Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) unclassified/-
Location 2976523..2977171 Replicon chromosome
Accession NZ_CP023619
Organism Mycobacterium tuberculosis strain LN1100

Toxin (Protein)


Gene name novel[antitoxin] Uniprot ID P9WJ12
Locus tag CP475_RS14535 Protein ID WP_003899414.1
Coordinates 2976523..2976846 (-) Length 108 a.a.

Antitoxin (Protein)


Gene name novel[toxin] Uniprot ID P9WJ10
Locus tag CP475_RS14540 Protein ID WP_003899415.1
Coordinates 2976926..2977171 (-) Length 82 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
CP475_RS14515 2972097..2973358 + 1262 WP_087902221.1 IS3-like element IS987 family transposase -
CP475_RS14520 2973732..2975171 - 1440 WP_003899411.1 phage major capsid protein -
CP475_RS14525 2975179..2975712 - 534 WP_003899412.1 HK97 family phage prohead protease -
CP475_RS14530 2975865..2976356 - 492 WP_003900541.1 phage terminase small subunit P27 family -
CP475_RS14535 2976523..2976846 - 324 WP_003899414.1 type II toxin-antitoxin system toxin Toxin
CP475_RS14540 2976926..2977171 - 246 WP_003899415.1 type II toxin-antitoxin system antitoxin Antitoxin
CP475_RS14545 2977168..2978595 - 1428 WP_003899416.1 DUF3631 domain-containing protein -
CP475_RS14550 2978597..2978989 - 393 WP_003899417.1 DUF2742 domain-containing protein -
CP475_RS14555 2978986..2979246 - 261 WP_003899418.1 helix-turn-helix domain-containing protein -
CP475_RS14560 2979263..2979625 - 363 WP_003900543.1 hypothetical protein -
CP475_RS14565 2979628..2980755 - 1128 WP_003899420.1 site-specific integrase -
CP475_RS14570 2980900..2981127 - 228 WP_003899421.1 hypothetical protein -
CP475_RS14575 2981124..2981513 - 390 WP_003899422.1 hypothetical protein -
CP475_RS14580 2981419..2981691 + 273 WP_003900544.1 hypothetical protein -
CP475_RS14585 2981790..2982023 + 234 WP_003413717.1 hypothetical protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
inside Prophage - - 2970488..2980755 10267


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

HipA-like, C-terminal

(149-401)

Antitoxin

Cro/C1-type helix-turn-helix domain

(18-69)


Sequences

Toxin        


Download         Length: 108 a.a.        Molecular weight: 12359.82 Da        Isoelectric Point: 8.6717

>T84508 WP_003899414.1 NZ_CP023619:c2976846-2976523 [Mycobacterium tuberculosis]
MTHKRTKRQPAIAAGLNAPRRNRVGRQHGWPADVPSAEQRRAQRQRDLEAIRRAYAEMVATSHEIDDDTAELALLSMHLD
DEQRRLEAGMKLGWHPYHFPDEPDSKQ

Download         Length: 324 bp

>T84508 NZ_CP023619:c2976846-2976523 [Mycobacterium tuberculosis]
TTGACCCACAAGCGCACTAAACGCCAGCCAGCCATCGCCGCAGGGCTCAACGCCCCGCGTCGGAATCGCGTTGGGCGGCA
ACATGGTTGGCCGGCCGACGTTCCGTCCGCCGAGCAGCGCCGCGCCCAACGGCAGCGCGACCTCGAGGCTATCCGCCGAG
CGTACGCCGAGATGGTGGCGACATCACACGAAATCGACGACGACACAGCCGAACTGGCGCTGTTGTCGATGCATCTCGAC
GATGAGCAGCGCCGGCTTGAGGCGGGGATGAAGCTCGGCTGGCATCCGTATCACTTCCCCGACGAACCCGACAGCAAACA
GTGA

Antitoxin


Download         Length: 82 a.a.        Molecular weight: 7697.71 Da        Isoelectric Point: 4.8565

>AT84508 WP_003899415.1 NZ_CP023619:c2977171-2976926 [Mycobacterium tuberculosis]
MSGHALAARTLLAAADELVGGPPVEASAAALAGDAAGAWRTAAVELARALVRAVAESHGVAAVLFAATAAAAAAVDRGDP
P

Download         Length: 246 bp

>AT84508 NZ_CP023619:c2977171-2976926 [Mycobacterium tuberculosis]
ATGAGCGGCCACGCGTTGGCTGCTCGGACGTTGCTGGCCGCCGCGGACGAGCTTGTCGGCGGCCCGCCAGTCGAGGCTTC
GGCCGCCGCGCTGGCCGGCGACGCCGCGGGCGCATGGCGGACCGCGGCCGTCGAGCTTGCGCGAGCGTTGGTCCGCGCTG
TGGCGGAGTCGCACGGCGTCGCGGCCGTTTTGTTCGCCGCGACGGCCGCCGCGGCGGCGGCCGTCGACCGGGGTGATCCG
CCGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A045IHC4

Antitoxin

Source ID Structure
AlphaFold DB A0A806JR81

References