Detailed information of TA system TA085889

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 65821..66075
• Replicon: plasmid pO177A2
• Accession: NZ_CP023674
• Organism: Escherichia coli strain SMN013SH2

Toxin (Protein)

• Gene name: srnB
• Uniprot ID: -
• Locus tag: CP957_RS28345
• Protein ID: WP_001351576.1
• Coordinates: 65869..66075 (+)
• Length: 69 a.a.

Antitoxin (RNA)

Gene name	srnC
Locus tag	-
Coordinates	65821..65882 (-)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CP957_RS28325	63536..64282	+	747	WP_062881715.1	conjugal transfer pilus acetylase TraX	-
CP957_RS28330	64337..64897	+	561	WP_101974184.1	fertility inhibition protein FinO	-
CP957_RS28335	65033..65245	+	213	WP_001299730.1	ANR family transcriptional regulator	-
CP957_RS29555	65491..65565	+	75	Protein_65	endonuclease	-
-	65821..65882	-	62	NuclAT_0	-	Antitoxin
-	65821..65882	-	62	NuclAT_0	-	Antitoxin
-	65821..65882	-	62	NuclAT_0	-	Antitoxin
-	65821..65882	-	62	NuclAT_0	-	Antitoxin
CP957_RS28345	65869..66075	+	207	WP_001351576.1	type I toxin-antitoxin system Hok family toxin	Toxin
CP957_RS28350	66359..66616	+	258	WP_000083845.1	replication regulatory protein RepA	-
CP957_RS28360	66848..66922	+	75	WP_001442103.1	RepA leader peptide Tap	-
CP957_RS28365	66915..67397	+	483	WP_001523473.1	hypothetical protein	-
CP957_RS28370	67390..68247	+	858	WP_096851680.1	incFII family plasmid replication initiator RepA	-
CP957_RS28380	69158..69442	+	285	WP_062881718.1	ribbon-helix-helix domain-containing protein	-
CP957_RS28385	69442..69717	+	276	WP_000421257.1	type II toxin-antitoxin system RelE/ParE family toxin	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	espP / hlyD / hlyB / hlyA / hlyC	1..87524	87524

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (23-64)

Sequences

Toxin

Protein

• Length: 69 a.a.
• Molecular weight: 7826.31 Da
• Isoelectric Point: 8.8807

>T85889 WP_001351576.1 NZ_CP023674:65869-66075 [Escherichia coli]
MKYLNTTDCSLFFAERSKFMTKYALIGLLAVCATVLCFSLIFRERLCELNIHRGNTVVQVTLAYEARK

Nucleotide

• Length: 207 bp

>T85889 NZ_CP023674:65869-66075 [Escherichia coli]
ATGAAGTACCTTAACACTACTGATTGTAGCCTCTTCTTTGCTGAGAGGTCAAAGTTTATGACGAAATATGCCCTTATCGG
GTTGCTCGCCGTGTGCGCCACGGTGTTGTGTTTTTCACTGATATTCAGGGAACGGTTATGTGAGCTGAATATTCACAGAG
GAAATACAGTGGTGCAGGTAACTCTGGCCTACGAAGCACGGAAGTAA

Antitoxin

Nucleotide

• Length: 62 bp

>AT85889 NZ_CP023674:c65882-65821 [Escherichia coli]
TTAAGGTACTTCATGCAGACCTCACGAGTTAATGGATTAACGAGTGGGGTCTTCGCATTTCT

Structures

Antitoxin