Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/COG4683-HTH_37
Location 2266620..2267539 Replicon chromosome
Accession NZ_CP023609
Organism Mycobacterium tuberculosis strain LN55

Toxin (Protein)


Gene name HigB2 Uniprot ID -
Locus tag CP465_RS11025 Protein ID WP_024753682.1
Coordinates 2266934..2267539 (-) Length 202 a.a.

Antitoxin (Protein)


Gene name HigA2 Uniprot ID L7N5K9
Locus tag CP465_RS11020 Protein ID WP_003410124.1
Coordinates 2266620..2266925 (-) Length 102 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
CP465_RS10985 2261631..2262887 - 1257 WP_003902247.1 HNH endonuclease -
CP465_RS10995 2263241..2263816 + 576 WP_003410100.1 hypothetical protein -
CP465_RS11000 2263813..2264853 + 1041 WP_003410103.1 ImmA/IrrE family metallo-endopeptidase -
CP465_RS11005 2265095..2265814 + 720 WP_003410108.1 DUF433 domain-containing protein -
CP465_RS11010 2265804..2266220 + 417 WP_003410114.1 hypothetical protein -
CP465_RS11015 2266236..2266535 - 300 WP_096863739.1 hypothetical protein -
CP465_RS11020 2266620..2266925 - 306 WP_003410124.1 XRE family transcriptional regulator Antitoxin
CP465_RS11025 2266934..2267539 - 606 WP_024753682.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
CP465_RS11030 2267564..2267923 - 360 WP_003410131.1 hypothetical protein -
CP465_RS11035 2268083..2268541 - 459 Protein_2090 hypothetical protein -
CP465_RS11040 2268538..2270055 - 1518 Protein_2091 DEAD/DEAH box helicase -
CP465_RS11045 2270565..2271563 - 999 WP_003410146.1 cation diffusion facilitator family transporter -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin HigB-like

(86-181)

Antitoxin

HigA2-like, helix-turn-helix domain

(32-92)


Sequences

Toxin        


Download         Length: 202 a.a.        Molecular weight: 22743.97 Da        Isoelectric Point: 7.6272

>T83888 WP_024753682.1 NZ_CP023609:c2267539-2266934 [Mycobacterium tuberculosis]
VNVPWENAHGGALYCLIRGNEFSAWHRLLFQRPGCAESVLACRHFLDGSPVARCSYPEEYHPCVISRIALLCDSVGWTAD
VERISAWLNGLDRETYELVFAAIEVLEEEGPALGCPLVDTVRGSRHKNMKELRPGSQGRSEVRILFAFDPARQAIMLAAG
NKAGRWTQWYDEKIKAADEMFAEHLAQFEDTKPKRRKRKKG

Download         Length: 606 bp

>T83888 NZ_CP023609:c2267539-2266934 [Mycobacterium tuberculosis]
GTGAACGTTCCATGGGAGAACGCGCACGGCGGCGCACTGTACTGCCTCATACGTGGGAATGAATTCTCGGCCTGGCACAG
GCTACTGTTTCAACGGCCCGGGTGCGCAGAGTCCGTCCTCGCCTGCCGGCATTTCCTCGACGGTAGCCCCGTCGCCCGCT
GCTCTTACCCGGAGGAATATCACCCTTGTGTGATATCGCGTATCGCGCTACTCTGCGACTCTGTGGGGTGGACGGCGGAT
GTGGAGCGCATCTCCGCCTGGCTCAACGGCTTGGACCGAGAGACCTACGAATTGGTGTTCGCAGCTATCGAGGTTCTCGA
GGAAGAGGGCCCCGCACTTGGCTGTCCTCTGGTGGACACGGTGCGTGGGTCGCGCCACAAGAACATGAAGGAGCTGCGTC
CAGGATCACAAGGGCGGTCCGAGGTCCGCATTCTGTTCGCCTTCGACCCGGCGCGGCAGGCGATCATGCTGGCCGCCGGT
AACAAGGCCGGGCGGTGGACCCAGTGGTACGACGAGAAGATCAAAGCGGCCGATGAGATGTTTGCCGAACATCTCGCACA
GTTCGAGGACACGAAGCCAAAGAGGCGCAAGCGCAAGAAAGGGTAA

Antitoxin


Download         Length: 102 a.a.        Molecular weight: 11291.91 Da        Isoelectric Point: 10.7482

>AT83888 WP_003410124.1 NZ_CP023609:c2266925-2266620 [Mycobacterium tuberculosis]
MAMTLRDMDAVRPVNREAVDRHKARMRDEVRAFRLRELRAAQSLTQVQVAALAHIRQSRVSSIENGDIGSAQVNTLRKYV
SALGGELDITVRLGDETFTLA

Download         Length: 306 bp

>AT83888 NZ_CP023609:c2266925-2266620 [Mycobacterium tuberculosis]
ATGGCGATGACACTACGGGACATGGACGCCGTACGTCCCGTTAACCGGGAGGCGGTGGACCGGCACAAGGCACGAATGCG
CGATGAAGTGCGGGCGTTCCGGCTCCGGGAGCTGCGCGCGGCTCAGTCACTCACCCAGGTCCAGGTGGCTGCGCTGGCGC
ACATCCGGCAGAGCCGGGTATCCAGCATCGAGAACGGCGACATCGGCTCCGCACAGGTCAACACACTGCGCAAATACGTG
TCCGCTCTTGGCGGAGAACTGGATATCACCGTCCGCCTGGGAGATGAGACATTCACCCTGGCATAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure

Antitoxin

Source ID Structure
PDB 7EWC
PDB 7EWD
PDB 7EWE
AlphaFold DB A0A7U4BV30

References