Detailed information of TA system TA085921

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/Tad-HTH_37
• Location: 3408094..3408721
• Replicon: chromosome
• Accession: NZ_CP023706
• Organism: Edwardsiella tarda strain KC-Pc-HB1

Toxin (Protein)

• Gene name: higB
• Uniprot ID: A0A411H7J1
• Locus tag: CPU03_RS15925
• Protein ID: WP_015871332.1
• Coordinates: 3408392..3408721 (-)
• Length: 110 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: CPU03_RS15920
• Protein ID: WP_005286394.1
• Coordinates: 3408094..3408402 (-)
• Length: 103 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CPU03_RS15895	3403868..3405250	+	1383	WP_097364612.1	replicative DNA helicase	-
CPU03_RS15900	3405278..3405625	+	348	WP_097364613.1	hypothetical protein	-
CPU03_RS15905	3405735..3406379	+	645	WP_097364614.1	DUF1367 family protein	-
CPU03_RS15910	3406382..3406687	+	306	WP_035603599.1	DUF1364 domain-containing protein	-
CPU03_RS17470	3406749..3407078	+	330	WP_146196788.1	hypothetical protein	-
CPU03_RS17475	3407102..3407374	+	273	WP_157757877.1	hypothetical protein	-
CPU03_RS15915	3407487..3407999	-	513	WP_097364615.1	DUF2442 domain-containing protein	-
CPU03_RS15920	3408094..3408402	-	309	WP_005286394.1	helix-turn-helix transcriptional regulator	Antitoxin
CPU03_RS15925	3408392..3408721	-	330	WP_015871332.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
CPU03_RS15930	3409166..3409936	+	771	WP_097364616.1	Rha family transcriptional regulator	-
CPU03_RS15935	3410118..3411413	+	1296	WP_157757878.1	hypothetical protein	-
CPU03_RS15940	3411535..3412353	+	819	WP_097364618.1	hypothetical protein	-
CPU03_RS17480	3412587..3412985	+	399	WP_157757879.1	hypothetical protein	-
CPU03_RS15945	3413102..3413332	+	231	WP_015871327.1	ParD-like family protein	-
CPU03_RS15950	3413307..3413636	+	330	WP_035603613.1	type II toxin-antitoxin system RelE/ParE family toxin	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	-	3376033..3447312	71279

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-like (16-103)

Antitoxin

• Cro/C1-type helix-turn-helix domain (44-97)

Sequences

Toxin

Protein

• Length: 110 a.a.
• Molecular weight: 12771.78 Da
• Isoelectric Point: 9.6247

>T85921 WP_015871332.1 NZ_CP023706:c3408721-3408392 [Edwardsiella tarda]
MNYTIEYYSEEVRLEVDQLPMGMRVRYQHLVERMEIYGSNLGEPHTSPFGDGLFELRIKGSDGIARVFYCTLTGKRIVML
HSFIKKTQKTPSAERKKAETRMKEVKHGW

Nucleotide

• Length: 330 bp

>T85921 NZ_CP023706:c3408721-3408392 [Edwardsiella tarda]
ATGAACTACACTATTGAGTACTACAGCGAAGAGGTTCGGCTTGAGGTCGATCAGTTGCCAATGGGTATGCGGGTTCGATA
CCAGCATCTCGTTGAACGCATGGAAATCTACGGTAGTAATCTCGGAGAACCACACACAAGCCCTTTTGGCGATGGGCTTT
TTGAGCTTCGGATCAAAGGCAGTGATGGCATCGCCCGCGTGTTTTACTGCACTCTTACCGGAAAACGTATCGTCATGCTG
CACAGCTTCATCAAGAAAACCCAGAAGACGCCAAGCGCTGAACGTAAGAAGGCTGAAACCAGAATGAAGGAGGTAAAACA
TGGCTGGTAA

Antitoxin

Protein

• Length: 103 a.a.
• Molecular weight: 11233.90 Da
• Isoelectric Point: 8.5371

>AT85921 WP_005286394.1 NZ_CP023706:c3408402-3408094 [Edwardsiella tarda]
MAGKRTPPTMTHDEMAAKWMEDPAFKAEYDAIEEEYALLDEMLAARKNAGLTQAQVAERMGTKPTAITRMESNLASGQSG
PSFATLKKFAHATGKKLQIRFV

Nucleotide

• Length: 309 bp

>AT85921 NZ_CP023706:c3408402-3408094 [Edwardsiella tarda]
ATGGCTGGTAAACGCACCCCCCCCACGATGACGCATGATGAAATGGCTGCAAAGTGGATGGAAGACCCGGCATTTAAAGC
AGAATACGATGCCATCGAAGAGGAATACGCCCTACTCGATGAAATGCTCGCCGCCCGCAAAAACGCGGGGCTGACCCAGG
CGCAGGTCGCCGAACGTATGGGAACCAAGCCTACAGCGATCACTCGCATGGAAAGTAATTTAGCCTCCGGCCAAAGCGGG
CCATCATTCGCCACGCTGAAAAAATTTGCACACGCGACCGGCAAGAAACTGCAGATCCGGTTTGTCTGA

Structures

Toxin

• AlphaFold DB: A0A411H7J1