Detailed information of TA system TA085979

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/Tad-HTH_31
• Location: 1655441..1656042
• Replicon: chromosome
• Accession: NZ_CP023737
• Organism: Methylosinus trichosporium OB3b

Toxin (Protein)

• Gene name: higB
• Uniprot ID: -
• Locus tag: CQW49_RS08025
• Protein ID: WP_003615898.1
• Coordinates: 1655441..1655764 (+)
• Length: 108 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: A0A1A6FQK6
• Locus tag: CQW49_RS08030
• Protein ID: WP_003615896.1
• Coordinates: 1655761..1656042 (+)
• Length: 94 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CQW49_RS08020	1652260..1655109	-	2850	WP_003615900.1	DEAD/DEAH box helicase	-
CQW49_RS08025	1655441..1655764	+	324	WP_003615898.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
CQW49_RS08030	1655761..1656042	+	282	WP_003615896.1	helix-turn-helix transcriptional regulator	Antitoxin
CQW49_RS08035	1656377..1657105	-	729	WP_024749851.1	IS21-like element helper ATPase IstB	-
CQW49_RS08040	1657105..1657389	-	285	Protein_1576	IS21 family transposase	-
CQW49_RS08045	1657420..1657653	-	234	WP_003616132.1	hypothetical protein	-
CQW49_RS08050	1657656..1659266	-	1611	WP_003616133.1	IS66 family transposase	-
CQW49_RS08055	1659335..1659682	-	348	WP_024749862.1	IS66 family insertion sequence element accessory protein TnpB	-
CQW49_RS08060	1659679..1660041	-	363	WP_051418692.1	transposase	-
CQW49_RS08065	1660629..1661006	+	378	WP_003613712.1	IS66 family insertion sequence hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-like (15-104)

Antitoxin

• Cro/C1-type helix-turn-helix domain (35-83)

Sequences

Toxin

Protein

• Length: 108 a.a.
• Molecular weight: 12084.25 Da
• Isoelectric Point: 10.8882

>T85979 WP_003615898.1 NZ_CP023737:1655441-1655764 [Methylosinus trichosporium OB3b]
MAWRVEILNETVVAEIAALPADMQARFLRLAERINSAGLESLSEPHVKHLEGKLWELRLTGRDGIARALYVTTVGRKVVV
LRAFVKKTQKTPRAEIELALRRAKEVI

Nucleotide

• Length: 324 bp

>T85979 NZ_CP023737:1655441-1655764 [Methylosinus trichosporium OB3b]
ATGGCTTGGCGCGTCGAAATCCTGAACGAGACAGTCGTCGCGGAAATCGCCGCCCTTCCGGCGGACATGCAGGCGCGGTT
TCTCCGGCTTGCCGAGCGCATAAACTCGGCCGGTCTCGAAAGTCTGAGCGAGCCACACGTAAAGCACCTAGAAGGGAAAC
TGTGGGAATTACGCCTAACGGGCCGAGACGGAATAGCCCGAGCGCTTTACGTCACGACGGTCGGCCGCAAGGTCGTGGTG
CTCAGGGCCTTTGTGAAGAAGACCCAAAAAACGCCACGCGCCGAAATCGAGCTTGCGTTACGTCGAGCAAAGGAGGTGAT
ATGA

Antitoxin

Protein

• Length: 94 a.a.
• Molecular weight: 10063.67 Da
• Isoelectric Point: 10.5567

>AT85979 WP_003615896.1 NZ_CP023737:1655761-1656042 [Methylosinus trichosporium OB3b]
MTIPFEKIKARLMTNPAVKAEYDALAPEFEISAELVKARLRAGLSQAELATRMGTSQSAIARLESGQTLPSTKTLLRFAE
ATGSKFHVRLSAA

Nucleotide

• Length: 282 bp

>AT85979 NZ_CP023737:1655761-1656042 [Methylosinus trichosporium OB3b]
ATGACGATTCCATTCGAAAAGATCAAAGCTCGCCTTATGACCAACCCCGCGGTCAAGGCCGAATACGACGCGCTCGCTCC
CGAATTCGAGATTTCCGCAGAGCTGGTCAAAGCTCGCCTTCGTGCCGGCCTCTCTCAGGCCGAATTGGCGACACGCATGG
GCACGAGCCAGTCGGCGATTGCTCGGCTGGAGAGCGGGCAAACGTTGCCGAGCACGAAGACTCTTTTGCGCTTCGCCGAG
GCGACGGGGAGCAAGTTTCACGTTCGCCTCTCTGCGGCTTGA

Structures

Antitoxin

• AlphaFold DB: A0A1A6FQK6