Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) yefM-yoeB (relBE)/YoeB-YefM
Location 2141348..2141850 Replicon chromosome
Accession NZ_CP023834
Organism Escherichia coli strain 4/2-1

Toxin (Protein)


Gene name yoeB Uniprot ID U9YDH0
Locus tag CRT37_RS10380 Protein ID WP_000767829.1
Coordinates 2141348..2141602 (-) Length 85 a.a.

Antitoxin (Protein)


Gene name yefM Uniprot ID S1Q063
Locus tag CRT37_RS10385 Protein ID WP_001259255.1
Coordinates 2141599..2141850 (-) Length 84 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
CRT37_RS10350 2136363..2136590 - 228 WP_000985273.1 sulfurtransferase TusA family protein -
CRT37_RS10355 2136604..2137662 - 1059 WP_000492333.1 transport protein YeeE -
CRT37_RS10360 2137841..2139199 - 1359 WP_000019197.1 putrescine/proton symporter PlaP -
CRT37_RS10365 2139189..2139251 - 63 WP_010723108.1 membrane protein YoeI -
CRT37_RS10370 2139466..2140395 - 930 WP_000803351.1 LysR family transcriptional regulator -
CRT37_RS10375 2140441..2141265 - 825 WP_000754737.1 SDR family oxidoreductase -
CRT37_RS10380 2141348..2141602 - 255 WP_000767829.1 type II toxin-antitoxin system mRNA interferase toxin YoeB Toxin
CRT37_RS10385 2141599..2141850 - 252 WP_001259255.1 YoeB-YefM toxin-antitoxin system antitoxin YefM Antitoxin
CRT37_RS10395 2142133..2142183 + 51 WP_001364200.1 his operon leader peptide -
CRT37_RS10400 2142329..2143228 + 900 WP_000131792.1 ATP phosphoribosyltransferase -
CRT37_RS10405 2143234..2144538 + 1305 WP_042345632.1 histidinol dehydrogenase -
CRT37_RS10410 2144535..2145605 + 1071 WP_000108991.1 histidinol-phosphate transaminase -
CRT37_RS10415 2145605..2146672 + 1068 WP_000080064.1 bifunctional histidinol-phosphatase/imidazoleglycerol-phosphate dehydratase HisB -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin YoeB

(2-84)

Antitoxin

Type II toxin-antitoxin system, antitoxin Phd/YefM

(1-73)


Sequences

Toxin        


Download         Length: 85 a.a.        Molecular weight: 10183.58 Da        Isoelectric Point: 8.0353

>T86151 WP_000767829.1 NZ_CP023834:c2141602-2141348 [Escherichia coli]
VKLIWSEESWDDYLYWQETDKRIVKKINELIKDTRRTPFEGKGKPEPLKHNLSGFWSRRITEEHRLVYAVTDDSLLIAAC
RYHY

Download         Length: 255 bp

>T86151 NZ_CP023834:c2141602-2141348 [Escherichia coli]
GTGAAACTAATCTGGTCTGAGGAATCATGGGATGATTATCTGTACTGGCAGGAAACAGATAAGCGAATTGTTAAAAAGAT
CAATGAACTTATCAAAGATACCCGCAGAACACCATTTGAAGGTAAGGGGAAACCAGAACCCCTGAAACATAATTTGTCAG
GTTTCTGGTCCCGACGCATTACAGAGGAGCACCGTCTGGTATACGCGGTTACTGATGATTCACTGCTCATTGCAGCATGT
CGTTATCATTATTGA

Antitoxin


Download         Length: 84 a.a.        Molecular weight: 9307.58 Da        Isoelectric Point: 4.7954

>AT86151 WP_001259255.1 NZ_CP023834:c2141850-2141599 [Escherichia coli]
MRTISYSEARQNLSATMMKAVEDHAPILITRQNGEACVLMSLEEYNSLEETAYLLRSPANARRLMDSIDSLKSGKGTEKD
IIE

Download         Length: 252 bp

>AT86151 NZ_CP023834:c2141850-2141599 [Escherichia coli]
ATGCGTACAATTAGCTACAGCGAAGCGCGTCAGAATTTGTCGGCAACAATGATGAAAGCCGTTGAAGATCATGCCCCGAT
CCTCATTACTCGTCAGAATGGAGAGGCTTGTGTTCTGATGTCACTCGAAGAATACAACTCGCTGGAAGAGACGGCTTATC
TACTGCGTTCCCCCGCTAACGCCCGGAGATTGATGGACTCAATCGATAGCCTGAAATCAGGCAAAGGAACGGAAAAGGAC
ATTATTGAGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 6NY6
PDB 2A6S
PDB 6OXA
PDB 2A6R
PDB 4V8X
PDB 6OTR
PDB 6OXI
PDB 2A6Q

Antitoxin

Source ID Structure
PDB 2A6Q
AlphaFold DB A0A7U9LNR0

References