86151

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) yefM-yoeB (relBE)/YoeB-YefM
Location 2141348..2141850 Replicon chromosome
Accession NZ_CP023834
Organism Escherichia coli strain 4/2-1

Toxin (Protein)


Gene name yoeB Uniprot ID U9YDH0
Locus tag CRT37_RS10380 Protein ID WP_000767829.1
Coordinates 2141348..2141602 (-) Length 85 a.a.

Antitoxin (Protein)


Gene name yefM Uniprot ID S1Q063
Locus tag CRT37_RS10385 Protein ID WP_001259255.1
Coordinates 2141599..2141850 (-) Length 84 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
CRT37_RS10350 2136363..2136590 - 228 WP_000985273.1 sulfurtransferase TusA family protein -
CRT37_RS10355 2136604..2137662 - 1059 WP_000492333.1 transport protein YeeE -
CRT37_RS10360 2137841..2139199 - 1359 WP_000019197.1 putrescine/proton symporter PlaP -
CRT37_RS10365 2139189..2139251 - 63 WP_010723108.1 membrane protein YoeI -
CRT37_RS10370 2139466..2140395 - 930 WP_000803351.1 LysR family transcriptional regulator -
CRT37_RS10375 2140441..2141265 - 825 WP_000754737.1 SDR family oxidoreductase -
CRT37_RS10380 2141348..2141602 - 255 WP_000767829.1 type II toxin-antitoxin system mRNA interferase toxin YoeB Toxin
CRT37_RS10385 2141599..2141850 - 252 WP_001259255.1 YoeB-YefM toxin-antitoxin system antitoxin YefM Antitoxin
CRT37_RS10395 2142133..2142183 + 51 WP_001364200.1 his operon leader peptide -
CRT37_RS10400 2142329..2143228 + 900 WP_000131792.1 ATP phosphoribosyltransferase -
CRT37_RS10405 2143234..2144538 + 1305 WP_042345632.1 histidinol dehydrogenase -
CRT37_RS10410 2144535..2145605 + 1071 WP_000108991.1 histidinol-phosphate transaminase -
CRT37_RS10415 2145605..2146672 + 1068 WP_000080064.1 bifunctional histidinol-phosphatase/imidazoleglycerol-phosphate dehydratase HisB -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin YoeB

(2-84)

Antitoxin

Type II toxin-antitoxin system, antitoxin Phd/YefM

(1-73)


Sequences

Toxin        


Download         Length: 85 a.a.        Molecular weight: 10183.58 Da        Isoelectric Point: 8.0353

>T86151 WP_000767829.1 NZ_CP023834:c2141602-2141348 [Escherichia coli]
VKLIWSEESWDDYLYWQETDKRIVKKINELIKDTRRTPFEGKGKPEPLKHNLSGFWSRRITEEHRLVYAVTDDSLLIAAC
RYHY

Download         Length: 255 bp

>T86151 NZ_CP023834:c2141602-2141348 [Escherichia coli]
GTGAAACTAATCTGGTCTGAGGAATCATGGGATGATTATCTGTACTGGCAGGAAACAGATAAGCGAATTGTTAAAAAGAT
CAATGAACTTATCAAAGATACCCGCAGAACACCATTTGAAGGTAAGGGGAAACCAGAACCCCTGAAACATAATTTGTCAG
GTTTCTGGTCCCGACGCATTACAGAGGAGCACCGTCTGGTATACGCGGTTACTGATGATTCACTGCTCATTGCAGCATGT
CGTTATCATTATTGA

Antitoxin


Download         Length: 84 a.a.        Molecular weight: 9307.58 Da        Isoelectric Point: 4.7954

>AT86151 WP_001259255.1 NZ_CP023834:c2141850-2141599 [Escherichia coli]
MRTISYSEARQNLSATMMKAVEDHAPILITRQNGEACVLMSLEEYNSLEETAYLLRSPANARRLMDSIDSLKSGKGTEKD
IIE

Download         Length: 252 bp

>AT86151 NZ_CP023834:c2141850-2141599 [Escherichia coli]
ATGCGTACAATTAGCTACAGCGAAGCGCGTCAGAATTTGTCGGCAACAATGATGAAAGCCGTTGAAGATCATGCCCCGAT
CCTCATTACTCGTCAGAATGGAGAGGCTTGTGTTCTGATGTCACTCGAAGAATACAACTCGCTGGAAGAGACGGCTTATC
TACTGCGTTCCCCCGCTAACGCCCGGAGATTGATGGACTCAATCGATAGCCTGAAATCAGGCAAAGGAACGGAAAAGGAC
ATTATTGAGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 6NY6
PDB 2A6S
PDB 6OXA
PDB 2A6R
PDB 4V8X
PDB 6OTR
PDB 6OXI
PDB 2A6Q

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9LNR0

References