Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) unclassified/-
Location 2976335..2976983 Replicon chromosome
Accession NZ_CP023607
Organism Mycobacterium tuberculosis strain LE371

Toxin (Protein)


Gene name novel[antitoxin] Uniprot ID P9WJ12
Locus tag CP463_RS14535 Protein ID WP_003899414.1
Coordinates 2976335..2976658 (-) Length 108 a.a.

Antitoxin (Protein)


Gene name novel[toxin] Uniprot ID P9WJ10
Locus tag CP463_RS14540 Protein ID WP_003899415.1
Coordinates 2976738..2976983 (-) Length 82 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
CP463_RS14515 2971909..2973170 + 1262 WP_087902221.1 IS3-like element IS987 family transposase -
CP463_RS14520 2973544..2974983 - 1440 WP_003899411.1 phage major capsid protein -
CP463_RS14525 2974991..2975524 - 534 WP_003899412.1 HK97 family phage prohead protease -
CP463_RS14530 2975677..2976168 - 492 WP_003900541.1 phage terminase small subunit P27 family -
CP463_RS14535 2976335..2976658 - 324 WP_003899414.1 type II toxin-antitoxin system toxin Toxin
CP463_RS14540 2976738..2976983 - 246 WP_003899415.1 type II toxin-antitoxin system antitoxin Antitoxin
CP463_RS14545 2976980..2978407 - 1428 WP_003899416.1 DUF3631 domain-containing protein -
CP463_RS14550 2978409..2978801 - 393 WP_096874009.1 DUF2742 domain-containing protein -
CP463_RS14555 2978798..2979058 - 261 WP_003899418.1 helix-turn-helix domain-containing protein -
CP463_RS14560 2979075..2979437 - 363 WP_003900543.1 hypothetical protein -
CP463_RS14565 2979440..2980567 - 1128 WP_003899420.1 site-specific integrase -
CP463_RS14570 2980712..2980939 - 228 WP_003899421.1 hypothetical protein -
CP463_RS14575 2980936..2981325 - 390 WP_003899422.1 hypothetical protein -
CP463_RS14580 2981231..2981503 + 273 WP_003900544.1 hypothetical protein -
CP463_RS14585 2981602..2981835 + 234 WP_003413717.1 hypothetical protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
inside Prophage - - 2970300..2980567 10267


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin


No domain identified.


Antitoxin

Cro/C1-type helix-turn-helix domain

(47-90)


Sequences

Toxin        


Download         Length: 108 a.a.        Molecular weight: 12359.82 Da        Isoelectric Point: 8.6717

>T83777 WP_003899414.1 NZ_CP023607:c2976658-2976335 [Mycobacterium tuberculosis]
MTHKRTKRQPAIAAGLNAPRRNRVGRQHGWPADVPSAEQRRAQRQRDLEAIRRAYAEMVATSHEIDDDTAELALLSMHLD
DEQRRLEAGMKLGWHPYHFPDEPDSKQ

Download         Length: 324 bp

>T83777 NZ_CP023607:c2976658-2976335 [Mycobacterium tuberculosis]
TTGACCCACAAGCGCACTAAACGCCAGCCAGCCATCGCCGCAGGGCTCAACGCCCCGCGTCGGAATCGCGTTGGGCGGCA
ACATGGTTGGCCGGCCGACGTTCCGTCCGCCGAGCAGCGCCGCGCCCAACGGCAGCGCGACCTCGAGGCTATCCGCCGAG
CGTACGCCGAGATGGTGGCGACATCACACGAAATCGACGACGACACAGCCGAACTGGCGCTGTTGTCGATGCATCTCGAC
GATGAGCAGCGCCGGCTTGAGGCGGGGATGAAGCTCGGCTGGCATCCGTATCACTTCCCCGACGAACCCGACAGCAAACA
GTGA

Antitoxin


Download         Length: 82 a.a.        Molecular weight: 7697.71 Da        Isoelectric Point: 4.8565

>AT83777 WP_003899415.1 NZ_CP023607:c2976983-2976738 [Mycobacterium tuberculosis]
MSGHALAARTLLAAADELVGGPPVEASAAALAGDAAGAWRTAAVELARALVRAVAESHGVAAVLFAATAAAAAAVDRGDP
P

Download         Length: 246 bp

>AT83777 NZ_CP023607:c2976983-2976738 [Mycobacterium tuberculosis]
ATGAGCGGCCACGCGTTGGCTGCTCGGACGTTGCTGGCCGCCGCGGACGAGCTTGTCGGCGGCCCGCCAGTCGAGGCTTC
GGCCGCCGCGCTGGCCGGCGACGCCGCGGGCGCATGGCGGACCGCGGCCGTCGAGCTTGCGCGAGCGTTGGTCCGCGCTG
TGGCGGAGTCGCACGGCGTCGCGGCCGTTTTGTTCGCCGCGACGGCCGCCGCGGCGGCGGCCGTCGACCGGGGTGATCCG
CCGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A045IHC4

Antitoxin

Source ID Structure
AlphaFold DB A0A806JR81

References