Detailed information of TA system    

insolicoBioinformatically predicted

Overview


TA module


Type I Classification (family/domain) hok-sok/-
Location 55337..55590 Replicon plasmid unnamed3
Accession NZ_CP023725
Organism Klebsiella pneumoniae strain TVGHCRE225

Toxin (Protein)


Gene name srnB Uniprot ID G9G1E3
Locus tag CO233_RS31300 Protein ID WP_001312851.1
Coordinates 55441..55590 (+) Length 50 a.a.

Antitoxin (RNA)


Gene name srnC
Locus tag -
Coordinates 55337..55396 (-)

Genomic Context


Locus tag Coordinates Strand Size (bp) Protein ID Product Description
CO233_RS33300 50479..50940 - 462 WP_014343465.1 hypothetical protein -
CO233_RS31270 50897..51127 + 231 WP_001261282.1 AbrB/MazE/SpoVT family DNA-binding domain-containing protein -
CO233_RS31275 51124..51540 + 417 WP_001044770.1 type II toxin-antitoxin system VapC family toxin -
CO233_RS31280 51614..53176 + 1563 WP_004206609.1 AAA family ATPase -
CO233_RS31285 53161..54183 + 1023 WP_000361404.1 helicase UvrD -
CO233_RS31290 54439..55136 + 698 Protein_73 IS1 family transposase -
- 55337..55396 - 60 NuclAT_0 - Antitoxin
- 55337..55396 - 60 NuclAT_0 - Antitoxin
- 55337..55396 - 60 NuclAT_0 - Antitoxin
- 55337..55396 - 60 NuclAT_0 - Antitoxin
CO233_RS31300 55441..55590 + 150 WP_001312851.1 type I toxin-antitoxin system Hok family toxin Toxin
CO233_RS31305 55874..56122 + 249 WP_000083837.1 replication regulatory protein RepA -
CO233_RS31315 56367..56441 + 75 WP_001375168.1 RepA leader peptide Tap -
CO233_RS31320 56434..57291 + 858 WP_032495102.1 incFII family plasmid replication initiator RepA -
CO233_RS31330 58230..58883 + 654 WP_000616807.1 CPBP family intramembrane metalloprotease -
CO233_RS31820 58976..59233 + 258 WP_000557619.1 type II toxin-antitoxin system antitoxin PemI -
CO233_RS31335 59166..59567 + 402 WP_001398199.1 type II toxin-antitoxin system toxin endoribonuclease PemK -
CO233_RS31350 59816..60231 + 416 Protein_81 IS1 family transposase -

Associated MGEs


MGE
detail
Similar
MGEs
Relative
position
MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid blaKPC-2 - 1..103454 103454
- flank IS/Tn - - 54633..55136 503


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains


Predicted by InterproScan

Toxin

(4-45)


Sequences


Toxin        


Download         Length: 50 a.a.        Molecular weight: 5542.67 Da        Isoelectric Point: 8.7678

>T85964 WP_001312851.1 NZ_CP023725:55441-55590 [Klebsiella pneumoniae]
MTKYALIGLLAVCATVLCFSLIFRERLCELNIHRGNTVVQVTLAYEARK

Download         Length: 150 bp

>T85964 NZ_CP023725:55441-55590 [Klebsiella pneumoniae]
ATGACGAAATATGCCCTTATCGGGTTGCTCGCCGTGTGTGCCACGGTATTGTGTTTTTCACTGATATTCAGGGAACGGTT
ATGTGAACTGAATATTCACAGGGGAAATACAGTGGTGCAGGTAACTCTGGCCTACGAAGCACGGAAGTAA

Antitoxin


Download         Length: 60 bp

>AT85964 NZ_CP023725:c55396-55337 [Klebsiella pneumoniae]
TAAGTACTTCATGCAGACCTCACGAGTTAATGGATTAACGAGTGGGGTCTTCGCATTTCT

Similar Proteins


Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures


Toxin

Source ID Structure
AlphaFold DB A0A829C8K5


Antitoxin

Download structure file

References