Detailed information of TA system TA085868

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-HigA
• Location: 1050619..1051346
• Replicon: chromosome
• Accession: NZ_CP023673
• Organism: Escherichia coli strain SMN013SH2

Toxin (Protein)

• Gene name: higB
• Uniprot ID: Q3YYE6
• Locus tag: CP957_RS05575
• Protein ID: WP_000547563.1
• Coordinates: 1050619..1050930 (+)
• Length: 104 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: CP957_RS05580
• Protein ID: WP_000126296.1
• Coordinates: 1050927..1051346 (+)
• Length: 140 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CP957_RS05545	1045765..1047474	+	1710	WP_062881985.1	formate hydrogenlyase subunit HycE	-
CP957_RS05550	1047484..1048026	+	543	WP_000493785.1	formate hydrogenlyase subunit HycF	-
CP957_RS05555	1048026..1048793	+	768	WP_000067403.1	formate hydrogenlyase subunit HycG	-
CP957_RS05560	1048790..1049200	+	411	WP_001291918.1	formate hydrogenlyase assembly protein HycH	-
CP957_RS05565	1049193..1049663	+	471	WP_000132961.1	hydrogenase maturation peptidase HycI	-
CP957_RS05570	1049706..1050457	+	752	Protein_1012	hypothetical protein	-
CP957_RS05575	1050619..1050930	+	312	WP_000547563.1	type II toxin-antitoxin system HigB family toxin	Toxin
CP957_RS05580	1050927..1051346	+	420	WP_000126296.1	helix-turn-helix domain-containing protein	Antitoxin
CP957_RS05585	1051460..1052884	-	1425	WP_024226292.1	6-phospho-beta-glucosidase AscB	-
CP957_RS05590	1052893..1054350	-	1458	WP_001107859.1	PTS cellobiose/arbutin/salicin transporter subunit IIBC	-
CP957_RS05595	1054610..1055620	+	1011	WP_001343660.1	DNA-binding transcriptional regulator AscG	-
CP957_RS05600	1055769..1056296	+	528	WP_001078777.1	electron transport protein HydN	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, mRNA interferase HigB (22-93)

Antitoxin

• Cro/C1-type helix-turn-helix domain (85-138)

Sequences

Toxin

Protein

• Length: 104 a.a.
• Molecular weight: 12398.11 Da
• Isoelectric Point: 9.7105

>T85868 WP_000547563.1 NZ_CP023673:1050619-1050930 [Escherichia coli]
MHIISKAPFEESARKYPNDALALQALYRVIKETDFSTPEEMRTAFPNLDNFKYRNKWYVLDVGGNNLRVIAYINFVNKRF
FVKHITNHAEYDKLTRYYRENKE

Nucleotide

• Length: 312 bp

>T85868 NZ_CP023673:1050619-1050930 [Escherichia coli]
ATGCACATCATTTCTAAAGCGCCATTTGAAGAGTCTGCGCGTAAATATCCTAACGACGCTCTGGCTCTTCAGGCATTGTA
TCGGGTTATTAAGGAAACGGATTTTTCTACGCCAGAAGAGATGCGAACGGCGTTTCCCAACCTCGACAATTTTAAATATC
GCAACAAATGGTACGTTCTGGATGTAGGTGGGAACAACCTAAGGGTGATTGCCTACATAAACTTTGTTAATAAGCGGTTC
TTTGTGAAGCACATAACTAATCACGCCGAGTATGACAAACTGACACGTTACTACAGAGAGAACAAAGAATGA

Antitoxin

Protein

• Length: 140 a.a.
• Molecular weight: 15416.38 Da
• Isoelectric Point: 4.3653

>AT85868 WP_000126296.1 NZ_CP023673:1050927-1051346 [Escherichia coli]
MTANAARAVKATRELVNAVPFLGGSDSEDDYREALELVEYLIEEDDTNPLIDFLASRIAEYENNNQEFAEFDKAVAAMPV
GVALLRTLIDQHNLTYADLKNEIGSKSLVSQILSGQRSLTISHIKALSARFGVKPEWFL

Nucleotide

• Length: 420 bp

>AT85868 NZ_CP023673:1050927-1051346 [Escherichia coli]
ATGACTGCTAATGCTGCACGCGCTGTTAAAGCAACAAGAGAACTGGTCAATGCAGTCCCCTTTCTGGGAGGTAGTGACTC
AGAAGATGATTATCGTGAAGCACTGGAACTGGTCGAATATTTGATTGAAGAAGATGACACCAATCCACTTATCGACTTTC
TGGCGAGCCGCATTGCAGAATATGAAAACAACAACCAAGAGTTTGCAGAGTTCGACAAAGCCGTTGCGGCAATGCCTGTT
GGCGTGGCGTTACTTCGCACGCTGATTGATCAACATAATCTGACCTATGCGGATTTGAAGAACGAAATCGGCTCTAAATC
CCTGGTCAGTCAGATTTTGTCTGGACAACGATCCCTGACGATATCCCACATTAAGGCACTTTCAGCCCGATTTGGCGTTA
AGCCGGAATGGTTTCTTTGA

Structures

Toxin

• AlphaFold DB: A0A7U9QA22