Detailed information of TA system TA086224

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 142938..143359
• Replicon: plasmid p4_1_1.1
• Accession: NZ_CP023845
• Organism: Escherichia coli strain 4/1-1

Toxin (Protein)

• Gene name: hok
• Uniprot ID: B1VC78
• Locus tag: CRT35_RS26600
• Protein ID: WP_001302184.1
• Coordinates: 143201..143359 (+)
• Length: 53 a.a.

Antitoxin (RNA)

Gene name	srnC
Locus tag	-
Coordinates	142938..143136 (+)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CRT35_RS26560	138634..140028	+	1395	WP_001037799.1	carbohydrate porin	-
CRT35_RS26570	140274..140378	-	105	Protein_157	thiosulfate reductase cytochrome B subunit	-
CRT35_RS26575	140435..141128	+	694	Protein_158	IS1 family transposase	-
CRT35_RS26580	141144..141767	+	624	WP_000912556.1	potassium channel family protein	-
CRT35_RS26585	141852..142103	+	252	Protein_160	iron-containing alcohol dehydrogenase	-
CRT35_RS26590	142392..142709	-	318	Protein_161	IS110 family transposase	-
CRT35_RS26595	142864..142969	+	106	Protein_162	plasmid SOS inhibition protein A	-
-	142938..143136	+	199	NuclAT_0	-	Antitoxin
-	142938..143136	+	199	NuclAT_0	-	Antitoxin
-	142938..143136	+	199	NuclAT_0	-	Antitoxin
-	142938..143136	+	199	NuclAT_0	-	Antitoxin
CRT35_RS26600	143201..143359	+	159	WP_001302184.1	type I toxin-antitoxin system Hok family toxin	Toxin
CRT35_RS27460	143732..144025	+	294	WP_175271036.1	hypothetical protein	-
CRT35_RS27465	144027..144233	+	207	WP_000275854.1	hypothetical protein	-
CRT35_RS26610	144258..144545	+	288	WP_000107242.1	hypothetical protein	-
CRT35_RS26615	144664..145485	+	822	WP_001234465.1	DUF945 domain-containing protein	-
CRT35_RS26620	145781..146353	-	573	WP_000949660.1	transglycosylase SLT domain-containing protein	-
CRT35_RS26625	146723..147106	+	384	WP_001063020.1	relaxosome protein TraM	-
CRT35_RS26630	147297..147944	+	648	WP_000332520.1	conjugal transfer transcriptional regulator TraJ	-
CRT35_RS26635	148080..148295	+	216	WP_001352842.1	conjugal transfer relaxosome protein TraY	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	tet(A) / aac(6')-Ib-cr / blaOXA-1 / blaCTX-M-15 / blaTEM-1B / aph(3')-Ia / mph(A) / sul1 / qacE / aadA2 / dfrA12	-	1..181324	181324

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (8-48)

Sequences

Toxin

Protein

• Length: 53 a.a.
• Molecular weight: 6108.35 Da
• Isoelectric Point: 9.1977

>T86224 WP_001302184.1 NZ_CP023845:143201-143359 [Escherichia coli]
MKLPRSSLVWCVLIVCLTLLIFTYLTRKSLCEIRYRDGYREVAAFMAYESGK

Nucleotide

• Length: 159 bp

>T86224 NZ_CP023845:143201-143359 [Escherichia coli]
ATGAAACTACCACGCAGCTCTCTTGTCTGGTGTGTGTTGATCGTGTGTCTCACACTGTTGATATTCACTTATCTGACACG
AAAATCGCTGTGCGAGATTCGTTACAGAGACGGATACAGGGAGGTGGCGGCTTTCATGGCTTACGAATCCGGTAAGTAG

Antitoxin

Nucleotide

• Length: 199 bp

>AT86224 NZ_CP023845:142938-143136 [Escherichia coli]
TCACACAGATTACCCGTAAACAGTCTGAATGAGCGGGTTATTTTCAGGAAAAGTGAGTGTGGTCAGCGTGCAGGGATATG
GGCTATGATGTGCCCGGCGCTTGAGGCTTTCTGCCTCATGACGTGAAGGTGGTTTGTTACCGTGTTGTGTGGCAGAAGGA
CAAAAGCCCCGTAGTTAATTTTTCATTAACCCACGAGGC

Structures

Antitoxin