Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) unclassified/-
Location 2976374..2977022 Replicon chromosome
Accession NZ_CP023620
Organism Mycobacterium tuberculosis strain LN1856

Toxin (Protein)


Gene name novel[antitoxin] Uniprot ID P9WJ12
Locus tag CP476_RS14545 Protein ID WP_003899414.1
Coordinates 2976374..2976697 (-) Length 108 a.a.

Antitoxin (Protein)


Gene name novel[toxin] Uniprot ID P9WJ10
Locus tag CP476_RS14550 Protein ID WP_003899415.1
Coordinates 2976777..2977022 (-) Length 82 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
CP476_RS14525 2971948..2973209 + 1262 WP_087902221.1 IS3-like element IS987 family transposase -
CP476_RS14530 2973583..2975022 - 1440 WP_003899411.1 phage major capsid protein -
CP476_RS14535 2975030..2975563 - 534 WP_003899412.1 HK97 family phage prohead protease -
CP476_RS14540 2975716..2976207 - 492 WP_003900541.1 phage terminase small subunit P27 family -
CP476_RS14545 2976374..2976697 - 324 WP_003899414.1 type II toxin-antitoxin system toxin Toxin
CP476_RS14550 2976777..2977022 - 246 WP_003899415.1 type II toxin-antitoxin system antitoxin Antitoxin
CP476_RS14555 2977019..2978446 - 1428 WP_003899416.1 DUF3631 domain-containing protein -
CP476_RS14560 2978448..2978840 - 393 WP_003899417.1 DUF2742 domain-containing protein -
CP476_RS14565 2978837..2979097 - 261 WP_003899418.1 helix-turn-helix domain-containing protein -
CP476_RS14570 2979114..2979476 - 363 WP_003900543.1 hypothetical protein -
CP476_RS14575 2979479..2980606 - 1128 WP_003899420.1 site-specific integrase -
CP476_RS14580 2980751..2980978 - 228 WP_003899421.1 hypothetical protein -
CP476_RS14585 2980975..2981364 - 390 WP_003899422.1 hypothetical protein -
CP476_RS14590 2981270..2981542 + 273 WP_003900544.1 hypothetical protein -
CP476_RS14595 2981641..2981874 + 234 WP_003413717.1 hypothetical protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
inside Prophage - - 2970339..2980606 10267


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, RelE/ParE toxin family

(4-91)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 108 a.a.        Molecular weight: 12359.82 Da        Isoelectric Point: 8.6717

>T84569 WP_003899414.1 NZ_CP023620:c2976697-2976374 [Mycobacterium tuberculosis]
MTHKRTKRQPAIAAGLNAPRRNRVGRQHGWPADVPSAEQRRAQRQRDLEAIRRAYAEMVATSHEIDDDTAELALLSMHLD
DEQRRLEAGMKLGWHPYHFPDEPDSKQ

Download         Length: 324 bp

>T84569 NZ_CP023620:c2976697-2976374 [Mycobacterium tuberculosis]
TTGACCCACAAGCGCACTAAACGCCAGCCAGCCATCGCCGCAGGGCTCAACGCCCCGCGTCGGAATCGCGTTGGGCGGCA
ACATGGTTGGCCGGCCGACGTTCCGTCCGCCGAGCAGCGCCGCGCCCAACGGCAGCGCGACCTCGAGGCTATCCGCCGAG
CGTACGCCGAGATGGTGGCGACATCACACGAAATCGACGACGACACAGCCGAACTGGCGCTGTTGTCGATGCATCTCGAC
GATGAGCAGCGCCGGCTTGAGGCGGGGATGAAGCTCGGCTGGCATCCGTATCACTTCCCCGACGAACCCGACAGCAAACA
GTGA

Antitoxin


Download         Length: 82 a.a.        Molecular weight: 7697.71 Da        Isoelectric Point: 4.8565

>AT84569 WP_003899415.1 NZ_CP023620:c2977022-2976777 [Mycobacterium tuberculosis]
MSGHALAARTLLAAADELVGGPPVEASAAALAGDAAGAWRTAAVELARALVRAVAESHGVAAVLFAATAAAAAAVDRGDP
P

Download         Length: 246 bp

>AT84569 NZ_CP023620:c2977022-2976777 [Mycobacterium tuberculosis]
ATGAGCGGCCACGCGTTGGCTGCTCGGACGTTGCTGGCCGCCGCGGACGAGCTTGTCGGCGGCCCGCCAGTCGAGGCTTC
GGCCGCCGCGCTGGCCGGCGACGCCGCGGGCGCATGGCGGACCGCGGCCGTCGAGCTTGCGCGAGCGTTGGTCCGCGCTG
TGGCGGAGTCGCACGGCGTCGCGGCCGTTTTGTTCGCCGCGACGGCCGCCGCGGCGGCGGCCGTCGACCGGGGTGATCCG
CCGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A045IHC4

Antitoxin

Source ID Structure
AlphaFold DB A0A806JR81

References