Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/Tad-HTH_37
Location 4020748..4021352 Replicon chromosome
Accession NZ_CP023737
Organism Methylosinus trichosporium OB3b

Toxin (Protein)


Gene name higB Uniprot ID A0A1A6FMI3
Locus tag CQW49_RS19020 Protein ID WP_003612979.1
Coordinates 4020748..4021065 (+) Length 106 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID A0A1A6FMG2
Locus tag CQW49_RS19025 Protein ID WP_003612976.1
Coordinates 4021062..4021352 (+) Length 97 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
CQW49_RS19005 4016373..4018178 - 1806 WP_099831827.1 tetratricopeptide repeat protein -
CQW49_RS19010 4018325..4019524 - 1200 WP_003612982.1 ADP-forming succinate--CoA ligase subunit beta -
CQW49_RS19015 4019601..4020563 - 963 WP_003612980.1 malate dehydrogenase -
CQW49_RS19020 4020748..4021065 + 318 WP_003612979.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
CQW49_RS19025 4021062..4021352 + 291 WP_003612976.1 helix-turn-helix transcriptional regulator Antitoxin
CQW49_RS19030 4021383..4021793 - 411 WP_003612974.1 EVE domain-containing protein -
CQW49_RS19035 4021793..4022080 - 288 WP_003612973.1 YciI family protein -
CQW49_RS19040 4022236..4022736 + 501 WP_003612972.1 DUF2165 domain-containing protein -
CQW49_RS19045 4022852..4024111 + 1260 WP_003612971.1 c-type cytochrome -
CQW49_RS19050 4024320..4024835 + 516 WP_024749488.1 YchJ family protein -
CQW49_RS19055 4024959..4025453 + 495 WP_003612966.1 peptidoglycan-binding protein LysM -
CQW49_RS19060 4025743..4026030 + 288 WP_024749487.1 co-chaperone GroES -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin HigB-like

(15-102)

Antitoxin

Cro/C1-type helix-turn-helix domain

(40-86)


Sequences

Toxin        


Download         Length: 106 a.a.        Molecular weight: 12151.32 Da        Isoelectric Point: 10.9010

>T85983 WP_003612979.1 NZ_CP023737:4020748-4021065 [Methylosinus trichosporium OB3b]
MWSVEYLPAAAEEEAALPIEMQARLARMLDVIRRAGLTNLPRDWVKPLEGKLWELRITGKDGVARAIYVTAEGRRVVIVR
IFVKKTQKTPRRELELARRRAKEVE

Download         Length: 318 bp

>T85983 NZ_CP023737:4020748-4021065 [Methylosinus trichosporium OB3b]
ATGTGGTCTGTCGAATATCTGCCCGCTGCGGCCGAAGAGGAAGCGGCGCTTCCGATCGAGATGCAGGCGAGACTCGCCCG
GATGCTCGATGTCATCCGCCGTGCGGGGCTGACAAATTTGCCGCGCGACTGGGTGAAGCCGCTCGAGGGCAAATTGTGGG
AACTGCGGATCACCGGGAAGGACGGCGTCGCCCGCGCCATCTATGTCACAGCTGAAGGACGGCGCGTGGTCATCGTTCGA
ATCTTCGTGAAGAAGACGCAAAAAACGCCGCGGCGCGAGCTGGAGCTCGCTCGACGACGGGCGAAGGAGGTCGAATGA

Antitoxin


Download         Length: 97 a.a.        Molecular weight: 10787.22 Da        Isoelectric Point: 9.5881

>AT85983 WP_003612976.1 NZ_CP023737:4021062-4021352 [Methylosinus trichosporium OB3b]
MTKTIDARIIHAKRMAEDEAYRAAYDALEEEFALVNSMIEARARSKLSQAEVAKRMGTTESAVSRLESGRGKPSTRTLER
YAEAVGHRLRITFEPS

Download         Length: 291 bp

>AT85983 NZ_CP023737:4021062-4021352 [Methylosinus trichosporium OB3b]
ATGACCAAGACGATCGACGCGCGAATCATTCATGCGAAGCGGATGGCCGAAGACGAGGCCTATCGCGCCGCCTATGACGC
GCTCGAAGAGGAGTTCGCTCTCGTCAATTCCATGATCGAGGCGCGAGCGCGTTCCAAATTGTCGCAGGCGGAAGTGGCGA
AGCGCATGGGCACGACGGAAAGCGCGGTGTCGAGGCTCGAATCCGGGCGCGGCAAACCATCGACGCGCACATTGGAACGC
TACGCCGAAGCCGTCGGGCATCGGCTGCGGATCACATTCGAGCCCTCGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A1A6FMI3

Antitoxin

Source ID Structure
AlphaFold DB A0A1A6FMG2

References