Detailed information of TA system TA086251

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): prlF-yhaV (relBE)/YhaV-PrlF
• Location: 3650483..3651282
• Replicon: chromosome
• Accession: NZ_CP023849
• Organism: Escherichia coli strain 4/0

Toxin (Protein)

• Gene name: yhaV
• Uniprot ID: S1NYM6
• Locus tag: CRT34_RS18370
• Protein ID: WP_000347251.1
• Coordinates: 3650818..3651282 (+)
• Length: 155 a.a.

Antitoxin (Protein)

• Gene name: prlF
• Uniprot ID: S1PPV5
• Locus tag: CRT34_RS18365
• Protein ID: WP_001296435.1
• Coordinates: 3650483..3650818 (+)
• Length: 112 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CRT34_RS18350	3646268..3647038	-	771	WP_001058227.1	2-dehydro-3-deoxyglucarate aldolase	-
CRT34_RS18355	3647054..3648388	-	1335	WP_000599651.1	galactarate/glucarate/glycerate transporter GarP	-
CRT34_RS18360	3648763..3650334	+	1572	WP_001273763.1	galactarate dehydratase	-
CRT34_RS18365	3650483..3650818	+	336	WP_001296435.1	type II toxin-antitoxin system antitoxin PrlF	Antitoxin
CRT34_RS18370	3650818..3651282	+	465	WP_000347251.1	type II toxin-antitoxin system ribonuclease toxin YhaV	Toxin
CRT34_RS18375	3651337..3652146	-	810	WP_000072187.1	aga operon transcriptional regulator AgaR	-
CRT34_RS18380	3652395..3653675	+	1281	WP_000681950.1	tagatose-bisphosphate aldolase subunit KbaZ	-
CRT34_RS18385	3653698..3654171	+	474	WP_001295547.1	PTS N-acetylgalactosamine transporter subunit IIB	-
CRT34_RS18390	3654182..3654961	+	780	WP_000406214.1	PTS mannose/fructose/sorbose/N-acetylgalactosamine transporter subunit IIC	-
CRT34_RS18395	3654951..3655829	+	879	WP_001295548.1	PTS system mannose/fructose/sorbose family transporter subunit IID	-
CRT34_RS18400	3655847..3656281	+	435	WP_000948824.1	PTS sugar transporter subunit IIA	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin endonuclease, YhaV (12-150)

Antitoxin

• PrlF antitoxin (14-110)

Sequences

Toxin

Protein

• Length: 155 a.a.
• Molecular weight: 17777.14 Da
• Isoelectric Point: 9.4947

>T86251 WP_000347251.1 NZ_CP023849:3650818-3651282 [Escherichia coli]
MDFPQRVNGWALYAHPCFQETYDALVAEVEALKGKDPENYQRKAATKLLAVVHKVIEEHITVNPSSPAFRHGKSLGSGKN
KDWSRVKFGAGRYRLFFRYSEKEKVIILGWMNDENTLRTYGKKTDAYTVFSKMLKRGHPPADWESLTQETEENH

Nucleotide

• Length: 465 bp

>T86251 NZ_CP023849:3650818-3651282 [Escherichia coli]
ATGGATTTTCCACAAAGGGTTAATGGTTGGGCGCTATATGCTCATCCCTGTTTTCAGGAAACCTACGATGCTTTAGTTGC
CGAAGTCGAGGCATTAAAGGGAAAAGATCCTGAAAATTATCAGAGAAAAGCTGCCACAAAGTTATTGGCGGTGGTCCATA
AAGTGATTGAGGAGCATATCACGGTCAATCCATCATCACCGGCATTCCGTCATGGCAAGTCGTTAGGCTCTGGGAAAAAT
AAAGACTGGTCACGGGTAAAATTTGGTGCTGGTCGCTATCGTCTCTTCTTTCGTTACAGTGAAAAAGAGAAAGTCATCAT
TCTGGGATGGATGAACGATGAAAACACGCTGCGCACCTACGGTAAAAAAACAGATGCCTATACCGTATTCAGTAAAATGT
TAAAAAGAGGACATCCTCCTGCCGACTGGGAAAGCCTCACCCAAGAGACAGAAGAAAACCATTGA

Antitoxin

Protein

• Length: 112 a.a.
• Molecular weight: 12321.92 Da
• Isoelectric Point: 4.5392

>AT86251 WP_001296435.1 NZ_CP023849:3650483-3650818 [Escherichia coli]
MPANARSNAVLTTESKVTIRGQTTIPAPVREALKLKPGLDSIHYEILPGGQVFMCRLGDEQEDHTMNAFLRFLDADIQNN
PQKTRPFDIQQGKKLVAGMDVNIDDEIGDDE

Nucleotide

• Length: 336 bp

>AT86251 NZ_CP023849:3650483-3650818 [Escherichia coli]
ATGCCCGCTAATGCTCGCTCTAACGCTGTACTGACCACTGAATCAAAGGTCACTATACGCGGACAAACAACTATCCCCGC
GCCAGTGCGTGAGGCCTTAAAACTGAAGCCAGGCCTGGACAGCATTCATTACGAAATTCTGCCTGGTGGGCAAGTATTTA
TGTGCCGACTGGGAGATGAACAGGAGGATCATACTATGAATGCATTTTTGCGTTTTCTGGATGCAGATATCCAGAACAAT
CCGCAAAAAACTCGTCCATTCGACATTCAACAAGGAAAGAAACTTGTCGCTGGCATGGACGTCAACATTGACGATGAGAT
TGGCGACGACGAATAA

Structures

Toxin

• AlphaFold DB: A0A829CJ20

Antitoxin

• AlphaFold DB: S1PPV5