85480

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) unclassified/-
Location 2976426..2977074 Replicon chromosome
Accession NZ_CP023635
Organism Mycobacterium tuberculosis strain TBDM2489

Toxin (Protein)


Gene name novel[antitoxin] Uniprot ID P9WJ12
Locus tag CP492_RS14550 Protein ID WP_003899414.1
Coordinates 2976426..2976749 (-) Length 108 a.a.

Antitoxin (Protein)


Gene name novel[toxin] Uniprot ID P9WJ10
Locus tag CP492_RS14555 Protein ID WP_003899415.1
Coordinates 2976829..2977074 (-) Length 82 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
CP492_RS14530 2972000..2973261 + 1262 WP_087902221.1 IS3-like element IS987 family transposase -
CP492_RS14535 2973635..2975074 - 1440 WP_003899411.1 phage major capsid protein -
CP492_RS14540 2975082..2975615 - 534 WP_003899412.1 HK97 family phage prohead protease -
CP492_RS14545 2975768..2976259 - 492 WP_003900541.1 phage terminase small subunit P27 family -
CP492_RS14550 2976426..2976749 - 324 WP_003899414.1 type II toxin-antitoxin system toxin Toxin
CP492_RS14555 2976829..2977074 - 246 WP_003899415.1 type II toxin-antitoxin system antitoxin Antitoxin
CP492_RS14560 2977071..2978498 - 1428 WP_003899416.1 DUF3631 domain-containing protein -
CP492_RS14565 2978500..2978892 - 393 WP_003899417.1 DUF2742 domain-containing protein -
CP492_RS14570 2978889..2979149 - 261 WP_003899418.1 helix-turn-helix domain-containing protein -
CP492_RS14575 2979166..2979528 - 363 WP_003900543.1 hypothetical protein -
CP492_RS14580 2979531..2980658 - 1128 WP_003899420.1 site-specific integrase -
CP492_RS14585 2980803..2981030 - 228 WP_003899421.1 hypothetical protein -
CP492_RS14590 2981027..2981416 - 390 WP_003899422.1 hypothetical protein -
CP492_RS14595 2981322..2981594 + 273 WP_003900544.1 hypothetical protein -
CP492_RS14600 2981693..2981926 + 234 WP_003413717.1 hypothetical protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
inside Prophage - - 2970391..2980658 10267


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, mRNA interferase HigB

(20-92)

Antitoxin

Cro/C1-type helix-turn-helix domain

(84-135)


Sequences

Toxin        


Download         Length: 108 a.a.        Molecular weight: 12359.82 Da        Isoelectric Point: 8.6717

>T85480 WP_003899414.1 NZ_CP023635:c2976749-2976426 [Mycobacterium tuberculosis]
MTHKRTKRQPAIAAGLNAPRRNRVGRQHGWPADVPSAEQRRAQRQRDLEAIRRAYAEMVATSHEIDDDTAELALLSMHLD
DEQRRLEAGMKLGWHPYHFPDEPDSKQ

Download         Length: 324 bp

>T85480 NZ_CP023635:c2976749-2976426 [Mycobacterium tuberculosis]
TTGACCCACAAGCGCACTAAACGCCAGCCAGCCATCGCCGCAGGGCTCAACGCCCCGCGTCGGAATCGCGTTGGGCGGCA
ACATGGTTGGCCGGCCGACGTTCCGTCCGCCGAGCAGCGCCGCGCCCAACGGCAGCGCGACCTCGAGGCTATCCGCCGAG
CGTACGCCGAGATGGTGGCGACATCACACGAAATCGACGACGACACAGCCGAACTGGCGCTGTTGTCGATGCATCTCGAC
GATGAGCAGCGCCGGCTTGAGGCGGGGATGAAGCTCGGCTGGCATCCGTATCACTTCCCCGACGAACCCGACAGCAAACA
GTGA

Antitoxin


Download         Length: 82 a.a.        Molecular weight: 7697.71 Da        Isoelectric Point: 4.8565

>AT85480 WP_003899415.1 NZ_CP023635:c2977074-2976829 [Mycobacterium tuberculosis]
MSGHALAARTLLAAADELVGGPPVEASAAALAGDAAGAWRTAAVELARALVRAVAESHGVAAVLFAATAAAAAAVDRGDP
P

Download         Length: 246 bp

>AT85480 NZ_CP023635:c2977074-2976829 [Mycobacterium tuberculosis]
ATGAGCGGCCACGCGTTGGCTGCTCGGACGTTGCTGGCCGCCGCGGACGAGCTTGTCGGCGGCCCGCCAGTCGAGGCTTC
GGCCGCCGCGCTGGCCGGCGACGCCGCGGGCGCATGGCGGACCGCGGCCGTCGAGCTTGCGCGAGCGTTGGTCCGCGCTG
TGGCGGAGTCGCACGGCGTCGCGGCCGTTTTGTTCGCCGCGACGGCCGCCGCGGCGGCGGCCGTCGACCGGGGTGATCCG
CCGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A045IHC4

Antitoxin

Source ID Structure
AlphaFold DB A0A806JR81

References