Detailed information of TA system TA085803

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-HigA
• Location: 818955..819682
• Replicon: chromosome
• Accession: NZ_CP023645
• Organism: Shigella sonnei strain CFSAN030807

Toxin (Protein)

• Gene name: higB
• Uniprot ID: Q3YYE6
• Locus tag: AT852_RS04510
• Protein ID: WP_000547563.1
• Coordinates: 818955..819266 (+)
• Length: 104 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: AT852_RS04515
• Protein ID: WP_000126296.1
• Coordinates: 819263..819682 (+)
• Length: 140 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
AT852_RS04480 (814109)	814109..815806	+	1698	WP_001288143.1	formate hydrogenlyase subunit HycE	-
AT852_RS04485 (815816)	815816..816358	+	543	WP_000493785.1	formate hydrogenlyase subunit HycF	-
AT852_RS04490 (816358)	816358..817125	+	768	WP_000067403.1	formate hydrogenlyase subunit HycG	-
AT852_RS04495 (817122)	817122..817532	+	411	WP_001291918.1	formate hydrogenlyase assembly protein HycH	-
AT852_RS04500 (817525)	817525..817995	+	471	WP_000132961.1	hydrogenase maturation peptidase HycI	-
AT852_RS04505 (818020)	818020..818793	+	774	WP_001026444.1	hypothetical protein	-
AT852_RS04510 (818955)	818955..819266	+	312	WP_000547563.1	type II toxin-antitoxin system HigB family toxin	Toxin
AT852_RS04515 (819263)	819263..819682	+	420	WP_000126296.1	helix-turn-helix domain-containing protein	Antitoxin
AT852_RS04520 (819796)	819796..821220	-	1425	WP_000110319.1	6-phospho-beta-glucosidase AscB	-
AT852_RS04525 (821229)	821229..822686	-	1458	WP_001107890.1	PTS cellobiose/arbutin/salicin transporter subunit IIBC	-
AT852_RS04530 (822946)	822946..823956	+	1011	WP_001402444.1	DNA-binding transcriptional regulator AscG	-
AT852_RS04535 (824104)	824104..824631	+	528	WP_001078766.1	electron transport protein HydN	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, mRNA interferase HigB (22-93)

Antitoxin

• Cro/C1-type helix-turn-helix domain (85-138)

Sequences

Toxin

Protein

• Length: 104 a.a.
• Molecular weight: 12398.11 Da
• Isoelectric Point: 9.7105

>T85803 WP_000547563.1 NZ_CP023645:818955-819266 [Shigella sonnei]
MHIISKAPFEESARKYPNDALALQALYRVIKETDFSTPEEMRTAFPNLDNFKYRNKWYVLDVGGNNLRVIAYINFVNKRF
FVKHITNHAEYDKLTRYYRENKE

Nucleotide

• Length: 312 bp

>T85803 NZ_CP023645:818955-819266 [Shigella sonnei]
ATGCACATCATTTCTAAAGCGCCATTTGAAGAGTCTGCGCGTAAATATCCTAACGACGCTCTGGCTCTTCAGGCATTGTA
TCGGGTTATTAAGGAAACGGATTTTTCTACGCCAGAAGAGATGCGAACGGCGTTTCCCAACCTCGACAATTTTAAATATC
GCAACAAATGGTACGTTCTGGATGTAGGTGGGAACAACCTAAGGGTGATTGCCTACATAAACTTTGTTAATAAGCGGTTC
TTTGTGAAGCACATAACTAATCACGCCGAGTATGACAAACTGACACGTTACTACAGAGAGAACAAAGAATGA

Antitoxin

Protein

• Length: 140 a.a.
• Molecular weight: 15416.38 Da
• Isoelectric Point: 4.3653

>AT85803 WP_000126296.1 NZ_CP023645:819263-819682 [Shigella sonnei]
MTANAARAVKATRELVNAVPFLGGSDSEDDYREALELVEYLIEEDDTNPLIDFLASRIAEYENNNQEFAEFDKAVAAMPV
GVALLRTLIDQHNLTYADLKNEIGSKSLVSQILSGQRSLTISHIKALSARFGVKPEWFL

Nucleotide

• Length: 420 bp

>AT85803 NZ_CP023645:819263-819682 [Shigella sonnei]
ATGACTGCTAATGCTGCACGCGCTGTTAAAGCAACAAGAGAACTGGTCAATGCAGTCCCCTTTCTGGGAGGTAGTGACTC
AGAAGATGATTATCGTGAAGCACTGGAACTGGTCGAATATTTGATTGAAGAAGATGACACCAATCCACTTATCGACTTTC
TGGCGAGCCGCATTGCAGAATATGAAAACAACAACCAAGAGTTTGCAGAGTTCGACAAAGCCGTTGCGGCAATGCCTGTT
GGCGTGGCGTTACTTCGCACGCTGATTGATCAACATAATCTGACCTATGCGGATTTGAAGAACGAAATCGGCTCTAAATC
CCTGGTCAGTCAGATTTTGTCTGGACAACGATCCCTGACGATATCCCACATTAAGGCACTTTCAGCCCGATTTGGCGTTA
AGCCGGAATGGTTTCTTTGA

Structures

Toxin

• AlphaFold DB: A0A7U9QA22