Detailed information of TA system TA086160

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 19920..20153
• Replicon: plasmid p4_2_1.1
• Accession: NZ_CP023835
• Organism: Escherichia coli strain 4/2-1

Toxin (Protein)

• Gene name: hok
• Uniprot ID: P11895
• Locus tag: CRT37_RS22965
• Protein ID: WP_001312861.1
• Coordinates: 19995..20153 (+)
• Length: 53 a.a.

Antitoxin (RNA)

Gene name	sok
Locus tag	-
Coordinates	19920..19951 (+)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CRT37_RS22935	16041..16538	+	498	WP_042039995.1	single-stranded DNA-binding protein	-
CRT37_RS22940	16606..16839	+	234	WP_000005990.1	DUF905 family protein	-
CRT37_RS22945	16867..17064	+	198	Protein_21	hypothetical protein	-
CRT37_RS22950	17119..17553	+	435	WP_000845937.1	conjugation system SOS inhibitor PsiB	-
CRT37_RS22955	17550..18269	+	720	WP_001276238.1	plasmid SOS inhibition protein A	-
CRT37_RS24280	18281..18469	-	189	WP_001299721.1	hypothetical protein	-
-	18281..18478	+	198	NuclAT_0	-	-
-	18281..18478	+	198	NuclAT_0	-	-
-	18281..18478	+	198	NuclAT_0	-	-
-	18281..18478	+	198	NuclAT_0	-	-
CRT37_RS22960	18526..19895	+	1370	WP_085947770.1	IS3-like element IS150 family transposase	-
-	19920..19951	+	32	NuclAT_1	-	Antitoxin
-	19920..19951	+	32	NuclAT_1	-	Antitoxin
-	19920..19951	+	32	NuclAT_1	-	Antitoxin
-	19920..19951	+	32	NuclAT_1	-	Antitoxin
CRT37_RS22965	19995..20153	+	159	WP_001312861.1	type I toxin-antitoxin system Hok family toxin	Toxin
CRT37_RS22980	21074..21361	+	288	WP_000107535.1	hypothetical protein	-
CRT37_RS22985	21479..22300	+	822	WP_001234426.1	DUF945 domain-containing protein	-
CRT37_RS22990	22597..23199	-	603	WP_013362798.1	transglycosylase SLT domain-containing protein	-
CRT37_RS22995	23520..23903	+	384	WP_001151524.1	relaxosome protein TraM	-
CRT37_RS23000	24090..24779	+	690	WP_000283385.1	conjugal transfer transcriptional regulator TraJ	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	sul1 / qacE / aadA5 / aac(6')-Ib-cr / blaOXA-1 / aac(3)-IIa / blaCTX-M-15 / sitABCD	iucA / iucB / iucC / iucD / iutA	1..152425	152425

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (8-48)

Sequences

Toxin

Protein

• Length: 53 a.a.
• Molecular weight: 6082.32 Da
• Isoelectric Point: 9.2584

>T86160 WP_001312861.1 NZ_CP023835:19995-20153 [Escherichia coli]
MKLPRSSLVWCVLIVCLTLLIFTYLTRKSLCEIRYRDGHREVAAFMAYESGK

Nucleotide

• Length: 159 bp

>T86160 NZ_CP023835:19995-20153 [Escherichia coli]
ATGAAACTACCACGAAGTTCCCTTGTCTGGTGTGTGTTGATCGTGTGTCTCACACTGTTGATATTCACTTATCTGACACG
AAAATCGCTGTGCGAGATTCGTTACAGAGACGGACACAGGGAGGTGGCGGCTTTCATGGCTTACGAATCCGGTAAGTAG

Antitoxin

Nucleotide

• Length: 32 bp

>AT86160 NZ_CP023835:19920-19951 [Escherichia coli]
CACCACGAGGCATCCCTATGTCTAGTCCACAT

Structures

Toxin

• AlphaFold DB: P11895

Antitoxin