Detailed information of TA system    

insolicoBioinformatically predicted

Overview


TA module


Type II Classification (family/domain) higBA (relBE)/Gp49-HTH_37
Location 2201696..2202564 Replicon chromosome
Accession NZ_CP023616
Organism Mycobacterium tuberculosis strain LN3668

Toxin (Protein)


Gene name higB Uniprot ID P9WJA4
Locus tag CP472_RS10615 Protein ID WP_010886136.1
Coordinates 2201696..2202073 (+) Length 126 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID G0TLM0
Locus tag CP472_RS10620 Protein ID WP_003409886.1
Coordinates 2202115..2202564 (+) Length 150 a.a.

Genomic Context


Locus tag Coordinates Strand Size (bp) Protein ID Product Description
CP472_RS10565 2197485..2197937 - 453 WP_003899095.1 lipoprotein -
CP472_RS10570 2198001..2198402 + 402 WP_003409869.1 hypothetical protein -
CP472_RS10575 2198395..2198577 - 183 WP_003409870.1 hypothetical protein -
CP472_RS10580 2198691..2199041 - 351 WP_003899096.1 hypothetical protein -
CP472_RS10585 2199052..2199954 - 903 WP_003899097.1 hypothetical protein -
CP472_RS10590 2199975..2200166 - 192 WP_003409876.1 hypothetical protein -
CP472_RS10595 2200167..2200463 - 297 WP_003409877.1 hypothetical protein -
CP472_RS10600 2200703..2200918 + 216 WP_003409878.1 antitoxin -
CP472_RS10605 2200915..2201226 + 312 WP_003409881.1 type II toxin-antitoxin system VapC family toxin -
CP472_RS21805 2201200..2201721 - 522 WP_003904745.1 hypothetical protein -
CP472_RS10615 2201696..2202073 + 378 WP_010886136.1 type II toxin-antitoxin system toxin HigB Toxin
CP472_RS10620 2202115..2202564 + 450 WP_003409886.1 type II toxin-antitoxin system antitoxin HigA Antitoxin
CP472_RS10625 2202561..2203106 + 546 WP_003409891.1 SecB-like chaperone -
CP472_RS10630 2202995..2203609 - 615 WP_003901296.1 hypothetical protein -
CP472_RS10635 2203658..2203954 - 297 WP_003409896.1 type II toxin-antitoxin system RelE/ParE family toxin -
CP472_RS10640 2203951..2204202 - 252 WP_003409899.1 type II toxin-antitoxin system ParD family antitoxin -
CP472_RS10645 2204189..2204683 + 495 WP_003899099.1 hypothetical protein -
CP472_RS10650 2204843..2205250 - 408 WP_003409913.1 type II toxin-antitoxin system VapC family toxin -
CP472_RS10655 2205254..2205526 - 273 WP_003899100.1 type II toxin-antitoxin system Phd/YefM family antitoxin -
CP472_RS10660 2205559..2206779 - 1221 WP_003409919.1 TetR family transcriptional regulator Mce3R -

Associated MGEs


MGE
detail
Similar
MGEs
Relative
position
MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains


Predicted by InterproScan

Toxin

(27-113)

Antitoxin

(44-91)


Sequences


Toxin        


Download         Length: 126 a.a.        Molecular weight: 14397.77 Da        Isoelectric Point: 10.8862

>T84309 WP_010886136.1 NZ_CP023616:2201696-2202073 [Mycobacterium tuberculosis]
VPPPDPAAMGTWKFFRASVDGRPVFKKEFDKLPDQARAALIVLMQRYLVGDLAAGSIKPIRGDILELRWHEANNHFRVLF
FRWGQHPVALTAFYKNQQKTPKTKIETALDRQKIWKRAFGDTPPI

Download         Length: 378 bp

>T84309 NZ_CP023616:2201696-2202073 [Mycobacterium tuberculosis]
GTGCCGCCCCCTGATCCAGCCGCCATGGGTACCTGGAAGTTCTTCCGGGCATCTGTGGATGGCCGGCCGGTATTCAAGAA
GGAGTTCGACAAGCTTCCTGATCAGGCCCGGGCCGCGCTGATCGTGCTAATGCAGCGGTATCTCGTCGGCGACCTCGCCG
CAGGGAGCATCAAACCGATTCGTGGCGACATTCTGGAGTTGCGATGGCATGAGGCGAACAACCACTTCCGGGTACTGTTC
TTCCGCTGGGGCCAGCATCCCGTAGCGCTGACAGCGTTCTACAAGAACCAGCAGAAGACTCCCAAGACGAAGATCGAGAC
GGCCCTGGACCGGCAGAAAATCTGGAAAAGAGCCTTCGGCGACACCCCACCGATCTGA

Antitoxin


Download         Length: 150 a.a.        Molecular weight: 16760.16 Da        Isoelectric Point: 8.0771

>AT84309 WP_003409886.1 NZ_CP023616:2202115-2202564 [Mycobacterium tuberculosis]
MSIDFPLGDDLAGYIAEAIAADPSFKGTLEDAEEARRLVDALIALRKHCQLSQVEVAKRMGVRQPTVSGFEKEPSDPKLS
TLQRYARALDARLRLVLEVPTLREVPTWHRLSSYRGSARDHQVRVGADKEILMQTNWARHISVRQVEVA

Download         Length: 450 bp

>AT84309 NZ_CP023616:2202115-2202564 [Mycobacterium tuberculosis]
ATGAGCATTGACTTCCCTTTGGGTGACGACCTCGCCGGCTATATTGCCGAGGCGATTGCGGCTGATCCCAGCTTCAAAGG
CACTCTCGAAGACGCCGAGGAGGCACGCAGGCTGGTCGATGCGCTGATTGCGCTGCGCAAGCACTGCCAGCTGAGCCAGG
TTGAGGTTGCTAAGCGTATGGGGGTGCGCCAGCCCACCGTGAGCGGTTTCGAGAAGGAACCCAGCGACCCCAAACTGTCT
ACGCTGCAACGTTATGCCCGTGCATTGGACGCCCGGCTGCGGCTGGTGCTCGAAGTTCCCACGCTTCGCGAAGTGCCTAC
GTGGCATCGGCTCTCCTCTTATCGGGGCTCCGCACGGGACCACCAGGTCCGGGTGGGTGCAGACAAGGAAATCCTGATGC
AGACGAACTGGGCCCGCCACATTTCGGTTCGGCAGGTTGAGGTGGCATGA

Similar Proteins


Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures


Toxin

Source ID Structure
PDB 7AWK
PDB 7NBU


Antitoxin

Source ID Structure
PDB 5MTW
AlphaFold DB G0TLM0

References