Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) yafN-yafO (relBE)/YafO-YafN
Location 755345..756039 Replicon chromosome
Accession NZ_CP023815
Organism Escherichia coli strain IMT16316

Toxin (Protein)


Gene name yafO Uniprot ID Q0T7Q5
Locus tag CRI72_RS03800 Protein ID WP_001263491.1
Coordinates 755345..755743 (-) Length 133 a.a.

Antitoxin (Protein)


Gene name yafN Uniprot ID L4JHF0
Locus tag CRI72_RS03805 Protein ID WP_000554755.1
Coordinates 755746..756039 (-) Length 98 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
CRI72_RS03765 751296..751577 + 282 WP_022645226.1 hypothetical protein -
CRI72_RS03770 751594..752103 - 510 WP_001361775.1 hydrolase -
CRI72_RS03775 752165..752779 - 615 WP_022645225.1 peptide chain release factor H -
CRI72_RS03780 752776..753915 - 1140 WP_022645224.1 RNA ligase RtcB family protein -
CRI72_RS03785 754106..754327 - 222 Protein_692 GNAT family N-acetyltransferase -
CRI72_RS03790 754376..755073 + 698 Protein_693 IS1 family transposase -
CRI72_RS03795 755090..755335 - 246 Protein_694 GNAT family N-acetyltransferase -
CRI72_RS03800 755345..755743 - 399 WP_001263491.1 type II toxin-antitoxin system mRNA interferase toxin YafO Toxin
CRI72_RS03805 755746..756039 - 294 WP_000554755.1 type I toxin-antitoxin system antitoxin YafN Antitoxin
CRI72_RS03810 756091..757146 - 1056 WP_022645222.1 DNA polymerase IV -
CRI72_RS03815 757217..758002 - 786 WP_072224360.1 putative lateral flagellar export/assembly protein LafU -
CRI72_RS03820 757974..759686 + 1713 Protein_699 flagellar biosynthesis protein FlhA -
CRI72_RS03825 759784..760557 - 774 WP_022645219.1 C40 family peptidase -
CRI72_RS03830 760743..761003 + 261 WP_000729708.1 type II toxin-antitoxin system antitoxin DinJ -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase toxin YafO

(27-130)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 133 a.a.        Molecular weight: 15457.86 Da        Isoelectric Point: 8.0949

>T86027 WP_001263491.1 NZ_CP023815:c755743-755345 [Escherichia coli]
MRVFKTKLIRLQLTAEELDALTADFISYKRDGVLPDIFGRDALYDDSFTWPLIKFERVAHIHLANVNNPFPPQLRQFSRT
NDEAHLVYCQGAFDEQAWLLIAILKPEPHKLARDNNQMHKIGKMAEAFRMRF

Download         Length: 399 bp

>T86027 NZ_CP023815:c755743-755345 [Escherichia coli]
ATGCGGGTATTCAAAACAAAACTTATTCGCCTGCAACTTACAGCAGAGGAACTTGATGCGTTAACAGCGGATTTTATTTC
CTATAAGCGTGACGGTGTTTTGCCAGATATATTTGGTCGCGATGCACTCTACGACGACTCGTTTACCTGGCCATTAATCA
AATTTGAGCGAGTTGCTCATATTCATCTGGCAAATGTGAATAATCCATTTCCGCCACAGTTGCGCCAATTCAGCAGAACG
AATGACGAAGCGCATTTGGTATATTGTCAGGGTGCGTTTGATGAGCAAGCATGGTTGCTCATTGCCATTCTGAAACCTGA
ACCTCATAAACTGGCTCGAGATAACAACCAAATGCATAAAATTGGGAAAATGGCAGAAGCGTTTCGCATGCGTTTTTGA

Antitoxin


Download         Length: 98 a.a.        Molecular weight: 11235.78 Da        Isoelectric Point: 5.2170

>AT86027 WP_000554755.1 NZ_CP023815:c756039-755746 [Escherichia coli]
MHRILAEKSVNITELRKNPAKYFIDQPVAVLSNNRPAGYLLSASAFEALMDMLAEQEEKKLVKARFRPSAARLEEITRRA
EQYLNDMTDDDFNDFKE

Download         Length: 294 bp

>AT86027 NZ_CP023815:c756039-755746 [Escherichia coli]
ATGCATCGAATTCTCGCTGAAAAATCGGTCAATATCACTGAGTTACGTAAAAACCCAGCTAAATACTTTATTGATCAACC
GGTTGCGGTTCTTTCTAATAATCGCCCCGCAGGATATCTCTTAAGTGCCAGCGCATTTGAAGCGTTAATGGACATGCTTG
CTGAACAAGAGGAGAAAAAACTGGTTAAGGCGCGCTTCCGTCCGAGTGCCGCAAGATTAGAGGAAATTACACGCCGCGCT
GAACAATATCTTAATGATATGACGGATGATGATTTCAATGACTTTAAGGAATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A4P7TPK7

Antitoxin

Source ID Structure
AlphaFold DB A0A829G9H5

References