Detailed information of TA system TA086096

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 41722..42148
• Replicon: plasmid p7_2.1
• Accession: NZ_CP023821
• Organism: Escherichia coli strain 7/2

Toxin (Protein)

• Gene name: hok
• Uniprot ID: P11895
• Locus tag: CRT46_RS25365
• Protein ID: WP_001312861.1
• Coordinates: 41722..41880 (-)
• Length: 53 a.a.

Antitoxin (RNA)

Gene name	sok
Locus tag	-
Coordinates	41924..42148 (-)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CRT46_RS25315	36760..36987	-	228	WP_001254388.1	conjugal transfer relaxosome protein TraY	-
CRT46_RS25320	37081..37767	-	687	WP_000332487.1	PAS domain-containing protein	-
CRT46_RS25325	37961..38344	-	384	WP_001151564.1	relaxosome protein TraM	-
CRT46_RS25330	38630..39277	+	648	WP_124760006.1	transglycosylase SLT domain-containing protein	-
CRT46_RS25335	39574..40395	-	822	WP_001234469.1	DUF945 domain-containing protein	-
CRT46_RS25340	40516..40803	-	288	WP_000107537.1	hypothetical protein	-
CRT46_RS25365	41722..41880	-	159	WP_001312861.1	type I toxin-antitoxin system Hok family toxin	Toxin
-	41924..42148	-	225	NuclAT_0	-	Antitoxin
-	41924..42148	-	225	NuclAT_0	-	Antitoxin
-	41924..42148	-	225	NuclAT_0	-	Antitoxin
-	41924..42148	-	225	NuclAT_0	-	Antitoxin
CRT46_RS26650	41960..42148	+	189	WP_001299721.1	hypothetical protein	-
CRT46_RS25370	42160..42879	-	720	WP_001276234.1	plasmid SOS inhibition protein A	-
CRT46_RS25375	42876..43310	-	435	WP_000845901.1	conjugation system SOS inhibitor PsiB	-
CRT46_RS25380	43365..45323	-	1959	WP_032152921.1	ParB/RepB/Spo0J family partition protein	-
CRT46_RS25385	45382..45615	-	234	WP_000006018.1	DUF905 domain-containing protein	-
CRT46_RS25390	45671..46198	-	528	WP_000290793.1	single-stranded DNA-binding protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	aadA5 / qacE / sul1 / mph(A)	senB	1..113737	113737

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (8-48)

Sequences

Toxin

Protein

• Length: 53 a.a.
• Molecular weight: 6082.32 Da
• Isoelectric Point: 9.2584

>T86096 WP_001312861.1 NZ_CP023821:c41880-41722 [Escherichia coli]
MKLPRSSLVWCVLIVCLTLLIFTYLTRKSLCEIRYRDGHREVAAFMAYESGK

Nucleotide

• Length: 159 bp

>T86096 NZ_CP023821:c41880-41722 [Escherichia coli]
ATGAAACTACCACGAAGTTCCCTTGTCTGGTGTGTGTTGATCGTGTGTCTCACACTGTTGATATTCACTTATCTGACACG
AAAATCGCTGTGCGAGATTCGTTACAGAGACGGACACAGGGAGGTGGCGGCTTTCATGGCTTACGAATCCGGTAAGTAG

Antitoxin

Nucleotide

• Length: 225 bp

>AT86096 NZ_CP023821:c42148-41924 [Escherichia coli]
TCACACGGATTTCCCGTGAACGGTCTGAATGAGCGGATTATTTTCAGGGAAAGTGAGTGTGGTCAGCGTGCAGGTATATG
GGCTATGATGTGCCCGGCGCTTGAGGCTTTCTGCCTCATGACGTGAAGGTGGTTTGTTGCCGTGTTGTGTGGCAGAAAGA
AGATAGCCCCGTAGTAAGTTAATTTTCATTAACCACCACGAGGCATCCCTATGTCTAGTCCACAT

Structures

Toxin

• AlphaFold DB: P11895

Antitoxin