Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) DinJ-YafQ (relBE)/YafQ-RelB
Location 3528367..3528908 Replicon chromosome
Accession NZ_CP023645
Organism Shigella sonnei strain CFSAN030807

Toxin (Protein)


Gene name yafQ Uniprot ID U9XPG0
Locus tag AT852_RS19720 Protein ID WP_000615976.1
Coordinates 3528630..3528908 (+) Length 93 a.a.

Antitoxin (Protein)


Gene name dinJ Uniprot ID S1EWQ0
Locus tag AT852_RS19715 Protein ID WP_000729704.1
Coordinates 3528367..3528627 (+) Length 87 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
AT852_RS19690 (3523836) 3523836..3525548 + 1713 Protein_3537 flagellar biosynthesis protein FlhA -
AT852_RS19695 (3525671) 3525671..3526168 - 498 WP_000006258.1 REP-associated tyrosine transposase RayT -
AT852_RS28345 (3526239) 3526239..3526367 - 129 Protein_3539 ISAs1 family transposase -
AT852_RS19705 (3526422) 3526422..3527119 + 698 WP_094096600.1 IS1-like element IS1A family transposase -
AT852_RS19710 (3527408) 3527408..3528181 - 774 WP_127148796.1 C40 family peptidase -
AT852_RS19715 (3528367) 3528367..3528627 + 261 WP_000729704.1 type II toxin-antitoxin system antitoxin DinJ Antitoxin
AT852_RS19720 (3528630) 3528630..3528908 + 279 WP_000615976.1 type II toxin-antitoxin system mRNA interferase toxin YafQ Toxin
AT852_RS19725 (3529064) 3529064..3529804 + 741 WP_001225679.1 peptidoglycan meso-diaminopimelic acid protein amidase -
AT852_RS19730 (3529775) 3529775..3530542 - 768 WP_000333380.1 class II glutamine amidotransferase -
AT852_RS19735 (3530748) 3530748..3531326 - 579 WP_000284050.1 D-sedoheptulose 7-phosphate isomerase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Genomic island - gmhA/lpcA 3526795..3539769 12974


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, YafQ-like toxin

(1-91)

Antitoxin

RelB antitoxin/Antitoxin DinJ

(4-84)


Sequences

Toxin        


Download         Length: 93 a.a.        Molecular weight: 10800.59 Da        Isoelectric Point: 10.0702

>T85816 WP_000615976.1 NZ_CP023645:3528630-3528908 [Shigella sonnei]
MIQRDIEYSGQFSKDVKLAQKRHKDMNKLKYLMTLLINNALPLPAVYKDHPLQGSWKGYRDAHVEPDWILIYKLTDKLLR
FERTGTHAALFG

Download         Length: 279 bp

>T85816 NZ_CP023645:3528630-3528908 [Shigella sonnei]
ATGATTCAAAGGGATATTGAATACTCGGGACAATTTTCAAAGGATGTAAAACTTGCACAAAAGCGTCATAAGGATATGAA
TAAATTGAAATATCTTATGACGCTTCTTATCAATAATGCTTTACCGCTTCCAGCTGTTTATAAAGACCACCCGCTGCAAG
GTTCATGGAAAGGTTATCGCGATGCTCATGTCGAACCGGACTGGATCCTGATTTACAAACTTACCGATAAACTTTTACGA
TTTGAGAGAACTGGAACTCACGCGGCGCTCTTTGGGTAA

Antitoxin


Download         Length: 87 a.a.        Molecular weight: 9391.77 Da        Isoelectric Point: 5.0758

>AT85816 WP_000729704.1 NZ_CP023645:3528367-3528627 [Shigella sonnei]
MAANAFVRARIDEDLKNQAADVLAGMGLTISDLVRITLTKVAREKALPFDLREPNQLTIQSIKNSEAGVDVHKAKDADDL
FDKLGI

Download         Length: 261 bp

>AT85816 NZ_CP023645:3528367-3528627 [Shigella sonnei]
ATGGCTGCTAACGCGTTTGTTCGCGCCCGAATCGATGAAGATCTGAAGAATCAGGCGGCGGACGTACTGGCCGGGATGGG
GCTGACCATCTCTGACCTGGTTCGCATAACCCTCACAAAGGTCGCGCGTGAAAAGGCATTGCCGTTTGATTTACGCGAGC
CTAATCAATTAACCATTCAATCAATCAAAAACAGCGAAGCTGGCGTTGATGTTCATAAGGCCAAAGACGCTGATGATTTA
TTTGATAAATTAGGAATTTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB U9XPG0

Antitoxin

Source ID Structure
PDB 4ML0
AlphaFold DB A0A0E0Y6Z6

References