Detailed information of TA system    

insolicoBioinformatically predicted

Overview


TA module


Type II Classification (family/domain) higBA (relBE)/HTH(antitoxin)
Location 2548615..2549264 Replicon chromosome
Accession NZ_CP023641
Organism Pseudomonas mendocina strain MAE1-K

Toxin (Protein)


Gene name higB Uniprot ID -
Locus tag CO724_RS12115 Protein ID WP_047588122.1
Coordinates 2548920..2549264 (-) Length 115 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID A0A396RZ86
Locus tag CO724_RS12110 Protein ID WP_014596891.1
Coordinates 2548615..2548923 (-) Length 103 a.a.

Genomic Context


Locus tag Coordinates Strand Size (bp) Protein ID Product Description
CO724_RS12095 2544374..2545480 + 1107 WP_096826295.1 trypsin-like peptidase domain-containing protein -
CO724_RS12100 2545616..2546596 - 981 WP_096825341.1 IS5-like element ISPst5 family transposase -
CO724_RS12105 2547359..2548228 + 870 WP_014596892.1 LysR family transcriptional regulator -
CO724_RS12110 2548615..2548923 - 309 WP_014596891.1 transcriptional regulator Antitoxin
CO724_RS12115 2548920..2549264 - 345 WP_047588122.1 toxin Toxin
CO724_RS12120 2549422..2550349 - 928 Protein_2406 LysR family transcriptional regulator -
CO724_RS12125 2550363..2551562 - 1200 WP_096826296.1 MFS transporter -
CO724_RS12130 2551647..2553500 - 1854 WP_096826297.1 asparagine synthase (glutamine-hydrolyzing) -

Associated MGEs


MGE
detail
Similar
MGEs
Relative
position
MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Integrative and Conjugative Element - - 2506535..2578602 72067
- flank IS/Tn - - 2545616..2546596 980


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains


Predicted by InterproScan

Toxin


No domain identified.


Antitoxin

(42-92)


Sequences


Toxin        


Download         Length: 115 a.a.        Molecular weight: 13366.33 Da        Isoelectric Point: 10.2892

>T85799 WP_047588122.1 NZ_CP023641:c2549264-2548920 [Pseudomonas mendocina]
MKALFVELSPFERNRKAHLDDDEYSLFQQMLLTNPEAGAVIANSGGLRKVRFGSVRRNKGKRGGVRVIYYYWLRGLQFWL
FTLYDKDELDDLSSDQRRQLKALLEREVKARQAP

Download         Length: 345 bp

>T85799 NZ_CP023641:c2549264-2548920 [Pseudomonas mendocina]
ATGAAAGCATTGTTTGTTGAACTGTCACCCTTTGAACGCAACCGAAAAGCCCACCTCGATGATGACGAGTACTCGCTATT
CCAGCAGATGCTCTTGACCAACCCGGAGGCAGGCGCAGTCATCGCCAACAGCGGTGGTCTGAGGAAAGTCCGATTCGGAT
CGGTCAGGCGCAACAAGGGGAAGCGAGGTGGCGTTCGGGTGATTTACTACTACTGGCTCCGCGGTCTGCAGTTCTGGCTC
TTCACCCTGTATGACAAAGACGAACTCGATGACCTGAGCAGTGACCAGCGTAGGCAGCTGAAAGCGCTCCTTGAGCGCGA
AGTGAAGGCGAGGCAAGCACCATGA

Antitoxin


Download         Length: 103 a.a.        Molecular weight: 11561.34 Da        Isoelectric Point: 10.0927

>AT85799 WP_014596891.1 NZ_CP023641:c2548923-2548615 [Pseudomonas mendocina]
MTKRDIFSELIEGFDDLAAAREGKRTLRTHKVEYTPIEPATGAEIVALREKLKMSQGVFAQAIHAKPATLKNWEQNRSKP
NDQATVLIRILNKHPDLIRELA

Download         Length: 309 bp

>AT85799 NZ_CP023641:c2548923-2548615 [Pseudomonas mendocina]
ATGACCAAACGCGATATTTTTTCCGAGCTGATCGAAGGCTTCGACGACCTGGCCGCAGCACGTGAAGGCAAGCGTACGCT
GCGTACCCACAAGGTCGAATACACGCCAATCGAGCCCGCGACAGGCGCGGAAATCGTCGCACTGCGCGAAAAGCTGAAGA
TGTCTCAGGGTGTATTCGCCCAGGCCATTCACGCCAAGCCAGCGACTCTCAAAAACTGGGAACAGAACCGCTCAAAGCCC
AACGACCAGGCTACTGTTCTCATCCGTATCCTGAACAAGCATCCGGACCTCATACGCGAACTGGCGTAG

Similar Proteins


Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures


Toxin

Source ID Structure


Antitoxin

Source ID Structure
AlphaFold DB A0A396RZ86

References