Detailed information of TA system TA086033

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HTH(antitoxin)
• Location: 1672272..1672874
• Replicon: chromosome
• Accession: NZ_CP023815
• Organism: Escherichia coli strain IMT16316

Toxin (Protein)

• Gene name: higB
• Uniprot ID: U9XIS6
• Locus tag: CRI72_RS08370
• Protein ID: WP_000897305.1
• Coordinates: 1672563..1672874 (-)
• Length: 104 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: CRI72_RS08365
• Protein ID: WP_000356397.1
• Coordinates: 1672272..1672562 (-)
• Length: 97 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CRI72_RS08340	1668774..1669676	+	903	WP_000331377.1	formate dehydrogenase O subunit beta	-
CRI72_RS08345	1669673..1670308	+	636	WP_000829013.1	formate dehydrogenase cytochrome b556 subunit	-
CRI72_RS08350	1670305..1671234	+	930	WP_000027720.1	formate dehydrogenase accessory protein FdhE	-
CRI72_RS08355	1671449..1671667	-	219	WP_001298592.1	ribbon-helix-helix domain-containing protein	-
CRI72_RS08365	1672272..1672562	-	291	WP_000356397.1	helix-turn-helix domain-containing protein	Antitoxin
CRI72_RS08370	1672563..1672874	-	312	WP_000897305.1	hypothetical protein	Toxin
CRI72_RS08375	1673103..1674011	+	909	WP_001318161.1	alpha/beta hydrolase	-
CRI72_RS08380	1674075..1675016	-	942	WP_001297068.1	fatty acid biosynthesis protein FabY	-
CRI72_RS08385	1675061..1675498	-	438	WP_000560983.1	D-tyrosyl-tRNA(Tyr) deacylase	-
CRI72_RS08390	1675495..1676367	-	873	WP_000920762.1	virulence factor BrkB family protein	-
CRI72_RS08395	1676361..1676960	-	600	WP_001314324.1	glucose-1-phosphatase	-
CRI72_RS08400	1677059..1677844	-	786	WP_000059679.1	DeoR/GlpR family DNA-binding transcription regulator	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

No domain identified.

Antitoxin

• Cro/C1-type helix-turn-helix domain (36-85)

Sequences

Toxin

Protein

• Length: 104 a.a.
• Molecular weight: 12190.19 Da
• Isoelectric Point: 9.7791

>T86033 WP_000897305.1 NZ_CP023815:c1672874-1672563 [Escherichia coli]
MLFIETEIFTEDVQKLLTDDEFSRFQFFLALNPDYGEVIPETGGLRKVRWVSGGKGKRAGVRVIYFHQVKHYEIRLLLIY
RKGIKDDLSPQEKAMLRLLNTRW

Nucleotide

• Length: 312 bp

>T86033 NZ_CP023815:c1672874-1672563 [Escherichia coli]
ATGCTCTTCATCGAAACGGAAATTTTTACTGAAGATGTTCAAAAACTGCTGACCGACGATGAATTCAGTCGTTTTCAGTT
TTTCCTGGCCTTGAATCCTGACTATGGTGAGGTAATACCTGAAACTGGTGGGTTAAGAAAGGTTCGATGGGTGTCAGGAG
GAAAGGGGAAACGTGCTGGTGTCAGGGTTATTTACTTTCATCAGGTTAAACATTATGAGATCAGATTACTGCTTATTTAT
CGTAAGGGTATTAAAGATGATCTGTCTCCCCAGGAAAAAGCAATGCTCCGGTTGTTAAATACGAGGTGGTAA

Antitoxin

Protein

• Length: 97 a.a.
• Molecular weight: 10846.43 Da
• Isoelectric Point: 10.8907

>AT86033 WP_000356397.1 NZ_CP023815:c1672562-1672272 [Escherichia coli]
MDKALFERLTHSMAQMNEIIEGTRQPSRTFEVDAMKIKEIRRASGLSQSKFADLISVSVDTLRNWEQGRRSPTGPAKALL
RAIANDPQHVLQALNR

Nucleotide

• Length: 291 bp

>AT86033 NZ_CP023815:c1672562-1672272 [Escherichia coli]
ATGGATAAGGCACTTTTTGAGCGGTTGACTCACAGCATGGCCCAGATGAATGAAATTATTGAAGGAACGCGACAGCCTTC
GCGGACTTTTGAAGTTGATGCTATGAAAATCAAAGAAATACGCCGCGCTTCAGGGTTGTCACAATCTAAATTTGCCGATC
TTATTTCTGTAAGCGTAGACACGTTGCGTAACTGGGAGCAGGGGCGACGTTCACCGACCGGGCCTGCAAAGGCTTTGCTG
CGAGCGATTGCCAATGATCCGCAACATGTGCTGCAAGCACTTAACCGTTAG

Structures

Toxin

• AlphaFold DB: A0A0E0Y7I7