Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/HigB-VapI
Location 3214998..3215730 Replicon chromosome
Accession NZ_CP023737
Organism Methylosinus trichosporium OB3b

Toxin (Protein)


Gene name higB Uniprot ID A0A1A6FRG6
Locus tag CQW49_RS15450 Protein ID WP_003615036.1
Coordinates 3215452..3215730 (-) Length 93 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID A0A1A6FTV8
Locus tag CQW49_RS15445 Protein ID WP_003615038.1
Coordinates 3214998..3215324 (-) Length 109 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
CQW49_RS15415 3210323..3210679 - 357 WP_003615049.1 hypothetical protein -
CQW49_RS15420 3210749..3211399 - 651 WP_003615048.1 Fic family protein -
CQW49_RS24675 3211447..3211659 - 213 WP_155931257.1 hypothetical protein -
CQW49_RS15425 3211773..3212513 - 741 WP_003615045.1 sulfite exporter TauE/SafE family protein -
CQW49_RS15430 3212628..3213527 - 900 WP_003615043.1 dihydrodipicolinate synthase family protein -
CQW49_RS15435 3213536..3213988 - 453 WP_003615041.1 cupin domain-containing protein -
CQW49_RS15440 3214099..3214989 + 891 WP_003615039.1 LysR family transcriptional regulator -
CQW49_RS15445 3214998..3215324 - 327 WP_003615038.1 HigA family addiction module antidote protein Antitoxin
CQW49_RS15450 3215452..3215730 - 279 WP_003615036.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
CQW49_RS15455 3215847..3216749 + 903 WP_003615034.1 tryptophan synthase subunit alpha -
CQW49_RS15460 3216746..3217606 + 861 WP_003615033.1 acetyl-CoA carboxylase, carboxyltransferase subunit beta -
CQW49_RS15465 3217626..3218930 + 1305 WP_003615029.1 bifunctional folylpolyglutamate synthase/dihydrofolate synthase -
CQW49_RS15470 3218980..3220155 - 1176 WP_024749364.1 IS4 family transposase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin HigB-1

(12-92)

Antitoxin

Toxin-antitoxin system, antidote protein, HigA

(20-95)


Sequences

Toxin        


Download         Length: 93 a.a.        Molecular weight: 10329.69 Da        Isoelectric Point: 7.2044

>T85982 WP_003615036.1 NZ_CP023737:c3215730-3215452 [Methylosinus trichosporium OB3b]
MIRSVKGTVTRQFVETGKSKFSGLDEALARQRLAELDSAASLDDLPPLRSVGLHRLSGDRKGQWAIKVNGPWRIAFRFED
GDAFEVEIVDYH

Download         Length: 279 bp

>T85982 NZ_CP023737:c3215730-3215452 [Methylosinus trichosporium OB3b]
ATGATACGCTCCGTCAAAGGCACGGTGACTCGGCAGTTCGTCGAGACCGGCAAGTCGAAGTTTTCCGGCCTCGACGAAGC
GCTCGCGCGGCAACGTCTGGCGGAGCTCGACAGCGCCGCCTCGCTCGATGACCTTCCACCGCTTCGTAGCGTCGGACTTC
ACCGGCTCTCGGGTGACCGCAAGGGCCAATGGGCGATCAAAGTGAATGGCCCCTGGCGGATCGCCTTTCGATTCGAGGAC
GGCGATGCGTTCGAGGTGGAGATCGTCGACTATCATTGA

Antitoxin


Download         Length: 109 a.a.        Molecular weight: 11696.42 Da        Isoelectric Point: 10.2107

>AT85982 WP_003615038.1 NZ_CP023737:c3215324-3214998 [Methylosinus trichosporium OB3b]
MTTKSADIFEAPPGGFRFGPIHPGRTLAAELKARGISAHALALSLRVPANRISEIVAGKRGVTAETALRLARYLGTSAAF
WMNLQSKYDLEIAERDFGERIRAEVEAA

Download         Length: 327 bp

>AT85982 NZ_CP023737:c3215324-3214998 [Methylosinus trichosporium OB3b]
ATGACGACGAAAAGCGCGGACATCTTCGAGGCCCCGCCCGGCGGCTTCCGTTTCGGCCCGATCCATCCGGGCCGCACCCT
CGCCGCCGAGCTGAAAGCGCGCGGAATCAGCGCTCACGCGCTGGCGCTGAGCTTGCGGGTGCCCGCCAACCGGATCAGCG
AGATCGTCGCTGGAAAGCGCGGCGTCACCGCGGAGACCGCGCTGCGCCTCGCCCGCTATCTCGGCACGAGCGCGGCCTTC
TGGATGAATCTGCAGTCGAAATATGATCTCGAGATCGCCGAGCGCGATTTCGGCGAGCGGATCAGGGCGGAAGTCGAAGC
GGCCTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A1A6FRG6

Antitoxin

Source ID Structure
AlphaFold DB A0A1A6FTV8

References