Detailed information of TA system TA084760

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/COG4683-HTH_37
• Location: 3550265..3550935
• Replicon: chromosome
• Accession: NZ_CP023623
• Organism: Mycobacterium tuberculosis strain MDRMA203

Toxin (Protein)

• Gene name: higB
• Uniprot ID: O53332
• Locus tag: CP479_RS17400
• Protein ID: WP_003899954.1
• Coordinates: 3550265..3550609 (+)
• Length: 115 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: G0THF6
• Locus tag: CP479_RS17405
• Protein ID: WP_003899955.1
• Coordinates: 3550606..3550935 (+)
• Length: 110 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CP479_RS17365	3545338..3546198	+	861	WP_003416628.1	alpha/beta hydrolase	-
CP479_RS17370	3546173..3546688	+	516	WP_003899950.1	nitroreductase family deazaflavin-dependent oxidoreductase	-
CP479_RS17380	3546928..3547221	+	294	WP_003416635.1	hypothetical protein	-
CP479_RS17385	3547509..3548798	+	1290	WP_003416640.1	ATP-binding protein	-
CP479_RS17390	3549145..3549579	-	435	WP_003899952.1	type II toxin-antitoxin system VapC family toxin	-
CP479_RS17395	3549582..3550034	-	453	WP_003899953.1	type II toxin-antitoxin system Phd/YefM family antitoxin	-
CP479_RS17400	3550265..3550609	+	345	WP_003899954.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
CP479_RS17405	3550606..3550935	+	330	WP_003899955.1	XRE family transcriptional regulator	Antitoxin
CP479_RS17410	3551172..3552433	+	1262	WP_087902221.1	IS3-like element IS987 family transposase	-
CP479_RS17415	3552655..3553916	+	1262	WP_087902221.1	IS3-like element IS987 family transposase	-
CP479_RS17420	3554189..3554536	+	348	WP_003899956.1	DUF2384 domain-containing protein	-
CP479_RS17425	3554533..3555153	+	621	WP_003899957.1	RES family NAD+ phosphorylase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	-	3532834..3606925	74091

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-like (12-103)

Antitoxin

• Cro/C1-type helix-turn-helix domain (38-91)

Sequences

Toxin

Protein

• Length: 115 a.a.
• Molecular weight: 12692.50 Da
• Isoelectric Point: 5.6920

>T84760 WP_003899954.1 NZ_CP023623:3550265-3550609 [Mycobacterium tuberculosis]
MAVILLPQVERWFFALNRDAMASVTGAIDLLEMEGPTLGRPVVDKVNDSTFHNMKELRPAGTSIRILFAFDPARQAILLL
GGDKAGNWKRWYDNNIPIADQRSENWLASEHGGG

Nucleotide

• Length: 345 bp

>T84760 NZ_CP023623:3550265-3550609 [Mycobacterium tuberculosis]
TTGGCCGTGATCCTGCTCCCGCAGGTCGAACGGTGGTTCTTCGCGCTCAACAGGGATGCGATGGCCTCGGTCACCGGCGC
CATCGACCTGCTCGAAATGGAGGGGCCGACGTTGGGCCGCCCGGTGGTCGACAAAGTGAACGACTCAACGTTTCACAACA
TGAAGGAGCTGCGCCCCGCCGGCACCAGCATCCGGATCCTGTTCGCCTTCGACCCGGCCCGGCAGGCGATCCTGCTGCTG
GGCGGTGACAAGGCAGGCAACTGGAAACGCTGGTACGACAACAACATTCCAATCGCTGACCAGCGCTCCGAGAACTGGCT
GGCGAGCGAGCACGGAGGTGGATGA

Antitoxin

Protein

• Length: 110 a.a.
• Molecular weight: 11802.46 Da
• Isoelectric Point: 7.4051

>AT84760 WP_003899955.1 NZ_CP023623:3550606-3550935 [Mycobacterium tuberculosis]
MTMARNWRDIRADAVAQGRVDLQRAAVAREEMRDAVLAHRLAEIRKALGHARQADVAALMGVSQARVSKLESGDLSHTEL
GTLQAYVAALGGHLRIVAEFGENTVELTA

Nucleotide

• Length: 330 bp

>AT84760 NZ_CP023623:3550606-3550935 [Mycobacterium tuberculosis]
ATGACCATGGCCCGCAACTGGCGTGACATTCGCGCCGATGCCGTCGCGCAGGGCCGCGTGGATCTGCAGCGGGCCGCCGT
GGCACGCGAGGAGATGCGCGATGCCGTCCTGGCGCACCGCCTGGCCGAGATCCGCAAGGCGCTAGGCCACGCACGTCAGG
CCGACGTCGCGGCGCTGATGGGGGTCTCTCAGGCCCGTGTCTCCAAGCTGGAGAGCGGCGACCTGTCCCACACCGAACTC
GGCACCCTGCAGGCCTACGTTGCCGCCCTGGGCGGGCACCTGCGCATCGTCGCTGAGTTCGGCGAAAATACTGTCGAGCT
GACCGCCTGA

Structures

Toxin

• AlphaFold DB: A0A7U4FBK2

Antitoxin

• AlphaFold DB: G0THF6