Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) unclassified/-
Location 2976375..2977023 Replicon chromosome
Accession NZ_CP023622
Organism Mycobacterium tuberculosis strain MDRDM827

Toxin (Protein)


Gene name novel[antitoxin] Uniprot ID P9WJ12
Locus tag CP478_RS14550 Protein ID WP_003899414.1
Coordinates 2976375..2976698 (-) Length 108 a.a.

Antitoxin (Protein)


Gene name novel[toxin] Uniprot ID P9WJ10
Locus tag CP478_RS14555 Protein ID WP_003899415.1
Coordinates 2976778..2977023 (-) Length 82 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
CP478_RS14530 2971949..2973210 + 1262 WP_087902221.1 IS3-like element IS987 family transposase -
CP478_RS14535 2973584..2975023 - 1440 WP_003899411.1 phage major capsid protein -
CP478_RS14540 2975031..2975564 - 534 WP_003899412.1 HK97 family phage prohead protease -
CP478_RS14545 2975717..2976208 - 492 WP_003900541.1 phage terminase small subunit P27 family -
CP478_RS14550 2976375..2976698 - 324 WP_003899414.1 type II toxin-antitoxin system toxin Toxin
CP478_RS14555 2976778..2977023 - 246 WP_003899415.1 type II toxin-antitoxin system antitoxin Antitoxin
CP478_RS14560 2977020..2978447 - 1428 WP_003899416.1 DUF3631 domain-containing protein -
CP478_RS14565 2978449..2978841 - 393 WP_003899417.1 DUF2742 domain-containing protein -
CP478_RS14570 2978838..2979098 - 261 WP_003899418.1 helix-turn-helix domain-containing protein -
CP478_RS14575 2979115..2979477 - 363 WP_003900543.1 hypothetical protein -
CP478_RS14580 2979480..2980607 - 1128 WP_003899420.1 site-specific integrase -
CP478_RS14585 2980752..2980979 - 228 WP_003899421.1 hypothetical protein -
CP478_RS14590 2980976..2981365 - 390 WP_003899422.1 hypothetical protein -
CP478_RS14595 2981271..2981543 + 273 WP_003900544.1 hypothetical protein -
CP478_RS14600 2981642..2981875 + 234 WP_003413717.1 hypothetical protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
inside Prophage - - 2970340..2980607 10267


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Putative toxin of bacterial toxin-antitoxin pair

(1-85)

Antitoxin

GNAT domain

(17-121)


Sequences

Toxin        


Download         Length: 108 a.a.        Molecular weight: 12359.82 Da        Isoelectric Point: 8.6717

>T84691 WP_003899414.1 NZ_CP023622:c2976698-2976375 [Mycobacterium tuberculosis]
MTHKRTKRQPAIAAGLNAPRRNRVGRQHGWPADVPSAEQRRAQRQRDLEAIRRAYAEMVATSHEIDDDTAELALLSMHLD
DEQRRLEAGMKLGWHPYHFPDEPDSKQ

Download         Length: 324 bp

>T84691 NZ_CP023622:c2976698-2976375 [Mycobacterium tuberculosis]
TTGACCCACAAGCGCACTAAACGCCAGCCAGCCATCGCCGCAGGGCTCAACGCCCCGCGTCGGAATCGCGTTGGGCGGCA
ACATGGTTGGCCGGCCGACGTTCCGTCCGCCGAGCAGCGCCGCGCCCAACGGCAGCGCGACCTCGAGGCTATCCGCCGAG
CGTACGCCGAGATGGTGGCGACATCACACGAAATCGACGACGACACAGCCGAACTGGCGCTGTTGTCGATGCATCTCGAC
GATGAGCAGCGCCGGCTTGAGGCGGGGATGAAGCTCGGCTGGCATCCGTATCACTTCCCCGACGAACCCGACAGCAAACA
GTGA

Antitoxin


Download         Length: 82 a.a.        Molecular weight: 7697.71 Da        Isoelectric Point: 4.8565

>AT84691 WP_003899415.1 NZ_CP023622:c2977023-2976778 [Mycobacterium tuberculosis]
MSGHALAARTLLAAADELVGGPPVEASAAALAGDAAGAWRTAAVELARALVRAVAESHGVAAVLFAATAAAAAAVDRGDP
P

Download         Length: 246 bp

>AT84691 NZ_CP023622:c2977023-2976778 [Mycobacterium tuberculosis]
ATGAGCGGCCACGCGTTGGCTGCTCGGACGTTGCTGGCCGCCGCGGACGAGCTTGTCGGCGGCCCGCCAGTCGAGGCTTC
GGCCGCCGCGCTGGCCGGCGACGCCGCGGGCGCATGGCGGACCGCGGCCGTCGAGCTTGCGCGAGCGTTGGTCCGCGCTG
TGGCGGAGTCGCACGGCGTCGCGGCCGTTTTGTTCGCCGCGACGGCCGCCGCGGCGGCGGCCGTCGACCGGGGTGATCCG
CCGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A045IHC4

Antitoxin

Source ID Structure
AlphaFold DB A0A806JR81

References