Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) yefM-yoeB (relBE)/YoeB-YefM
Location 2322683..2323185 Replicon chromosome
Accession NZ_CP023820
Organism Escherichia coli strain 7/2

Toxin (Protein)


Gene name yoeB Uniprot ID U9YDH0
Locus tag CRT46_RS11540 Protein ID WP_000767829.1
Coordinates 2322683..2322937 (-) Length 85 a.a.

Antitoxin (Protein)


Gene name yefM Uniprot ID S1Q063
Locus tag CRT46_RS11545 Protein ID WP_001259255.1
Coordinates 2322934..2323185 (-) Length 84 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
CRT46_RS11510 2317698..2317925 - 228 WP_000985273.1 sulfurtransferase TusA family protein -
CRT46_RS11515 2317939..2318997 - 1059 WP_000492324.1 transport protein YeeE -
CRT46_RS11520 2319176..2320534 - 1359 WP_000019197.1 putrescine/proton symporter PlaP -
CRT46_RS11525 2320524..2320586 - 63 WP_010723108.1 membrane protein YoeI -
CRT46_RS11530 2320801..2321730 - 930 WP_023154044.1 LysR family transcriptional regulator -
CRT46_RS11535 2321776..2322600 - 825 WP_000754758.1 SDR family oxidoreductase -
CRT46_RS11540 2322683..2322937 - 255 WP_000767829.1 type II toxin-antitoxin system mRNA interferase toxin YoeB Toxin
CRT46_RS11545 2322934..2323185 - 252 WP_001259255.1 YoeB-YefM toxin-antitoxin system antitoxin YefM Antitoxin
CRT46_RS11550 2323468..2323518 + 51 WP_001364200.1 his operon leader peptide -
CRT46_RS11555 2323664..2324563 + 900 WP_021523143.1 ATP phosphoribosyltransferase -
CRT46_RS11560 2324569..2325873 + 1305 WP_021523144.1 histidinol dehydrogenase -
CRT46_RS11565 2325870..2326940 + 1071 WP_000108937.1 histidinol-phosphate transaminase -
CRT46_RS11570 2326940..2328007 + 1068 WP_000080136.1 bifunctional histidinol-phosphatase/imidazoleglycerol-phosphate dehydratase HisB -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin YoeB

(2-84)

Antitoxin

Type II toxin-antitoxin system, antitoxin Phd/YefM

(1-73)


Sequences

Toxin        


Download         Length: 85 a.a.        Molecular weight: 10183.58 Da        Isoelectric Point: 8.0353

>T86084 WP_000767829.1 NZ_CP023820:c2322937-2322683 [Escherichia coli]
VKLIWSEESWDDYLYWQETDKRIVKKINELIKDTRRTPFEGKGKPEPLKHNLSGFWSRRITEEHRLVYAVTDDSLLIAAC
RYHY

Download         Length: 255 bp

>T86084 NZ_CP023820:c2322937-2322683 [Escherichia coli]
GTGAAACTAATCTGGTCTGAGGAATCATGGGATGATTATCTGTACTGGCAGGAAACAGATAAGCGAATTGTTAAAAAGAT
CAATGAACTTATCAAAGATACCCGCAGAACGCCATTTGAAGGTAAGGGGAAGCCAGAACCCCTGAAACATAATTTGTCAG
GTTTCTGGTCCCGACGCATTACAGAGGAGCACCGTCTGGTATACGCGGTTACCGACGATTCACTGCTCATTGCAGCATGT
CGTTATCATTATTGA

Antitoxin


Download         Length: 84 a.a.        Molecular weight: 9307.58 Da        Isoelectric Point: 4.7954

>AT86084 WP_001259255.1 NZ_CP023820:c2323185-2322934 [Escherichia coli]
MRTISYSEARQNLSATMMKAVEDHAPILITRQNGEACVLMSLEEYNSLEETAYLLRSPANARRLMDSIDSLKSGKGTEKD
IIE

Download         Length: 252 bp

>AT86084 NZ_CP023820:c2323185-2322934 [Escherichia coli]
ATGCGTACAATTAGCTACAGCGAAGCGCGTCAGAATTTGTCGGCAACAATGATGAAAGCCGTTGAAGATCATGCCCCGAT
CCTTATTACTCGTCAGAATGGAGAGGCTTGTGTTCTGATGTCACTCGAAGAATACAACTCGCTGGAAGAGACGGCTTATC
TACTGCGCTCCCCCGCTAACGCCCGGAGATTGATGGACTCAATCGATAGCCTGAAATCAGGCAAAGGAACAGAAAAGGAC
ATTATTGAGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 6NY6
PDB 2A6S
PDB 6OXA
PDB 2A6R
PDB 4V8X
PDB 6OTR
PDB 6OXI
PDB 2A6Q

Antitoxin

Source ID Structure
PDB 2A6Q
AlphaFold DB A0A7U9LNR0

References