Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) yefM-yoeB (relBE)/YoeB-YefM
Location 3814093..3814595 Replicon chromosome
Accession NZ_CP023815
Organism Escherichia coli strain IMT16316

Toxin (Protein)


Gene name yoeB Uniprot ID U9YDH0
Locus tag CRI72_RS18995 Protein ID WP_000767829.1
Coordinates 3814341..3814595 (+) Length 85 a.a.

Antitoxin (Protein)


Gene name yefM Uniprot ID S1Q063
Locus tag CRI72_RS18990 Protein ID WP_001259255.1
Coordinates 3814093..3814344 (+) Length 84 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
CRI72_RS18960 3809270..3810337 - 1068 WP_000080101.1 bifunctional histidinol-phosphatase/imidazoleglycerol-phosphate dehydratase HisB -
CRI72_RS18965 3810337..3811407 - 1071 WP_001099214.1 histidinol-phosphate transaminase -
CRI72_RS18970 3811404..3812708 - 1305 WP_001396164.1 histidinol dehydrogenase -
CRI72_RS18975 3812714..3813613 - 900 WP_000566782.1 ATP phosphoribosyltransferase -
CRI72_RS18985 3813759..3813809 - 51 WP_001364200.1 his operon leader peptide -
CRI72_RS18990 3814093..3814344 + 252 WP_001259255.1 YoeB-YefM toxin-antitoxin system antitoxin YefM Antitoxin
CRI72_RS18995 3814341..3814595 + 255 WP_000767829.1 type II toxin-antitoxin system mRNA interferase toxin YoeB Toxin
CRI72_RS19000 3814678..3815502 + 825 WP_000754754.1 SDR family oxidoreductase -
CRI72_RS19005 3815548..3816477 + 930 WP_000803351.1 LysR family transcriptional regulator -
CRI72_RS19010 3816692..3816754 + 63 WP_010723108.1 membrane protein YoeI -
CRI72_RS19015 3816744..3818102 + 1359 WP_000019197.1 putrescine/proton symporter PlaP -
CRI72_RS19020 3818281..3819339 + 1059 WP_000492305.1 transport protein YeeE -
CRI72_RS19025 3819353..3819580 + 228 WP_000985273.1 sulfurtransferase TusA family protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin YoeB

(2-84)

Antitoxin

Type II toxin-antitoxin system, antitoxin Phd/YefM

(1-73)


Sequences

Toxin        


Download         Length: 85 a.a.        Molecular weight: 10183.58 Da        Isoelectric Point: 8.0353

>T86038 WP_000767829.1 NZ_CP023815:3814341-3814595 [Escherichia coli]
VKLIWSEESWDDYLYWQETDKRIVKKINELIKDTRRTPFEGKGKPEPLKHNLSGFWSRRITEEHRLVYAVTDDSLLIAAC
RYHY

Download         Length: 255 bp

>T86038 NZ_CP023815:3814341-3814595 [Escherichia coli]
GTGAAACTAATCTGGTCTGAGGAATCATGGGATGATTATCTGTACTGGCAGGAAACAGATAAGCGAATTGTTAAAAAGAT
CAATGAACTTATCAAAGATACCCGCAGAACGCCATTTGAAGGTAAGGGGAAGCCAGAACCCCTGAAACATAATTTGTCAG
GTTTCTGGTCCCGACGCATTACAGAGGAGCACCGTCTGGTATACGCGGTTACCGACGATTCACTGCTCATTGCAGCATGT
CGTTATCATTATTGA

Antitoxin


Download         Length: 84 a.a.        Molecular weight: 9307.58 Da        Isoelectric Point: 4.7954

>AT86038 WP_001259255.1 NZ_CP023815:3814093-3814344 [Escherichia coli]
MRTISYSEARQNLSATMMKAVEDHAPILITRQNGEACVLMSLEEYNSLEETAYLLRSPANARRLMDSIDSLKSGKGTEKD
IIE

Download         Length: 252 bp

>AT86038 NZ_CP023815:3814093-3814344 [Escherichia coli]
ATGCGTACAATTAGCTACAGCGAAGCGCGTCAGAATTTGTCGGCAACAATGATGAAAGCCGTTGAAGATCATGCCCCGAT
CCTTATTACTCGTCAGAATGGAGAGGCTTGTGTTCTGATGTCACTCGAAGAATACAACTCGCTGGAAGAGACGGCTTATC
TACTGCGTTCCCCCGCTAACGCCCGGAGATTGATGGACTCAATCGATAGCCTGAAATCAGGCAAAGGAACAGAAAAGGAC
ATTATTGAGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 6NY6
PDB 2A6S
PDB 6OXA
PDB 2A6R
PDB 4V8X
PDB 6OTR
PDB 6OXI
PDB 2A6Q

Antitoxin

Source ID Structure
PDB 2A6Q
AlphaFold DB A0A7U9LNR0

References