Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) prlF-yhaV (relBE)/YhaV-PrlF
Location 3633938..3634737 Replicon chromosome
Accession NZ_CP023826
Organism Escherichia coli strain 4/4

Toxin (Protein)


Gene name yhaV Uniprot ID S1NYM6
Locus tag CRT43_RS18275 Protein ID WP_000347251.1
Coordinates 3634273..3634737 (+) Length 155 a.a.

Antitoxin (Protein)


Gene name prlF Uniprot ID S1PPV5
Locus tag CRT43_RS18270 Protein ID WP_001296435.1
Coordinates 3633938..3634273 (+) Length 112 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
CRT43_RS18255 3629723..3630493 - 771 WP_001058227.1 2-dehydro-3-deoxyglucarate aldolase -
CRT43_RS18260 3630509..3631843 - 1335 WP_000599651.1 galactarate/glucarate/glycerate transporter GarP -
CRT43_RS18265 3632218..3633789 + 1572 WP_001273763.1 galactarate dehydratase -
CRT43_RS18270 3633938..3634273 + 336 WP_001296435.1 type II toxin-antitoxin system antitoxin PrlF Antitoxin
CRT43_RS18275 3634273..3634737 + 465 WP_000347251.1 type II toxin-antitoxin system ribonuclease toxin YhaV Toxin
CRT43_RS18280 3634792..3635601 - 810 WP_000072187.1 aga operon transcriptional regulator AgaR -
CRT43_RS18285 3635850..3637130 + 1281 WP_000681950.1 tagatose-bisphosphate aldolase subunit KbaZ -
CRT43_RS18290 3637153..3637626 + 474 WP_001295547.1 PTS N-acetylgalactosamine transporter subunit IIB -
CRT43_RS18295 3637637..3638416 + 780 WP_000406214.1 PTS mannose/fructose/sorbose/N-acetylgalactosamine transporter subunit IIC -
CRT43_RS18300 3638406..3639284 + 879 WP_001295548.1 PTS system mannose/fructose/sorbose family transporter subunit IID -
CRT43_RS18305 3639302..3639736 + 435 WP_000948824.1 PTS sugar transporter subunit IIA -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin endonuclease, YhaV

(12-150)

Antitoxin

PrlF antitoxin

(14-110)


Sequences

Toxin        


Download         Length: 155 a.a.        Molecular weight: 17777.14 Da        Isoelectric Point: 9.4947

>T86123 WP_000347251.1 NZ_CP023826:3634273-3634737 [Escherichia coli]
MDFPQRVNGWALYAHPCFQETYDALVAEVEALKGKDPENYQRKAATKLLAVVHKVIEEHITVNPSSPAFRHGKSLGSGKN
KDWSRVKFGAGRYRLFFRYSEKEKVIILGWMNDENTLRTYGKKTDAYTVFSKMLKRGHPPADWESLTQETEENH

Download         Length: 465 bp

>T86123 NZ_CP023826:3634273-3634737 [Escherichia coli]
ATGGATTTTCCACAAAGGGTTAATGGTTGGGCGCTATATGCTCATCCCTGTTTTCAGGAAACCTACGATGCTTTAGTTGC
CGAAGTCGAGGCATTAAAGGGAAAAGATCCTGAAAATTATCAGAGAAAAGCTGCCACAAAGTTATTGGCGGTGGTCCATA
AAGTGATTGAGGAGCATATCACGGTCAATCCATCATCACCGGCATTCCGTCATGGCAAGTCGTTAGGCTCTGGGAAAAAT
AAAGACTGGTCACGGGTAAAATTTGGTGCTGGTCGCTATCGTCTCTTCTTTCGTTACAGTGAAAAAGAGAAAGTCATCAT
TCTGGGATGGATGAACGATGAAAACACGCTGCGCACCTACGGTAAAAAAACAGATGCCTATACCGTATTCAGTAAAATGT
TAAAAAGAGGACATCCTCCTGCCGACTGGGAAAGCCTCACCCAAGAGACAGAAGAAAACCATTGA

Antitoxin


Download         Length: 112 a.a.        Molecular weight: 12321.92 Da        Isoelectric Point: 4.5392

>AT86123 WP_001296435.1 NZ_CP023826:3633938-3634273 [Escherichia coli]
MPANARSNAVLTTESKVTIRGQTTIPAPVREALKLKPGLDSIHYEILPGGQVFMCRLGDEQEDHTMNAFLRFLDADIQNN
PQKTRPFDIQQGKKLVAGMDVNIDDEIGDDE

Download         Length: 336 bp

>AT86123 NZ_CP023826:3633938-3634273 [Escherichia coli]
ATGCCCGCTAATGCTCGCTCTAACGCTGTACTGACCACTGAATCAAAGGTCACTATACGCGGACAAACAACTATCCCCGC
GCCAGTGCGTGAGGCCTTAAAACTGAAGCCAGGCCTGGACAGCATTCATTACGAAATTCTGCCTGGTGGGCAAGTATTTA
TGTGCCGACTGGGAGATGAACAGGAGGATCATACTATGAATGCATTTTTGCGTTTTCTGGATGCAGATATCCAGAACAAT
CCGCAAAAAACTCGTCCATTCGACATTCAACAAGGAAAGAAACTTGTCGCTGGCATGGACGTCAACATTGACGATGAGAT
TGGCGACGACGAATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A829CJ20

Antitoxin

Source ID Structure
AlphaFold DB S1PPV5

References