Detailed information of TA system TA083949

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/COG4683-HTH_37
• Location: 2266682..2267601
• Replicon: chromosome
• Accession: NZ_CP023610
• Organism: Mycobacterium tuberculosis strain LN317

Toxin (Protein)

• Gene name: HigB2
• Uniprot ID: L7N4R2
• Locus tag: CP466_RS11030
• Protein ID: WP_003900449.1
• Coordinates: 2266996..2267601 (-)
• Length: 202 a.a.

Antitoxin (Protein)

• Gene name: HigA2
• Uniprot ID: L7N5K9
• Locus tag: CP466_RS11025
• Protein ID: WP_003410124.1
• Coordinates: 2266682..2266987 (-)
• Length: 102 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CP466_RS10990	2261693..2262949	-	1257	WP_003410095.1	HNH endonuclease	-
CP466_RS11000	2263303..2263878	+	576	WP_096872171.1	hypothetical protein	-
CP466_RS11005	2263875..2264915	+	1041	WP_003410103.1	ImmA/IrrE family metallo-endopeptidase	-
CP466_RS11010	2265157..2265876	+	720	WP_003410108.1	DUF433 domain-containing protein	-
CP466_RS11015	2265866..2266282	+	417	WP_003410114.1	hypothetical protein	-
CP466_RS11020	2266298..2266597	-	300	WP_096863739.1	hypothetical protein	-
CP466_RS11025	2266682..2266987	-	306	WP_003410124.1	XRE family transcriptional regulator	Antitoxin
CP466_RS11030	2266996..2267601	-	606	WP_003900449.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
CP466_RS11035	2267626..2267985	-	360	WP_003410131.1	hypothetical protein	-
CP466_RS11040	2268145..2268603	-	459	Protein_2087	hypothetical protein	-
CP466_RS11045	2268600..2270117	-	1518	Protein_2088	DEAD/DEAH box helicase	-
CP466_RS11050	2270627..2271625	-	999	WP_003410146.1	cation diffusion facilitator family transporter	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-like (86-181)

Antitoxin

• HigA2-like, helix-turn-helix domain (32-92)

Sequences

Toxin

Protein

• Length: 202 a.a.
• Molecular weight: 22744.96 Da
• Isoelectric Point: 7.3073

>T83949 WP_003900449.1 NZ_CP023610:c2267601-2266996 [Mycobacterium tuberculosis]
VNVPWENAHGGALYCLIRGDEFSAWHRLLFQRPGCAESVLACRHFLDGSPVARCSYPEEYHPCVISRIALLCDSVGWTAD
VERISAWLNGLDRETYELVFAAIEVLEEEGPALGCPLVDTVRGSRHKNMKELRPGSQGRSEVRILFAFDPARQAIMLAAG
NKAGRWTQWYDEKIKAADEMFAEHLAQFEDTKPKRRKRKKG

Nucleotide

• Length: 606 bp

>T83949 NZ_CP023610:c2267601-2266996 [Mycobacterium tuberculosis]
GTGAACGTTCCATGGGAGAACGCGCACGGCGGCGCACTGTACTGCCTCATACGTGGGGATGAATTCTCGGCCTGGCACAG
GCTACTGTTTCAACGGCCCGGGTGCGCAGAGTCCGTCCTCGCCTGCCGGCATTTCCTCGACGGTAGCCCCGTCGCCCGCT
GCTCTTACCCGGAGGAATATCACCCTTGTGTGATATCGCGTATCGCGCTACTCTGCGACTCTGTGGGGTGGACGGCGGAT
GTGGAGCGCATCTCCGCCTGGCTCAACGGCTTGGACCGAGAGACCTACGAATTGGTGTTCGCAGCTATCGAGGTTCTCGA
GGAAGAGGGCCCCGCACTTGGCTGTCCTCTGGTGGACACGGTGCGTGGGTCGCGCCACAAGAACATGAAGGAGCTGCGTC
CAGGATCACAAGGGCGGTCCGAGGTCCGCATTCTGTTCGCCTTCGACCCGGCGCGGCAGGCGATCATGCTGGCCGCCGGT
AACAAGGCCGGGCGGTGGACCCAGTGGTACGACGAGAAGATCAAAGCGGCCGATGAGATGTTTGCCGAACATCTCGCACA
GTTCGAGGACACGAAGCCAAAGAGGCGCAAGCGCAAGAAAGGGTAA

Antitoxin

Protein

• Length: 102 a.a.
• Molecular weight: 11291.91 Da
• Isoelectric Point: 10.7482

>AT83949 WP_003410124.1 NZ_CP023610:c2266987-2266682 [Mycobacterium tuberculosis]
MAMTLRDMDAVRPVNREAVDRHKARMRDEVRAFRLRELRAAQSLTQVQVAALAHIRQSRVSSIENGDIGSAQVNTLRKYV
SALGGELDITVRLGDETFTLA

Nucleotide

• Length: 306 bp

>AT83949 NZ_CP023610:c2266987-2266682 [Mycobacterium tuberculosis]
ATGGCGATGACACTACGGGACATGGACGCCGTACGTCCCGTTAACCGGGAGGCGGTGGACCGGCACAAGGCACGAATGCG
CGATGAAGTGCGGGCATTCCGGCTCCGGGAGCTGCGCGCGGCTCAGTCACTCACCCAGGTCCAGGTGGCTGCGCTGGCGC
ACATCCGGCAGAGCCGGGTATCCAGCATCGAGAACGGCGACATCGGCTCCGCACAGGTCAACACACTGCGCAAATACGTG
TCCGCTCTTGGCGGAGAACTGGATATCACCGTCCGCCTGGGAGATGAGACATTCACCCTGGCATAG

Structures

Toxin

• AlphaFold DB: A0A7U4BV20

Antitoxin

• AlphaFold DB: A0A7U4BV30