAAACGGAAATTTTTACTGAAGATGTTCAAAAACTGCTGAACGACGATGAATTCAGTCGTTTTCAGTT
TTTCCTGGCCTTGAATCCTGACTATGGTGAGGTAATACCTGAAACTGGTGGGTTAAGAAAGGTTCGATGGGTCTCAGGAG
GAAAGGGGAAACGTGCTGGTGTCAGGGTTATTTACTTTCATCAGGTTAAACATTATGAGATCAGATTACTGCTTATTTAT
CGTAAGGGTATTAAAGATGATCTGTCTCCCCAGGAAAAAGCAATGCTCCGGTTGTTAAATACGAGGTGGTAA

Antitoxin

Protein

• Length: 97 a.a.
• Molecular weight: 10846.43 Da
• Isoelectric Point: 10.8907

>AT86253 WP_000356397.1 NZ_CP023849:4461274-4461564 [Escherichia coli]
MDKALFERLTHSMAQMNEIIEGTRQPSRTFEVDAMKIKEIRRASGLSQSKFADLISVSVDTLRNWEQGRRSPTGPAKALL
RAIANDPQHVLQALNR

Nucleotide

• Length: 291 bp

>AT86253 NZ_CP023849:4461274-4461564 [Escherichia coli]
ATGGATAAGGCACTTTTTGAGCGGTTGACTCACAGCATGGCCCAGATGAATGAAATTATTGAAGGAACGCGACAGCCTTC
ACGGACTTTTGAAGTTGATGCTATGAAAATCAAAGAAATACGCCGCGCTTCAGGGTTGTCACAATCTAAATTTGCCGATC
TTATTTCCGTAAGCGTAGACACGTTGCGTAACTGGGAGCAGGGGCGACGTTCACCGACCGGGCCTGCAAAGGCTTTGCTG
CGAGCGATTGCCAATGATCCGCAACATGTGCTGCAAGCACTTAACCGTTAG

Structures

Toxin

• AlphaFold DB: S1P416