Detailed information of TA system TA086069

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): ralRA/-
• Location: 1543988..1544359
• Replicon: chromosome
• Accession: NZ_CP023820
• Organism: Escherichia coli strain 7/2

Toxin (Protein)

• Gene name: ralR
• Uniprot ID: A0A0D6ZRD3
• Locus tag: CRT46_RS07480
• Protein ID: WP_001443846.1
• Coordinates: 1544210..1544359 (-)
• Length: 50 a.a.

Antitoxin (RNA)

Gene name	ralA
Locus tag	-
Coordinates	1543988..1544166 (+)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CRT46_RS07455	1539943..1541316	+	1374	WP_000123748.1	ATP-dependent RNA helicase DbpA	-
CRT46_RS07460	1541445..1542380	-	936	WP_001523172.1	tRNA 2-thiocytidine(32) synthetase TtcA	-
CRT46_RS07465	1542432..1543667	-	1236	WP_000040839.1	site-specific integrase	-
CRT46_RS07470	1543669..1543884	-	216	WP_000079604.1	excisionase XisR	-
CRT46_RS07475	1543984..1544172	-	189	WP_001302840.1	DUF1187 family protein	-
-	1543988..1544166	+	179	NuclAT_0	-	Antitoxin
-	1543988..1544166	+	179	NuclAT_0	-	Antitoxin
-	1543988..1544166	+	179	NuclAT_0	-	Antitoxin
-	1543988..1544166	+	179	NuclAT_0	-	Antitoxin
CRT46_RS07480	1544210..1544359	-	150	WP_001443846.1	type I toxin-antitoxin system endodeoxyribonuclease toxin RalR	Toxin
CRT46_RS07485	1544415..1545224	-	810	WP_000166313.1	recombination protein RecT	-
CRT46_RS07490	1545217..1547817	-	2601	WP_000105140.1	exodeoxyribonuclease VIII	-
CRT46_RS07495	1547919..1548194	-	276	WP_001344816.1	hypothetical protein	-
CRT46_RS07500	1548269..1548439	-	171	WP_001352098.1	conserved protein, Rac prophage	-
CRT46_RS07505	1548439..1548660	-	222	WP_000560223.1	killing protein KilR	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	-	1537619..1599286	61667

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Endodeoxyribonuclease toxin RalR (6-48)

Sequences

Toxin

Protein

• Length: 50 a.a.
• Molecular weight: 5383.12 Da
• Isoelectric Point: 8.3398

>T86069 WP_001443846.1 NZ_CP023820:c1544359-1544210 [Escherichia coli]
MRYDNVKPCPFCGCPSVTVKAISGYYRAKCNGCESRTGYGGSEKEALER

Nucleotide

• Length: 150 bp

>T86069 NZ_CP023820:c1544359-1544210 [Escherichia coli]
ATGAGATATGACAATGTTAAACCATGTCCATTTTGTGGTTGTCCATCAGTAACGGTGAAAGCCATTTCAGGATATTACCG
AGCGAAGTGTAACGGATGCGAATCCCGAACCGGTTATGGTGGAAGTGAAAAAGAAGCACTCGAACGATGA

Antitoxin

Nucleotide

• Length: 179 bp

>AT86069 NZ_CP023820:1543988-1544166 [Escherichia coli]
GAGGACTGAAGTTTCTCGCAATTAAAATTTATCAGTTTTACTTTCTGCTCTCTGGAAACGCCTGCTTCTTTTTTACCTGA
GAGCATTTTTTCGCATTCTGATTTCGTTAGTTTAGATTTTGAATATCTTGTCCAGTTAGTAGGAGTGCCACCTTCCTTTT
CAATAGTGGCGGTAATTTT

Structures

Toxin

• AlphaFold DB: A0A0D6ZRD3

Antitoxin