Detailed information of TA system TA086119

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): yefM-yoeB (relBE)/YoeB-YefM
• Location: 2274916..2275418
• Replicon: chromosome
• Accession: NZ_CP023826
• Organism: Escherichia coli strain 4/4

Toxin (Protein)

• Gene name: yoeB
• Uniprot ID: E2QNP2
• Locus tag: CRT43_RS11805
• Protein ID: WP_000767819.1
• Coordinates: 2274916..2275170 (-)
• Length: 85 a.a.

Antitoxin (Protein)

• Gene name: yefM
• Uniprot ID: E2QNP3
• Locus tag: CRT43_RS11810
• Protein ID: WP_001259253.1
• Coordinates: 2275167..2275418 (-)
• Length: 84 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CRT43_RS11775	2270734..2271192	-	459	WP_001531805.1	IS200/IS605-like element IS200C family transposase	-
CRT43_RS11785	2271409..2272767	-	1359	WP_000019194.1	putrescine/proton symporter PlaP	-
CRT43_RS11790	2272757..2272819	-	63	WP_010723108.1	membrane protein YoeI	-
CRT43_RS11795	2273034..2273963	-	930	WP_000803366.1	LysR family transcriptional regulator	-
CRT43_RS11800	2274009..2274833	-	825	WP_000754737.1	SDR family oxidoreductase	-
CRT43_RS11805	2274916..2275170	-	255	WP_000767819.1	type II toxin-antitoxin system mRNA interferase toxin YoeB	Toxin
CRT43_RS11810	2275167..2275418	-	252	WP_001259253.1	YoeB-YefM toxin-antitoxin system antitoxin YefM	Antitoxin
CRT43_RS11820	2275701..2275751	+	51	WP_001364200.1	his operon leader peptide	-
CRT43_RS11825	2275897..2276796	+	900	WP_000131782.1	ATP phosphoribosyltransferase	-
CRT43_RS11830	2276802..2278106	+	1305	WP_001296211.1	histidinol dehydrogenase	-
CRT43_RS11835	2278103..2279173	+	1071	WP_000108967.1	histidinol-phosphate transaminase	-
CRT43_RS11840	2279173..2280240	+	1068	WP_000080057.1	bifunctional histidinol-phosphatase/imidazoleglycerol-phosphate dehydratase HisB	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	flank	IS/Tn	-	-	2270734..2271192	458

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin YoeB (2-84)

Antitoxin

• Type II toxin-antitoxin system, antitoxin Phd/YefM (1-72)

Sequences

Toxin

Protein

• Length: 85 a.a.
• Molecular weight: 10110.53 Da
• Isoelectric Point: 7.2749

>T86119 WP_000767819.1 NZ_CP023826:c2275170-2274916 [Escherichia coli]
VKLIWSEESWDDYLYWQEADKRIVKKINELIKDTRRTPFEGKGKPEPLKHNLSGFWSRRITEEHLLVYAVTDDSLLIAAC
RYHY

Nucleotide

• Length: 255 bp

>T86119 NZ_CP023826:c2275170-2274916 [Escherichia coli]
GTGAAACTAATCTGGTCTGAGGAATCATGGGATGATTATCTGTACTGGCAGGAAGCAGATAAGCGAATTGTTAAAAAGAT
CAATGAACTTATCAAAGATACCCGCAGAACACCATTTGAAGGTAAGGGGAAACCAGAACCCCTGAAACATAACTTGTCAG
GTTTCTGGTCCCGACGCATTACAGAGGAGCACCTTCTGGTATACGCGGTTACTGATGATTCACTGCTCATTGCAGCATGT
CGTTATCATTATTGA

Antitoxin

Protein

• Length: 84 a.a.
• Molecular weight: 9365.62 Da
• Isoelectric Point: 4.6127

>AT86119 WP_001259253.1 NZ_CP023826:c2275418-2275167 [Escherichia coli]
MRTISYSEARQNLSATMMKAVEDHAPILITRQNGEACVLMSLEEYNSLEETAYLLRSPANARRLMDSIDSLKSDKGTEKD
IIE

Nucleotide

• Length: 252 bp

>AT86119 NZ_CP023826:c2275418-2275167 [Escherichia coli]
ATGCGTACAATTAGCTACAGCGAAGCGCGTCAGAATTTGTCGGCAACAATGATGAAAGCCGTTGAAGATCATGCCCCGAT
CCTCATTACTCGTCAGAATGGAGAGGCTTGTGTTCTGATGTCACTCGAAGAATACAACTCGCTGGAAGAGACGGCTTATC
TACTGCGTTCCCCCGCTAACGCCCGGAGATTGATGGACTCAATCGATAGCCTGAAATCAGACAAAGGAACGGAAAAGGAC
ATTATTGAGTGA

Structures

Toxin

• AlphaFold DB: E2QNP2

Antitoxin

• AlphaFold DB: E2QNP3