Detailed information of TA system TA085962

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/COG4683-HTH_37
• Location: 58594..59251
• Replicon: plasmid unnamed2
• Accession: NZ_CP023724
• Organism: Klebsiella pneumoniae strain TVGHCRE225

Toxin (Protein)

• Gene name: higB
• Uniprot ID: U3PDC3
• Locus tag: CO233_RS30230
• Protein ID: WP_000270043.1
• Coordinates: 58901..59251 (-)
• Length: 117 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: CO233_RS30225
• Protein ID: WP_000124640.1
• Coordinates: 58594..58896 (-)
• Length: 101 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CO233_RS30175	54219..54647	+	429	WP_000591074.1	hypothetical protein	-
CO233_RS30180	54704..55063	+	360	WP_000422768.1	hypothetical protein	-
CO233_RS30185	55063..55509	+	447	WP_000919345.1	Fe3+-siderophore ABC transporter permease	-
CO233_RS30190	55506..56024	+	519	WP_000210756.1	nitrite reductase	-
CO233_RS30195	56024..56254	+	231	WP_000972663.1	hypothetical protein	-
CO233_RS30200	56241..57098	+	858	WP_001167032.1	hypothetical protein	-
CO233_RS30210	57329..57856	+	528	WP_001236377.1	thermonuclease family protein	-
CO233_RS30215	57914..58186	+	273	WP_001043047.1	HU family DNA-binding protein	-
CO233_RS30220	58274..58567	+	294	WP_001239997.1	chromosome segregation protein ParM	-
CO233_RS30225	58594..58896	-	303	WP_000124640.1	XRE family transcriptional regulator	Antitoxin
CO233_RS30230	58901..59251	-	351	WP_000270043.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
CO233_RS30235	59414..59962	+	549	WP_001061574.1	transcriptional regulator	-
CO233_RS30245	60303..60497	+	195	WP_000343597.1	hypothetical protein	-
CO233_RS30250	60508..60879	+	372	WP_000516916.1	hypothetical protein	-
CO233_RS30255	60872..61342	+	471	WP_001281821.1	hypothetical protein	-
CO233_RS30260	61357..61692	-	336	WP_000683476.1	hypothetical protein	-
CO233_RS30265	61789..62277	+	489	WP_001273096.1	DUF1643 domain-containing protein	-
CO233_RS30270	62280..62777	+	498	WP_000062185.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	dfrA12 / aadA2 / qacE / sul1 / qnrA1 / aac(3)-IId / blaTEM-1B / aph(6)-Id / aph(3'')-Ib / sul2	-	1..159072	159072

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-like (12-106)

Antitoxin

• Cro/C1-type helix-turn-helix domain (33-79)

Sequences

Toxin

Protein

• Length: 117 a.a.
• Molecular weight: 13328.21 Da
• Isoelectric Point: 5.2421

>T85962 WP_000270043.1 NZ_CP023724:c59251-58901 [Klebsiella pneumoniae]
MWVIETTDTFDEWFDALDDTDRANVLASMMVLRDRGPMLSRPYADTVNGSSYSNMKELRVQSKGDPIRAFFAFDPKRKGI
LLCAGNKTGDEKRFYEVMIPIADREFAAHLDKLKKE

Nucleotide

• Length: 351 bp

>T85962 NZ_CP023724:c59251-58901 [Klebsiella pneumoniae]
ATGTGGGTCATCGAGACAACCGACACCTTTGATGAGTGGTTCGATGCTCTGGATGATACCGATAGAGCAAACGTGCTGGC
TTCGATGATGGTGCTGCGAGATAGAGGCCCCATGCTGTCGAGGCCATACGCGGATACTGTTAACGGTTCATCCTACAGCA
ACATGAAAGAGCTTCGGGTCCAAAGCAAAGGAGATCCTATCAGAGCGTTCTTTGCGTTCGATCCAAAGCGTAAGGGGATT
CTTCTCTGCGCCGGTAACAAGACCGGGGACGAGAAAAGGTTTTATGAAGTAATGATCCCAATTGCAGACCGTGAGTTTGC
GGCGCACTTGGATAAATTGAAGAAGGAGTGA

Antitoxin

Protein

• Length: 101 a.a.
• Molecular weight: 11006.73 Da
• Isoelectric Point: 7.2036

>AT85962 WP_000124640.1 NZ_CP023724:c58896-58594 [Klebsiella pneumoniae]
MARTLDQMLATEKPEVVAKAQKAATEMLLNIHLAELRDRMNLTQGEIAASLGVRQPTVSEMEKPGRDLKLSSIKRYVEAS
GGKLRLDVELPDGTHYGFAV

Nucleotide

• Length: 303 bp

>AT85962 NZ_CP023724:c58896-58594 [Klebsiella pneumoniae]
ATGGCAAGAACTCTTGACCAAATGCTGGCAACAGAAAAGCCTGAGGTTGTTGCCAAAGCACAGAAAGCGGCCACTGAGAT
GTTACTGAACATCCACTTGGCAGAGCTTCGTGATCGCATGAACCTTACCCAGGGGGAAATTGCTGCATCTCTGGGTGTGA
GACAGCCGACAGTCTCCGAGATGGAAAAACCTGGACGAGATCTCAAGCTGTCGTCCATTAAGCGGTACGTTGAGGCCTCC
GGTGGCAAGCTACGCCTCGATGTTGAACTTCCGGACGGCACCCACTACGGCTTTGCCGTTTAA

Structures

Toxin

• AlphaFold DB: A0A7G8AFB9