Detailed information of TA system TA085972

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 29433..29703
• Replicon: plasmid pFORC64.2
• Accession: NZ_CP023733
• Organism: Escherichia coli strain FORC 064

Toxin (Protein)

• Gene name: hok
• Uniprot ID: P11895
• Locus tag: FORC64_RS26355
• Protein ID: WP_001312861.1
• Coordinates: 29545..29703 (+)
• Length: 53 a.a.

Antitoxin (RNA)

Gene name	sok
Locus tag	-
Coordinates	29433..29496 (-)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
FORC64_RS26330	25144..25671	+	528	WP_000290834.1	single-stranded DNA-binding protein	-
FORC64_RS26335	25729..25962	+	234	WP_000006003.1	DUF905 family protein	-
FORC64_RS26340	26023..28046	+	2024	Protein_37	ParB/RepB/Spo0J family partition protein	-
FORC64_RS26345	28115..28549	+	435	WP_000845953.1	conjugation system SOS inhibitor PsiB	-
FORC64_RS26350	28546..29265	+	720	WP_001276217.1	plasmid SOS inhibition protein A	-
-	29277..29501	+	225	NuclAT_0	-	-
-	29277..29501	+	225	NuclAT_0	-	-
-	29277..29501	+	225	NuclAT_0	-	-
-	29277..29501	+	225	NuclAT_0	-	-
FORC64_RS27340	29286..29465	-	180	WP_001309233.1	hypothetical protein	-
-	29433..29496	-	64	-	-	Antitoxin
FORC64_RS26355	29545..29703	+	159	WP_001312861.1	type I toxin-antitoxin system Hok family toxin	Toxin
FORC64_RS27460	29941..30318	-	378	Protein_42	hypothetical protein	-
FORC64_RS27465	30388..30594	+	207	WP_000547974.1	hypothetical protein	-
FORC64_RS26370	30618..30914	+	297	WP_001272251.1	hypothetical protein	-
FORC64_RS26375	31025..31846	+	822	WP_001234445.1	DUF945 domain-containing protein	-
FORC64_RS26380	32143..32745	-	603	WP_000243713.1	transglycosylase SLT domain-containing protein	-
FORC64_RS26390	33068..33451	+	384	WP_001354030.1	relaxosome protein TraM	-
FORC64_RS26395	33645..34316	+	672	WP_000283561.1	conjugal transfer transcriptional regulator TraJ	-
FORC64_RS26400	34453..34680	+	228	WP_000089263.1	conjugal transfer relaxosome protein TraY	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	qnrS1	-	1..70107	70107

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (8-48)

Sequences

Toxin

Protein

• Length: 53 a.a.
• Molecular weight: 6082.32 Da
• Isoelectric Point: 9.2584

>T85972 WP_001312861.1 NZ_CP023733:29545-29703 [Escherichia coli]
MKLPRSSLVWCVLIVCLTLLIFTYLTRKSLCEIRYRDGHREVAAFMAYESGK

Nucleotide

• Length: 159 bp

>T85972 NZ_CP023733:29545-29703 [Escherichia coli]
ATGAAACTACCACGAAGTTCCCTTGTCTGGTGTGTGTTGATCGTGTGTCTCACACTGTTGATATTCACTTATCTGACACG
AAAATCGCTGTGCGAGATTCGTTACAGAGACGGACACAGGGAGGTGGCGGCTTTCATGGCTTACGAATCCGGTAAGTAG

Antitoxin

Nucleotide

• Length: 64 bp

>AT85972 NZ_CP023733:c29496-29433 [Escherichia coli]
GACTAGACATAGGGATGCCTCGTGGTGGTTAATGAAAATTAACTTACTACGGGGCTATCTTCTT

Structures

Toxin

• AlphaFold DB: P11895

Antitoxin