Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) DinJ-YafQ (relBE)/YafQ-RelB
Location 3998960..3999501 Replicon chromosome
Accession NZ_CP023673
Organism Escherichia coli strain SMN013SH2

Toxin (Protein)


Gene name yafQ Uniprot ID S1FHQ9
Locus tag CP957_RS21990 Protein ID WP_000615979.1
Coordinates 3999223..3999501 (+) Length 93 a.a.

Antitoxin (Protein)


Gene name dinJ Uniprot ID S1EWQ0
Locus tag CP957_RS21985 Protein ID WP_000729704.1
Coordinates 3998960..3999220 (+) Length 87 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
CP957_RS21950 3993982..3994596 - 615 WP_062882562.1 peptide chain release factor H -
CP957_RS21955 3994593..3995734 - 1142 Protein_4023 RNA ligase RtcB family protein -
CP957_RS21965 3995980..3996432 - 453 WP_071998501.1 GNAT family N-acetyltransferase -
CP957_RS21970 3996429..3997484 - 1056 WP_001226164.1 DNA polymerase IV -
CP957_RS21975 3997555..3998340 - 786 WP_062882563.1 putative lateral flagellar export/assembly protein LafU -
CP957_RS21980 3998312..3998673 + 362 Protein_4027 FHIPEP family type III secretion protein -
CP957_RS29475 3998664..3998774 - 111 Protein_4028 peptidoglycan endopeptidase -
CP957_RS21985 3998960..3999220 + 261 WP_000729704.1 type II toxin-antitoxin system antitoxin DinJ Antitoxin
CP957_RS21990 3999223..3999501 + 279 WP_000615979.1 type II toxin-antitoxin system mRNA interferase toxin YafQ Toxin
CP957_RS21995 3999657..4000397 + 741 WP_001225679.1 murein L,D-transpeptidase -
CP957_RS22000 4000368..4001135 - 768 WP_001365365.1 class II glutamine amidotransferase -
CP957_RS22005 4001341..4001919 - 579 WP_062882565.1 D-sedoheptulose 7-phosphate isomerase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, YafQ-like toxin

(1-91)

Antitoxin

RelB antitoxin/Antitoxin DinJ

(4-84)


Sequences

Toxin        


Download         Length: 93 a.a.        Molecular weight: 10830.62 Da        Isoelectric Point: 10.0702

>T85885 WP_000615979.1 NZ_CP023673:3999223-3999501 [Escherichia coli]
MIQRDIEYSGQFSKDVKLAQKRHKDMNKLKYLMTLLINNTLPLPAVYKDHPLQGSWKGYRDAHVEPDWILIYKLTDKLLR
FERTGTHAALFG

Download         Length: 279 bp

>T85885 NZ_CP023673:3999223-3999501 [Escherichia coli]
ATGATTCAAAGGGATATTGAATACTCGGGACAATTTTCAAAGGATGTAAAACTTGCACAAAAGCGTCATAAGGATATGAA
TAAATTGAAATATCTTATGACGCTTCTTATCAATAATACTTTACCGCTTCCAGCTGTTTATAAAGACCACCCGCTGCAAG
GTTCATGGAAAGGTTATCGCGATGCTCATGTCGAACCGGACTGGATCCTGATTTACAAACTTACCGATAAACTTTTACGA
TTTGAGAGAACTGGAACTCACGCGGCGCTCTTTGGGTAA

Antitoxin


Download         Length: 87 a.a.        Molecular weight: 9391.77 Da        Isoelectric Point: 5.0758

>AT85885 WP_000729704.1 NZ_CP023673:3998960-3999220 [Escherichia coli]
MAANAFVRARIDEDLKNQAADVLAGMGLTISDLVRITLTKVAREKALPFDLREPNQLTIQSIKNSEAGVDVHKAKDADDL
FDKLGI

Download         Length: 261 bp

>AT85885 NZ_CP023673:3998960-3999220 [Escherichia coli]
ATGGCTGCTAACGCATTTGTTCGCGCCCGAATCGATGAAGATCTGAAGAATCAGGCGGCGGACGTACTGGCCGGGATGGG
GCTGACCATCTCTGACCTGGTTCGCATAACCCTCACAAAGGTCGCGCGTGAAAAGGCATTGCCGTTTGATTTACGCGAGC
CTAATCAATTAACCATTCAATCAATCAAAAACAGCGAAGCTGGCGTTGATGTTCATAAGGCCAAAGACGCCGATGATTTA
TTTGATAAATTAGGAATTTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB S1FHQ9

Antitoxin

Source ID Structure
PDB 4ML0
AlphaFold DB A0A0E0Y6Z6

References