Detailed information of TA system TA085901

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): DinJ-YafQ (relBE)/YafQ-RelB
• Location: 22671..23199
• Replicon: plasmid pZM41
• Accession: NZ_CP023686
• Organism: Zymomonas mobilis subsp. mobilis strain ZM4 substr. 8b

Toxin (Protein)

• Gene name: yafQ
• Uniprot ID: -
• Locus tag: ZMO3_RS09875
• Protein ID: WP_062339761.1
• Coordinates: 22671..22940 (-)
• Length: 90 a.a.

Antitoxin (Protein)

• Gene name: dinJ
• Uniprot ID: -
• Locus tag: ZMO3_RS09880
• Protein ID: WP_062339759.1
• Coordinates: 22942..23199 (-)
• Length: 86 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
ZMO3_RS09825	17751..18152	+	402	WP_161527439.1	phage tail protein	-
ZMO3_RS09830	18149..19132	+	984	WP_062339740.1	hypothetical protein	-
ZMO3_RS09840	19456..19818	-	363	WP_108125223.1	hypothetical protein	-
ZMO3_RS09845	20354..20593	-	240	WP_062339772.1	hypothetical protein	-
ZMO3_RS09850	20604..20792	-	189	WP_062339770.1	hypothetical protein	-
ZMO3_RS09860	21271..21597	-	327	WP_062339766.1	hypothetical protein	-
ZMO3_RS09865	21702..22028	-	327	WP_062339764.1	hypothetical protein	-
ZMO3_RS09870	22057..22419	-	363	WP_151457906.1	hypothetical protein	-
ZMO3_RS09875	22671..22940	-	270	WP_062339761.1	type II toxin-antitoxin system YafQ family toxin	Toxin
ZMO3_RS09880	22942..23199	-	258	WP_062339759.1	type II toxin-antitoxin system RelB/DinJ family antitoxin	Antitoxin
ZMO3_RS09885	23613..24881	+	1269	WP_108128677.1	DNA damage-inducible protein D	-
ZMO3_RS10050	25128..25304	+	177	WP_161527440.1	hypothetical protein	-
ZMO3_RS09890	25548..26009	+	462	WP_108128671.1	hypothetical protein	-
ZMO3_RS09895	26080..26334	-	255	WP_062339701.1	hypothetical protein	-
ZMO3_RS09900	26340..26801	-	462	WP_063630121.1	helix-turn-helix domain-containing protein	-
ZMO3_RS09905	26815..27150	-	336	WP_081094484.1	type II toxin-antitoxin system RelE/ParE family toxin	-
ZMO3_RS09910	27397..27867	-	471	WP_062339703.1	helix-turn-helix domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	catA1	-	1..40858	40858

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, YafQ-like toxin (6-89)

Antitoxin

• RelB antitoxin/Antitoxin DinJ (1-84)

Sequences

Toxin

Protein

• Length: 90 a.a.
• Molecular weight: 10398.16 Da
• Isoelectric Point: 10.0220

>T85901 WP_062339761.1 NZ_CP023686:c22940-22671 [Zymomonas mobilis subsp. mobilis]
VLTPIRSSKFKKDIKKLQKRGKDMEKLKKAILYLINEQALPPAYRSHVLSGDWSGYYDLHLEPDWLLLYKIADMELHLAR
TGSHSDVFK

Nucleotide

• Length: 270 bp

>T85901 NZ_CP023686:c22940-22671 [Zymomonas mobilis subsp. mobilis]
GTGCTAACGCCTATCCGATCGAGCAAATTCAAGAAAGACATAAAAAAATTACAGAAGCGAGGTAAGGATATGGAAAAATT
AAAAAAAGCCATTCTTTACCTGATTAATGAACAAGCCTTACCACCAGCCTACCGAAGCCATGTTTTAAGTGGCGATTGGT
CGGGTTACTACGATTTGCATTTGGAACCTGACTGGCTCCTGCTCTATAAAATAGCTGATATGGAATTGCATCTAGCGCGA
ACAGGCAGCCATTCAGATGTTTTTAAGTGA

Antitoxin

Protein

• Length: 86 a.a.
• Molecular weight: 9372.01 Da
• Isoelectric Point: 8.6589

>AT85901 WP_062339759.1 NZ_CP023686:c23199-22942 [Zymomonas mobilis subsp. mobilis]
MKADSVVRARIPTLTKEAAIKTLEQMGLSISDAIRLLMTKIANEQRLPFSVELPTVETKKAIIELENGKGKTFKNADALF
EDLGI

Nucleotide

• Length: 258 bp

>AT85901 NZ_CP023686:c23199-22942 [Zymomonas mobilis subsp. mobilis]
ATGAAAGCAGATTCTGTTGTTAGAGCACGCATCCCAACCTTGACCAAGGAAGCCGCGATTAAAACTTTGGAGCAGATGGG
ACTTAGTATTTCTGATGCTATCCGGTTGCTTATGACTAAAATCGCCAATGAGCAACGTCTTCCCTTTTCTGTTGAATTAC
CAACGGTGGAAACCAAAAAAGCGATTATCGAATTGGAAAATGGAAAAGGTAAGACTTTTAAAAATGCAGATGCTCTGTTC
GAGGATTTAGGTATTTAA