Detailed information of TA system TA086062

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): yafN-yafO (relBE)/YafO-YafN
• Location: 284541..285235
• Replicon: chromosome
• Accession: NZ_CP023820
• Organism: Escherichia coli strain 7/2

Toxin (Protein)

• Gene name: yafO
• Uniprot ID: A0A0D8WHS4
• Locus tag: CRT46_RS01285
• Protein ID: WP_001521903.1
• Coordinates: 284837..285235 (+)
• Length: 133 a.a.

Antitoxin (Protein)

• Gene name: yafN
• Uniprot ID: S1QAE3
• Locus tag: CRT46_RS01280
• Protein ID: WP_000554758.1
• Coordinates: 284541..284834 (+)
• Length: 98 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CRT46_RS01255	279656..280405	+	750	WP_016233951.1	C40 family peptidase	-
CRT46_RS01260	280462..280815	-	354	WP_024166466.1	type II toxin-antitoxin system HicB family antitoxin	-
CRT46_RS01265	280894..282606	-	1713	Protein_246	flagellar biosynthesis protein FlhA	-
CRT46_RS01270	282578..283363	+	786	WP_000207556.1	putative lateral flagellar export/assembly protein LafU	-
CRT46_RS01275	283434..284489	+	1056	WP_001226177.1	DNA polymerase IV	-
CRT46_RS01280	284541..284834	+	294	WP_000554758.1	type I toxin-antitoxin system antitoxin YafN	Antitoxin
CRT46_RS01285	284837..285235	+	399	WP_001521903.1	type II toxin-antitoxin system mRNA interferase toxin YafO	Toxin
CRT46_RS01290	285245..285697	+	453	WP_023144376.1	GNAT family N-acetyltransferase	-
CRT46_RS01295	285942..286148	+	207	Protein_252	RtcB family protein	-
CRT46_RS01300	286144..286496	+	353	Protein_253	peptide chain release factor H	-
CRT46_RS01305	286553..288010	-	1458	WP_001293009.1	cytosol nonspecific dipeptidase	-
CRT46_RS01310	288271..288729	+	459	WP_001291990.1	xanthine phosphoribosyltransferase	-
CRT46_RS01315	288821..290065	+	1245	WP_000189541.1	esterase FrsA	-
-	289325..289405	+	81	NuclAT_11	-	-
-	289325..289405	+	81	NuclAT_11	-	-
-	289325..289405	+	81	NuclAT_11	-	-
-	289325..289405	+	81	NuclAT_11	-	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase toxin YafO (27-130)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 133 a.a.
• Molecular weight: 15471.88 Da
• Isoelectric Point: 8.0949

>T86062 WP_001521903.1 NZ_CP023820:284837-285235 [Escherichia coli]
MRVFKTKLIRLQLTAEELDALTADFISYKRDGILPDIFGRDALYDDSFTWPLIKFERVAHIHLANVNNPFPPQLRQFSRT
NDEAHLVYCQGAFDEQAWLLIAILKPEPHKLARDNNQMHKIGKMAEAFRMRF

Nucleotide

• Length: 399 bp

>T86062 NZ_CP023820:284837-285235 [Escherichia coli]
ATGCGGGTATTCAAAACAAAACTTATTCGCCTGCAACTTACAGCAGAGGAACTTGATGCGTTAACGGCGGATTTTATTTC
CTATAAGCGTGACGGTATTTTGCCAGATATATTTGGTCGCGATGCACTCTACGACGACTCGTTTACCTGGCCATTAATCA
AATTTGAGCGAGTTGCTCATATTCATCTGGCAAATGTGAATAATCCATTTCCGCCACAGTTGCGCCAATTCAGCAGAACG
AATGACGAAGCGCATTTGGTATATTGTCAGGGGGCGTTTGATGAGCAAGCATGGTTGCTCATTGCCATTCTGAAGCCTGA
ACCTCATAAACTGGCTCGAGATAACAACCAAATGCATAAAATTGGAAAAATGGCAGAAGCGTTTCGCATGCGTTTTTGA

Antitoxin

Protein

• Length: 98 a.a.
• Molecular weight: 11233.76 Da
• Isoelectric Point: 5.2170

>AT86062 WP_000554758.1 NZ_CP023820:284541-284834 [Escherichia coli]
MHRILAEKSVNITELRKNPAKYFIDQPVAVLSNNRPAGYLLSASAFEALMDMLAEQEEKKPIKARFRPSAARLEEITRRA
EQYLNDMTDDDFNDFKE

Nucleotide

• Length: 294 bp

>AT86062 NZ_CP023820:284541-284834 [Escherichia coli]
ATGCATCGAATTCTCGCTGAAAAATCGGTCAATATCACTGAGTTACGTAAAAACCCAGCTAAATACTTTATTGATCAACC
GGTTGCGGTTCTTTCTAATAATCGCCCCGCAGGATATCTCTTAAGTGCCAGCGCATTCGAAGCGTTAATGGACATGCTTG
CTGAACAAGAGGAGAAAAAGCCCATAAAGGCGCGCTTCCGTCCGAGTGCTGCAAGATTAGAGGAAATTACACGCCGCGCT
GAACAATATCTTAATGATATGACGGATGATGATTTCAATGACTTTAAGGAATAA

Structures

Toxin

• AlphaFold DB: A0A0D8WHS4

Antitoxin

• AlphaFold DB: S1QAE3