Detailed information of TA system TA086164

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 61281..61535
• Replicon: plasmid p4_2_1.1
• Accession: NZ_CP023835
• Organism: Escherichia coli strain 4/2-1

Toxin (Protein)

• Gene name: srnB
• Uniprot ID: -
• Locus tag: CRT37_RS23220
• Protein ID: WP_015387399.1
• Coordinates: 61329..61535 (+)
• Length: 69 a.a.

Antitoxin (RNA)

Gene name	sok
Locus tag	-
Coordinates	61281..61342 (-)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CRT37_RS23175	56933..57145	+	213	WP_005012601.1	hypothetical protein	-
CRT37_RS24390	57446..57535	-	90	Protein_67	IS1 family transposase	-
CRT37_RS23190	57590..58267	+	678	WP_001339397.1	IS66 family insertion sequence hypothetical protein	-
CRT37_RS23195	58267..58614	+	348	WP_000624622.1	IS66 family insertion sequence element accessory protein TnpB	-
CRT37_RS23200	58634..60205	+	1572	WP_023149734.1	IS66 family transposase	-
CRT37_RS23205	60243..60859	-	617	Protein_71	IS1 family transposase	-
CRT37_RS23210	60960..61142	+	183	WP_000968309.1	hypothetical protein	-
-	61281..61342	-	62	NuclAT_2	-	Antitoxin
-	61281..61342	-	62	NuclAT_2	-	Antitoxin
-	61281..61342	-	62	NuclAT_2	-	Antitoxin
-	61281..61342	-	62	NuclAT_2	-	Antitoxin
CRT37_RS23220	61329..61535	+	207	WP_015387399.1	type I toxin-antitoxin system Hok family toxin	Toxin
CRT37_RS23225	61819..62076	+	258	WP_000083833.1	replication regulatory protein RepA	-
CRT37_RS23235	62312..62386	+	75	WP_032336874.1	RepA leader peptide Tap	-
CRT37_RS23240	62379..62825	+	447	Protein_76	incFII family plasmid replication initiator RepA	-
CRT37_RS23245	62771..63253	-	483	Protein_77	VENN motif pre-toxin domain-containing protein	-
CRT37_RS23250	64146..65366	+	1221	WP_000410951.1	arginine deiminase	-
CRT37_RS23255	65377..66288	+	912	WP_000440183.1	carbamate kinase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	sul1 / qacE / aadA5 / aac(6')-Ib-cr / blaOXA-1 / aac(3)-IIa / blaCTX-M-15 / sitABCD	iucA / iucB / iucC / iucD / iutA	1..152425	152425

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (23-64)

Sequences

Toxin

Protein

• Length: 69 a.a.
• Molecular weight: 7720.23 Da
• Isoelectric Point: 9.3127

>T86164 WP_015387399.1 NZ_CP023835:61329-61535 [Escherichia coli]
MKYLNTTDCSLFLAGRSKFMTKYALIGLLAVCATVLCFSLIFRERLCELNIHRGNTVVQVTLAYEARK

Nucleotide

• Length: 207 bp

>T86164 NZ_CP023835:61329-61535 [Escherichia coli]
ATGAAGTACCTTAACACTACTGATTGTAGCCTCTTCCTTGCAGGGAGGTCAAAGTTTATGACGAAATATGCCCTTATCGG
GTTGCTCGCCGTGTGCGCCACGGTGTTGTGTTTTTCACTGATATTCAGGGAACGGTTATGTGAACTGAATATTCACAGGG
GAAATACAGTGGTGCAGGTAACTCTGGCCTACGAAGCACGGAAGTAA

Antitoxin

Nucleotide

• Length: 62 bp

>AT86164 NZ_CP023835:c61342-61281 [Escherichia coli]
TTAAGGTACTTCATGCAGACCTCACGAGTTAATGGATTAACGAGTGGGGTCTTCGCATTTCT

Structures

Antitoxin