Detailed information of TA system TA086049

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 19821..20061
• Replicon: plasmid pEcIMT16316
• Accession: NZ_CP023816
• Organism: Escherichia coli strain IMT16316

Toxin (Protein)

• Gene name: hok
• Uniprot ID: P11895
• Locus tag: CRI72_RS25145
• Protein ID: WP_001312861.1
• Coordinates: 19821..19979 (-)
• Length: 53 a.a.

Antitoxin (RNA)

Gene name	sok
Locus tag	-
Coordinates	20023..20061 (-)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CRI72_RS25105	14933..15160	-	228	WP_001254388.1	conjugal transfer relaxosome protein TraY	-
CRI72_RS25110	15248..15925	-	678	WP_001348626.1	PAS domain-containing protein	-
CRI72_RS25115	16059..16442	-	384	WP_001151566.1	relaxosome protein TraM	-
CRI72_RS25120	16773..17375	+	603	WP_000243712.1	transglycosylase SLT domain-containing protein	-
CRI72_RS25125	17672..18493	-	822	WP_001234469.1	DUF945 domain-containing protein	-
CRI72_RS25130	18612..18899	-	288	WP_000107535.1	hypothetical protein	-
CRI72_RS26425	18924..19130	-	207	WP_000275859.1	hypothetical protein	-
CRI72_RS26430	19043..19378	-	336	WP_013023876.1	hypothetical protein	-
CRI72_RS25145	19821..19979	-	159	WP_001312861.1	type I toxin-antitoxin system Hok family toxin	Toxin
-	20023..20061	-	39	NuclAT_1	-	Antitoxin
-	20023..20061	-	39	NuclAT_1	-	Antitoxin
-	20023..20061	-	39	NuclAT_1	-	Antitoxin
-	20023..20061	-	39	NuclAT_1	-	Antitoxin
-	21501..21603	-	103	NuclAT_0	-	-
-	21501..21603	-	103	NuclAT_0	-	-
-	21501..21603	-	103	NuclAT_0	-	-
-	21501..21603	-	103	NuclAT_0	-	-
CRI72_RS25155	21615..22334	-	720	WP_001276232.1	plasmid SOS inhibition protein A	-
CRI72_RS25160	22331..22765	-	435	WP_000845940.1	conjugation system SOS inhibitor PsiB	-
CRI72_RS25165	22820..24778	-	1959	WP_000117179.1	ParB/RepB/Spo0J family partition protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	tet(B) / tet(A) / blaTEM-1B / mph(A) / sul1 / qacE / aadA5 / dfrA17 / blaCTX-M-15 / aph(6)-Id / aph(3'')-Ib / sul2 / aac(3)-IId	-	1..145883	145883

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (8-48)

Sequences

Toxin

Protein

• Length: 53 a.a.
• Molecular weight: 6082.32 Da
• Isoelectric Point: 9.2584

>T86049 WP_001312861.1 NZ_CP023816:c19979-19821 [Escherichia coli]
MKLPRSSLVWCVLIVCLTLLIFTYLTRKSLCEIRYRDGHREVAAFMAYESGK

Nucleotide

• Length: 159 bp

>T86049 NZ_CP023816:c19979-19821 [Escherichia coli]
ATGAAACTACCACGAAGTTCCCTTGTCTGGTGTGTGTTGATCGTGTGTCTCACACTGTTGATATTCACTTATCTGACACG
AAAATCGCTGTGCGAGATTCGTTACAGAGACGGACACAGAGAGGTGGCGGCTTTCATGGCTTACGAATCCGGTAAGTAG

Antitoxin

Nucleotide

• Length: 39 bp

>AT86049 NZ_CP023816:c20061-20023 [Escherichia coli]
CATTAACCACCACGAGGCATCCCTATGTCTAGTCCACAT

Structures

Toxin

• AlphaFold DB: P11895

Antitoxin